Human Protein Wnt-5b(WNT5B) ELISA kit

Instructions
Code CSB-EL026139HU
Size 96T,5×96T,10×96T

Product Details

Target Name wingless-type MMTV integration site family, member 5B
Alternative Names MGC2648, WNT-5B protein
Abbreviation WNT5B
Uniprot No. Q9H1J7
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human WNT5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %87-96
1:2Average %98
Range %94-102
1:4Average %94
Range %89-98
1:8Average %85
Range %80-89
Recovery
The recovery of Human WNT5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-102
EDTA plasma (n=4)8580-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.663 2.767 2.715 2.616
52.326 2.225 2.276 2.177
2.51.675 1.578 1.627 1.528
1.251.085 1.022 1.054 0.955
0.6250.507 0.523 0.515 0.416
0.3120.264 0.276 0.270 0.171
0.1560.178 0.187 0.183 0.084
00.099 0.098 0.099
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Database Links

HGNC: 16265

OMIM: 606361

KEGG: hsa:81029

STRING: 9606.ENSP00000308887

UniGene: Hs.306051

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