Human Protein Wnt-5b(WNT5B) ELISA kit

Instructions
Code CSB-EL026139HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name wingless-type MMTV integration site family, member 5B
Alternative Names MGC2648 ELISA Kit; Protein Wnt-5b ELISA Kit; Wingless type MMTV integration site family, member 5B ELISA Kit; WNT 5B ELISA Kit; WNT 5B protein ELISA Kit; Wnt family member 5B ELISA Kit; Wnt5b ELISA Kit; WNT5B protein ELISA Kit; WNT5B_HUMAN ELISA Kit
Abbreviation WNT5B
Uniprot No. Q9H1J7
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human WNT5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %87-96
1:2Average %98
Range %94-102
1:4Average %94
Range %89-98
1:8Average %85
Range %80-89
Recovery
The recovery of Human WNT5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-102
EDTA plasma (n=4)8580-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.663 2.767 2.715 2.616
52.326 2.225 2.276 2.177
2.51.675 1.578 1.627 1.528
1.251.085 1.022 1.054 0.955
0.6250.507 0.523 0.515 0.416
0.3120.264 0.276 0.270 0.171
0.1560.178 0.187 0.183 0.084
00.099 0.098 0.099
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Gene References into Functions
  1. exaggerated WNT-5B expression upon cigarette smoke exposure in the bronchial epithelium of COPD patients leads to TGF-beta/Smad3-dependent expression of genes related to airway remodelling PMID: 27126693
  2. the data suggest that WNT5B induces tube formation by regulating the expression of Snail and Slug proteins through activation of canonical and non-canonical WNT signalling pathways. PMID: 27593938
  3. Noncanonical Wnt signaling via Wnt5a/5b/11 may have role in the pathogenesis of aortic valve calcification. PMID: 27932350
  4. WNT-5B induces IL-6 and CXCL8 secretion in pulmonary fibroblasts. PMID: 27036869
  5. overexpression of WNT5B significantly increased cell proliferation, migration and invasion capacities of the COLO 205 cells in vitro. WNT5B may play an important role in the tumorigenesis of colorectal cancer. PMID: 27121420
  6. results suggest that Wnt5b-associated exosomes promote cancer cell migration and proliferation in a paracrine manner PMID: 27762090
  7. these results suggest that TGF-beta1 stimulates HSC-4 cell invasion through the Slug/Wnt-5b/MMP-10 signalling axis. PMID: 26861993
  8. This study identified WNT5B and CTNNBL1 for peak bone mineral density and body composition in males from the Han Chinese ethnic group. PMID: 26733379
  9. The interaction between Wnt isoforms and their LRP6 cognate receptor depends on the jutting loop present in Wnt3a but absent in Wnt5b. PMID: 25425204
  10. Results demonstrate that the Wnt5B pathway may play an important role in atypical teratoid rhabdoid tumor biology. PMID: 25246426
  11. Report increased Wnt5b levels in adriamycin treated liver cancer stem cells within 4h of treatment. PMID: 24944491
  12. This study shows that the WNT5B germline variant rs2010851 was significantly identified as a stage-dependent prognostic marker for CC patients after 5-fluorouracil-based adjuvant therapy. PMID: 23817222
  13. WNT5B is elevated in both in the tumor and the patients' serum from triple negative breast cancer. PMID: 24564888
  14. Knocking down Wnt5b expression reduced phospho-PKCA levels and cell migration. PMID: 24841200
  15. Ursolic acid suppressed the expression of Wnt5alpha/beta and beta-catenin, inducing apoptosis in prostate cancer cells. PMID: 24399733
  16. These results indicate that Wnt5b is involved in the migration ability of OSCC cells through active Cdc42 and RhoA. PMID: 24220306
  17. WNT3 and WNT5B are critical factors, secreted from mesenchymal cancer cells, for instigating the epithelial cancer cell invasion. PMID: 24344732
  18. TGF-beta/Smad, integrin/integrin-linked kinase (ILK) and wnt/beta-catenin signaling pathways are involved in epithelial to mesenchymal transition in human renal fibrogenesis. PMID: 24040439
  19. Differentiation paralleled the activation of Wnt5/Calmodulin signalling by autocrine/paracrine intense secretion of Wnt5a and Wnt5b PMID: 21980498
  20. This is significantly related to fat cell differentiation, suggesting its possible involvement in the onset of diabetes mellitus type 2 and metabolic syndrome. PMID: 21766608
  21. WNT5b is upregulated by beta-estradiol in tumor cell lines PMID: 12165812
  22. WNT5B gene may contribute to conferring susceptibility to type 2 diabetes and may be involved in the pathogenesis of this disease through the regulation of adipocyte function. PMID: 15386214
  23. In leiomyoma cells, the regulation of Wnt5b expression by retinoids appears to be attenuated. PMID: 15972578
  24. Variation in WNT5B predisposes to type 2 diabetes in the absence of obesity. PMID: 18555673

Show More

Hide All

Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Database Links

HGNC: 16265

OMIM: 606361

KEGG: hsa:81029

STRING: 9606.ENSP00000308887

UniGene: Hs.306051

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1