Target Name
PRKC, apoptosis, WT1, regulator
Alternative Names
2310001G03Rik ELISA Kit; PAR 4 ELISA Kit; PAR-4 ELISA Kit; Pawr ELISA Kit; PAWR_HUMAN ELISA Kit; PRKC Apoptosis WT1 Regulator ELISA Kit; PRKC apoptosis WT1 regulator protein ELISA Kit; Prostate apoptosis response 4 protein ELISA Kit; Prostate apoptosis response protein 4 ELISA Kit; prostate apoptosis response protein PAR-4 ELISA Kit; Transcriptional repressor Par-4-like protein PAWR ELISA Kit; Transcriptional repressor PAR4 ELISA Kit; WT1 Interacting Protein ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
25 pg/mL-1600 pg/mL
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PAWR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
1:1 | Average % | 85 | |
Range % | 80-91 | |
1:2 | Average % | 90 | |
Range % | 84-98 | |
1:4 | Average % | 92 | |
Range % | 86-100 | |
1:8 | Average % | 95 | |
Range % | 88-101 | |
Recovery
The recovery of human PAWR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
Sample Type | Average % Recovery | Range | |
Serum (n=5) | 97 | 90-102 | |
EDTA plasma (n=4) | 94 | 90-100 | |
| | | | | | |
| | | | | | |
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
pg/ml | OD1 | OD2 | Average | Corrected | | 1600 | 2.745 | 2.652 | 2.699 | 2.569 | | 800 | 2.246 | 2.176 | 2.211 | 2.081 | | 400 | 1.761 | 1.704 | 1.733 | 1.603 | | 200 | 1.167 | 1.139 | 1.153 | 1.023 | | 100 | 0.524 | 0.569 | 0.547 | 0.417 | | 50 | 0.312 | 0.309 | 0.311 | 0.181 | | 25 | 0.198 | 0.189 | 0.194 | 0.064 | | 0 | 0.127 | 0.133 | 0.130 | | |
|
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Human PAWR ELISA Kit was designed for the quantitative measurement of Human PAWR protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL .