Human SLAM family member 7(SLAMF7) ELISA kit

Instructions
Code CSB-EL021369HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name SLAM family member 7
Alternative Names SLAMF7 ELISA Kit; CS1 ELISA Kit; UNQ576/PRO1138 ELISA Kit; SLAM family member 7 ELISA Kit; CD2 subset 1 ELISA Kit; CD2-like receptor-activating cytotoxic cells ELISA Kit; CRACC ELISA Kit; Membrane protein FOAP-12 ELISA Kit; Novel Ly9 ELISA Kit; Protein 19A ELISA Kit; CD antigen CD319 ELISA Kit
Abbreviation SLAMF7
Uniprot No. Q9NQ25
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SLAMF7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %106
Range %100-112
1:10Average %98
Range %84-102
1:20Average %84
Range %80-88
1:40Average %95
Range %90-100
Recovery
The recovery of human SLAMF7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-98
EDTA plasma (n=4)9994-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
50002.396 2.501 2.449 2.272
25001.907 1.824 1.866 1.689
12501.295 1.234 1.265 1.088
6250.783 0.743 0.763 0.586
3120.462 0.448 0.455 0.278
1560.365 0.342 0.354 0.177
780.259 0.247 0.253 0.076
00.174 0.180 0.177
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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 Q&A
Q:

Per customers request, please provide the following information regarding Human SLAM family member 7, SLAMF7 ELISA Kit CSB-EL021369HU
1)The active ingredient of the stop solution and its concentration ?
2)The type, concentration, and state (liquid/lyophilized) of the preservatives used ?
3) If the components are lyophilized, what is the concentration prior to reconstitution (i.e. mass of preservative used/ total mass of lyophilized vial contents)?
4)Expression host of the standards ?
5)Which components have H202 with the concentration of H202 in each, if any?
6) Are there any components that contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) in any of the components in this kit?
7) If yes, which components contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) and the concentration?
Will the information always be the same for all of your competitive kits ? what about your sandwich kits?

A:
Thanks for your inquiry!
CSB-EL021369HU
Stop solution is 4N sulfuric acid.
Standard is NS0-derived recombinant protein. It is lyophilized powder. The concentration after reconsititution is 5000pg/mL. The preservative is liquid proclin 300(1:3000).
This kit doesn't contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide).
This information is not the same for all our kits. It is better to send us inquiry for a specific kit.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway
Gene References into Functions
  1. Memory CD8+ T cells from SLE patients displayed decreased amounts of SLAMF7, a surface receptor that characterizes effector CD8+ T cells. Ligation of SLAMF7 increased CD8+ T cell degranulation capacity and the percentage of IFNgamma-producing cells in response to antigen challenge in SLE patients and healthy controls. SLAMF7 engagement promoted cytotoxic lysis of target cells in response to stimulation with viral antig... PMID: 28076903
  2. phagocytosis of haematopoietic tumour cells during SIRPalpha-CD47 blockade was strictly dependent on SLAM family receptors in vitro and in vivo; in both mouse and human cells, this function required a single SLAM family member, SLAMF7 (also known as CRACC, CS1, CD319), expressed on macrophages and tumour cell targets PMID: 28424516
  3. Cohort statistics revealed a significant increase of circulating sSLAMF7 in multiple myeloma patients versus normal controls PMID: 27116021
  4. Blimp-1 regulates the transcription of CS1 gene in NK and B cell lines from multiple myeloma and diffuse large B cell lymphoma patients. PMID: 26310579
  5. SLAMF7-triggered inhibition is mediated by a mechanism involving Src kinases, CD45, and SHIP-1 that is defective in MM cells PMID: 25312647
  6. Our data highlight the therapeutic potential of targeting CD319 in rheumatoid arthritis PMID: 24299175
  7. SLAMF7 plays an inhibitory role in human monocytes to control proinflammatory immune responses. PMID: 23695528
  8. These results suggest a role for CD319 and CD229 in the systemic lupus erythematosus disease process. PMID: 23956418
  9. These data suggest an involvement of CRACC-mediated NK cell activation in periodontal tissue destruction and point to a plausible distinction in the pathobiology of aggressive and chronic periodontitis. PMID: 23250953
  10. CS1-L and CS1-S may differentially regulate human NK cell functions PMID: 15368295
  11. CS1 may play a role in the regulation of B lymphocyte proliferation during immune responses PMID: 17878365
  12. CS1 was expressed at adhesion-promoting uropod membranes of polarized Multiple Myeloma cells, andis required for MM cell adhesion to bone marrow stromal cells PMID: 17906076
  13. HuLuc63 eliminates myeloma cells, at least in part, via NK-mediated ADCC and shows the therapeutic potential of targeting CS1 with HuLuc63 for the treatment of multiple myeloma. PMID: 18451245

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Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. Expressed
Database Links

HGNC: 21394

OMIM: 606625

KEGG: hsa:57823

STRING: 9606.ENSP00000357022

UniGene: Hs.517265

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