PRUNE1 Antibody

Datasheet

Code	CSB-PA771430LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PRUNE1 antibody at 4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 43, 31, 27, 25, 19 kDa Observed band size: 60 kDa IHC image of CSB-PA771430LA01HU diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA771430LA01HU at 1:135, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating PRUNE1 in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA771430LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA771430LA01HU (8µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PRUNE1 Polyclonal antibody

Uniprot No.

Q86TP1

Target Names

PRUNE1

Alternative Names

DRES-17 antibody; DRES17 antibody; Drosophila-related expressed sequence 17 antibody; hPrune antibody; HTcD37 antibody; Protein prune homolog antibody; PRUNE antibody; Prune homolog antibody; PRUNE like protein antibody; PRUNE_HUMAN antibody; TcD37 homolog antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Exopolyphosphatase PRUNE1 protein (1-168AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PRUNE1 Antibody (Product code: CSB-PA771430LA01HU) is Non-conjugated. For PRUNE1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA771430LB01HU	PRUNE1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA771430LC01HU	PRUNE1 Antibody, FITC conjugated
Biotin	CSB-PA771430LD01HU	PRUNE1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization.

Gene References into Functions

PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. PMID: 28334956
These results further delineate a new PRUNE1-related syndrome, and highlight the importance of periodic data re-annotation in individuals who remain without a diagnosis after undergoing genome-wide testing. PMID: 28211990
h-Prune expression is necessary for cancer cell motility and EMT and is associated with liver and lung metastasis in colorectal cancer cells. h-Prune could be a new prognostic marker and molecular target for CRLM. PMID: 27037526
Together these studies define PRUNE as a molecule fundamental for normal human cortical development and define cellular and clinical consequences associated with PRUNE mutation PMID: 28334956
h-prune is frequently expressed in anaplastic thyroid cancer cells and lymph nodes metastasis, and promotes migration and invasion of anaplastic thyroid cancer cells and metastasis in an anaplastic thyroid cancer model. PMID: 27109060
Authors assessed h-Prune levels in peripheral blood of lung cancer patients using ELISA assay, showing that h-Prune is an early diagnostic marker for lung cancer. PMID: 25026278
Data indicate that the D388A and D422A mutant h-Prune proteins interact weakly with Nm23-H1. PMID: 23448979
Increased amplification of PRUNE is associated with T4 breast carcinoma. PMID: 20735841
prune has a role in breast neoplasm aggressiveness PMID: 15671547
GSK-3 and h-prune cooperatively regulate the disassembly of focal adhesions to promote cell migration and that h-prune is useful as a marker for tumor aggressiveness. PMID: 16428445
Prune is composed of two independent active sites and two interaction sites for the assembly of oligomeric signalling complexes PMID: 17655525

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Involvement in disease

Neurodevelopmental disorder with microcephaly, hypotonia, and variable brain anomalies (NMIHBA)

Subcellular Location

Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed.

Protein Families

PPase class C family, Prune subfamily

Tissue Specificity

Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.

Database Links

HGNC: 13420

OMIM: 617413

KEGG: hsa:58497

STRING: 9606.ENSP00000271620

UniGene: Hs.78524

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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