Human Protein prune homolog(PRUNE) ELISA kit

Instructions
Code CSB-EL018831HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name prune homolog (Drosophila)
Alternative Names DRES-17 ELISA Kit; DRES17 ELISA Kit; Drosophila-related expressed sequence 17 ELISA Kit; hPrune ELISA Kit; HTcD37 ELISA Kit; Protein prune homolog ELISA Kit; PRUNE ELISA Kit; Prune homolog ELISA Kit; PRUNE like protein ELISA Kit; PRUNE_HUMAN ELISA Kit; TcD37 homolog ELISA Kit
Abbreviation PRUNE
Uniprot No. Q86TP1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PRUNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %83-94
1:2Average %96
Range %91-104
1:4Average %89
Range %93-96
1:8Average %94
Range %86-98
Recovery
The recovery of human PRUNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-96
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30002.283 2.183 2.233 2.111
15001.647 1.567 1.607 1.485
7500.982 0.952 0.967 0.845
3750.532 0.512 0.522 0.400
187.50.384 0.374 0.379 0.257
940.247 0.237 0.242 0.120
470.199 0.189 0.194 0.072
00.122 0.121 0.122  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis
Gene References into Functions
  1. PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. PMID: 28334956
  2. These results further delineate a new PRUNE1-related syndrome, and highlight the importance of periodic data re-annotation in individuals who remain without a diagnosis after undergoing genome-wide testing. PMID: 28211990
  3. h-Prune expression is necessary for cancer cell motility and EMT and is associated with liver and lung metastasis in colorectal cancer cells. h-Prune could be a new prognostic marker and molecular target for CRLM. PMID: 27037526
  4. Together these studies define PRUNE as a molecule fundamental for normal human cortical development and define cellular and clinical consequences associated with PRUNE mutation PMID: 28334956
  5. h-prune is frequently expressed in anaplastic thyroid cancer cells and lymph nodes metastasis, and promotes migration and invasion of anaplastic thyroid cancer cells and metastasis in an anaplastic thyroid cancer model. PMID: 27109060
  6. Authors assessed h-Prune levels in peripheral blood of lung cancer patients using ELISA assay, showing that h-Prune is an early diagnostic marker for lung cancer. PMID: 25026278
  7. Data indicate that the D388A and D422A mutant h-Prune proteins interact weakly with Nm23-H1. PMID: 23448979
  8. Increased amplification of PRUNE is associated with T4 breast carcinoma. PMID: 20735841
  9. prune has a role in breast neoplasm aggressiveness PMID: 15671547
  10. GSK-3 and h-prune cooperatively regulate the disassembly of focal adhesions to promote cell migration and that h-prune is useful as a marker for tumor aggressiveness. PMID: 16428445
  11. Prune is composed of two independent active sites and two interaction sites for the assembly of oligomeric signalling complexes PMID: 17655525

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Involvement in disease Neurodevelopmental disorder with microcephaly, hypotonia, and variable brain anomalies (NMIHBA)
Subcellular Location Cytoplasm, Nucleus, Cell junction, focal adhesion
Protein Families PPase class C family, Prune subfamily
Tissue Specificity Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.
Database Links

HGNC: 13420

OMIM: 617413

KEGG: hsa:58497

STRING: 9606.ENSP00000271620

UniGene: Hs.78524

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