Human SPARC-related modular calcium-binding protein 1(SMOC1) ELISA kit

Instructions
Code CSB-EL021842HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name SPARC related modular calcium binding 1
Alternative Names Secreted modular calcium-binding protein 1 ELISA Kit; SMOC-1 ELISA Kit; SMOC1 ELISA Kit; SMOC1_HUMAN ELISA Kit; SPARC-related modular calcium-binding protein 1 ELISA Kit
Abbreviation SMOC1
Uniprot No. Q9H4F8
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 92-101  
1:2 Average % 88  
Range % 84-92  
1:4 Average % 90  
Range % 85-98  
1:8 Average % 106  
Range % 101-114  
Recovery
The recovery of pig KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-100  
EDTA plasma (n=4) 84 80-88  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 1.815 1.731 1.773 1.632  
100 1.128 1.173 1.151 1.010  
50 0.789 0.827 0.808 0.667  
25 0.503 0.526 0.515 0.374  
12.5 0.312 0.309 0.311 0.170  
6.25 0.251 0.271 0.261 0.120  
3.12 0.188 0.178 0.183 0.042  
0 0.144 0.138 0.141    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Citations

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Target Background

Function
(From Uniprot)
Plays essential roles in both eye and limb development. Probable regulator of osteoblast differentiation.
Gene References into Functions
  1. Missense mutation in exon 3 of SMOC1 segregated with the Waardenburg anophthalmia syndrome in the Iranian family. PMID: 28807869
  2. This is the first report of Waardenburg anophthalmia syndrome (WAS) caused by a SMOC1 variant in a Pakistani population. The mutation identified in the present investigation extends the body of evidence implicating the gene SMOC-1 in causing WAS. PMID: 28085523
  3. SMOC binds to Pro-EGF, but does not induce Erk phosphorylation via the EGFR. PMID: 27101391
  4. IL-17A but not IL-22 suppresses the replication of hepatitis B virus by inducing the expression of MxA and OAS. PMID: 23274784
  5. SMOC1 provides a link between prenatal hormone exposure and digit ratio. PMID: 23263445
  6. Homozygosity mapping and subsequent targeted mutation analysis of a locus on 14q24.2 identified homozygous mutations in SMOC1 (SPARC-related modular calcium binding 1) in eight unrelated families with Waardenburg Anophthalmia syndrome. PMID: 21750680
  7. The present study thus identified SPARC related modular calcium binding 1 as a new cancer-associated protein capable of interacting with tenascin-C in vitro PMID: 21349332
  8. these findings indicate that SMOC1/Smoc1 is essential for ocular and limb development in both humans and mice. PMID: 21194678
  9. Waardenburg anophthalmia syndrome is genetically heterogeneous; a second was locus found on chromosome 14, and mutations in SMOC1 were shown also cause this syndrome. PMID: 21194680
  10. Analyzed the secretory protein profiles of BMSCs grown in osteogenic medium (OSM) and identified SPARC-related modular calcium-binding protein 1 (SMOC1), a member of the SPARC family, as a regulator of osteoblast differentiation of BMSCs. PMID: 20359165
  11. isolation of the novel gene SMOC-1 encoding a secreted modular protein containing an EF-hand calcium-binding domain; localization within basement membranes in kidney and skeletal muscle and expression in the zona pellucida surrounding the oocyte PMID: 12130637
  12. SMOC-1 is of physiological interest because it codes a secreted glycoprotein with five domains, each containing regions homologous to those on other proteins that mediate cell-matrix interactions. PMID: 17386346

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Involvement in disease Ophthalmoacromelic syndrome (OAS)
Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Specificity Widely expressed in many tissues with a strongest signal in ovary. No expression in spleen.
Database Links

HGNC: 20318

OMIM: 206920

KEGG: hsa:64093

STRING: 9606.ENSP00000355110

UniGene: Hs.497349

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