SLAMF7 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA021369MA1HU
Size	US$210
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Image	IHC image of CSB-RA021369MA1HU diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing A549 cells surface stained with CSB-RA021369MA1HU (red line) at 1:200. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/110⁶cells) used under the same conditions. Acquisition of >10,023 events was performed.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q9NQ25

Target Names

SLAMF7

Alternative Names

SLAMF7 antibody; CS1 antibody; UNQ576/PRO1138 antibody; SLAM family member 7 antibody; CD2 subset 1 antibody; CD2-like receptor-activating cytotoxic cells antibody; CRACC antibody; Membrane protein FOAP-12 antibody; Novel Ly9 antibody; Protein 19A antibody; CD antigen CD319 antibody

Species Reactivity

Human

Immunogen

Recombinant Human SLAMF7 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse IgG

Clone No.

7H1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SLAMF7, also known as CS1 or CD319, is a cell surface glycoprotein belonging to the SLAM family of immunomodulatory receptors. Expressed predominantly on natural killer cells, activated B cells, and plasma cells, SLAMF7 plays a critical role in immune cell activation and cytotoxicity. Its elevated expression on multiple myeloma cells has made it a compelling therapeutic target, with SLAMF7-directed antibodies now established in clinical oncology. For researchers investigating immune cell biology, tumor immunology, or therapeutic antibody mechanisms, reliable detection of SLAMF7 is essential.

This recombinant monoclonal antibody, clone 7H1, offers the consistency and reproducibility that demanding immunological studies require. Generated against recombinant human SLAMF7 protein and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation. This means your experimental conditions remain stable across long-term projects and multi-site collaborations, reducing the troubleshooting burden that can accompany traditional hybridoma-derived reagents.

Validation studies confirm robust performance across multiple platforms. Immunohistochemical staining of paraffin-embedded human lung cancer tissue demonstrates clear target detection using citrate buffer antigen retrieval, with effective results at dilutions from 1:20 to 1:200. Flow cytometry analysis using A549 cells shows distinct surface staining with clean separation from isotype control, confirming specificity for cell surface SLAMF7 detection in human samples.

Whether you are characterizing immune cell populations, investigating SLAMF7 expression patterns in tumor microenvironments, or validating therapeutic targeting strategies, this antibody delivers the flexibility to move seamlessly between tissue-based and cell-based workflows. The unconjugated format allows pairing with your preferred detection systems across immunology and oncology research applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells. May play a role in lymphocyte adhesion. In LPS-activated monocytes negatively regulates production of proinflammatory cytokines.; Isoform 3 does not mediate any NK cell activation.

Gene References into Functions

Memory CD8+ T cells from SLE patients displayed decreased amounts of SLAMF7, a surface receptor that characterizes effector CD8+ T cells. Ligation of SLAMF7 increased CD8+ T cell degranulation capacity and the percentage of IFNgamma-producing cells in response to antigen challenge in SLE patients and healthy controls. SLAMF7 engagement promoted cytotoxic lysis of target cells in response to stimulation with viral antig... PMID: 28076903
phagocytosis of haematopoietic tumour cells during SIRPalpha-CD47 blockade was strictly dependent on SLAM family receptors in vitro and in vivo; in both mouse and human cells, this function required a single SLAM family member, SLAMF7 (also known as CRACC, CS1, CD319), expressed on macrophages and tumour cell targets PMID: 28424516
Cohort statistics revealed a significant increase of circulating sSLAMF7 in multiple myeloma patients versus normal controls PMID: 27116021
Blimp-1 regulates the transcription of CS1 gene in NK and B cell lines from multiple myeloma and diffuse large B cell lymphoma patients. PMID: 26310579
SLAMF7-triggered inhibition is mediated by a mechanism involving Src kinases, CD45, and SHIP-1 that is defective in MM cells PMID: 25312647
Our data highlight the therapeutic potential of targeting CD319 in rheumatoid arthritis PMID: 24299175
SLAMF7 plays an inhibitory role in human monocytes to control proinflammatory immune responses. PMID: 23695528
These results suggest a role for CD319 and CD229 in the systemic lupus erythematosus disease process. PMID: 23956418
These data suggest an involvement of CRACC-mediated NK cell activation in periodontal tissue destruction and point to a plausible distinction in the pathobiology of aggressive and chronic periodontitis. PMID: 23250953
CS1-L and CS1-S may differentially regulate human NK cell functions PMID: 15368295
CS1 may play a role in the regulation of B lymphocyte proliferation during immune responses PMID: 17878365
CS1 was expressed at adhesion-promoting uropod membranes of polarized Multiple Myeloma cells, andis required for MM cell adhesion to bone marrow stromal cells PMID: 17906076
HuLuc63 eliminates myeloma cells, at least in part, via NK-mediated ADCC and shows the therapeutic potential of targeting CS1 with HuLuc63 for the treatment of multiple myeloma. PMID: 18451245

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. Expressed

Database Links

HGNC: 21394

OMIM: 606625

KEGG: hsa:57823

STRING: 9606.ENSP00000357022

UniGene: Hs.517265

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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