Human SPARC-related modular calcium-binding protein 1(SMOC1) ELISA kit

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Target Background

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Product Details

Description

This Human SMOC1 ELISA Kit was designed for the quantitative measurement of Human SMOC1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

Target Name

SPARC related modular calcium binding 1

Alternative Names

Secreted modular calcium-binding protein 1 ELISA Kit; SMOC-1 ELISA Kit; SMOC1 ELISA Kit; SMOC1_HUMAN ELISA Kit; SPARC-related modular calcium-binding protein 1 ELISA Kit

Abbreviation

SMOC1

Uniprot No.

Q9H4F8

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	88
	Range %	84-92
1:4	Average %	90
	Range %	85-98
1:8	Average %	106
	Range %	101-114

Recovery

The recovery of pig KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	84	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	1.815	1.731	1.773	1.632
100	1.128	1.173	1.151	1.010
50	0.789	0.827	0.808	0.667
25	0.503	0.526	0.515	0.374
12.5	0.312	0.309	0.311	0.170
6.25	0.251	0.271	0.261	0.120
3.12	0.188	0.178	0.183	0.042
0	0.144	0.138	0.141

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Plays essential roles in both eye and limb development. Probable regulator of osteoblast differentiation.
Gene References into Functions	Missense mutation in exon 3 of SMOC1 segregated with the Waardenburg anophthalmia syndrome in the Iranian family. PMID: 28807869 This is the first report of Waardenburg anophthalmia syndrome (WAS) caused by a SMOC1 variant in a Pakistani population. The mutation identified in the present investigation extends the body of evidence implicating the gene SMOC-1 in causing WAS. PMID: 28085523 SMOC binds to Pro-EGF, but does not induce Erk phosphorylation via the EGFR. PMID: 27101391 IL-17A but not IL-22 suppresses the replication of hepatitis B virus by inducing the expression of MxA and OAS. PMID: 23274784 SMOC1 provides a link between prenatal hormone exposure and digit ratio. PMID: 23263445 Homozygosity mapping and subsequent targeted mutation analysis of a locus on 14q24.2 identified homozygous mutations in SMOC1 (SPARC-related modular calcium binding 1) in eight unrelated families with Waardenburg Anophthalmia syndrome. PMID: 21750680 The present study thus identified SPARC related modular calcium binding 1 as a new cancer-associated protein capable of interacting with tenascin-C in vitro PMID: 21349332 these findings indicate that SMOC1/Smoc1 is essential for ocular and limb development in both humans and mice. PMID: 21194678 Waardenburg anophthalmia syndrome is genetically heterogeneous; a second was locus found on chromosome 14, and mutations in SMOC1 were shown also cause this syndrome. PMID: 21194680 Analyzed the secretory protein profiles of BMSCs grown in osteogenic medium (OSM) and identified SPARC-related modular calcium-binding protein 1 (SMOC1), a member of the SPARC family, as a regulator of osteoblast differentiation of BMSCs. PMID: 20359165 isolation of the novel gene SMOC-1 encoding a secreted modular protein containing an EF-hand calcium-binding domain; localization within basement membranes in kidney and skeletal muscle and expression in the zona pellucida surrounding the oocyte PMID: 12130637 SMOC-1 is of physiological interest because it codes a secreted glycoprotein with five domains, each containing regions homologous to those on other proteins that mediate cell-matrix interactions. PMID: 17386346 Show More Hide All
Involvement in disease	Ophthalmoacromelic syndrome (OAS)
Subcellular Location	Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane.
Tissue Specificity	Widely expressed in many tissues with a strongest signal in ovary. No expression in spleen.
Database Links	HGNC: 20318 OMIM: 206920 KEGG: hsa:64093 STRING: 9606.ENSP00000355110 UniGene: Hs.497349

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Code	CSB-EL021842HU
Size	96T,5×96T,10×96T How to order?
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.