Human Sodium/potassium-transporting ATPase subunit alpha-1(ATP1A1) ELISA kit

Instructions
Code CSB-EL002322HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Alternative Names A1A1 ELISA Kit; AT1A1 ELISA Kit; AT1A1_HUMAN ELISA Kit; ATP1A1 ELISA Kit; Atpa-1 ELISA Kit; ATPase Na+/K+ transporting alpha 1 polypeptide ELISA Kit; ATPase Na+/K+ transporting subunit alpha 1 ELISA Kit; BC010319 ELISA Kit; EC 3.6.3.9 ELISA Kit; MGC3285 ELISA Kit; MGC38419 ELISA Kit; MGC51750 ELISA Kit; Na K ATPase alpha A catalytic polypeptide ELISA Kit; Na K ATPase catalytic subunit alpha A protein ELISA Kit; Na(+)/K(+) ATPase 1 ELISA Kit; Na(+)/K(+) ATPase alpha-1 subunit ELISA Kit; Na+; K+ ATPase alpha subunit ELISA Kit; Na+/K+ ATPase alpha 1 subunit ELISA Kit; Na+/K+ ATPase 1 ELISA Kit; Na,K ATPase alpha 1 subunit ELISA Kit; Nkaa1b ELISA Kit; Sodium potassium ATPase alpha 1 polypeptide ELISA Kit; Sodium pump 1 ELISA Kit; Sodium pump subunit alpha-1 ELISA Kit; sodium-potassium ATPase catalytic subunit alpha-1 ELISA Kit; Sodium/potassium-transporting ATPase subunit alpha-1 ELISA Kit
Abbreviation ATP1A1
Uniprot No. P05023
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ATP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %91
Range %80-100
1:2Average %96
Range %85-104
1:4Average %98
Range %92-104
1:8Average %96
Range %82-110
Recovery
The recovery of human ATP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9484-106
EDTA plasma (n=4)9890-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.685 2.697 2.691 2.534
5001.954 1.965 1.960 1.803
2501.104 1.125 1.115 0.958
1250.701 0.732 0.717 0.560
62.50.446 0.440 0.443 0.286
31.20.326 0.315 0.321 0.164
15.60.267 0.255 0.261 0.104
00.153 0.161 0.157  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Q&A and Customer Reviews

 Q&A
Q:

I'm interested in trying this product for use on Atlantic Salmon as the over protein sequence homology is over 80% so may well work. Do you have any details on the specific peptide sequence it was raised to?

A:
Thanks for your inquiry!
The 24T size of kit CSB-EL002322HU is available.
This kit CSB-EL002322HU is developed based on the human matrix, specifically human atp1a1.
Coated anti: Mouse monoclonal antibody
Detect anti: Goat polyclonal antibody
Immunogen: NS0-derived recombinant protein
We do not detect the epitope mapping on the antibodies, so we don't know the recognition area.
Even if you refers to the homology of more than 80%, which is based on the protein-level structure of the comparison, but most of the protein in the high-level structure (spatial structure) exists.
Therefore, if the antibody recognition region is just in the homologous domain, in theory, it can be detected, but this relates to the matrix differences, it is not recommended to do the test across the species. So sorry that currently the kit special for fish ATP1A1 is unavailable.

Target Data

Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Gene References into Functions
  1. mutations in ATP1A1, which encodes the alpha1 subunit of the Na(+),K(+)-ATPase, are identified as a cause of autosomal-dominant CMT2. PMID: 29499166
  2. Low ATP1A1 expression is associated with renal cell carcinoma. PMID: 30015972
  3. These data suggest the role of beta-amyloid as a novel physiological regulator of Na,K-ATPase. PMID: 27296892
  4. induction of a novel pathway (alpha1-AMPK-ULK1) induces autophagy as a host-directed strategy for HCMV inhibition.IMPORTANCE PMID: 29321306
  5. An antibody against the extracellular DR region (897-911) of Na(+)-K(+)-ATPase subunit alpha 1 disrupted the Na+-K+-ATPase/ROS amplifier and protected cultured cardiomyocytes from ROS-induced injury. PMID: 28181111
  6. Disruption of Ankyrin B and Caveolin-1 Interaction Sites Alters Na(+),K(+)-ATPase Membrane Diffusion PMID: 28988699
  7. Mutations in ATP1A1 cause the excessive autonomous aldosterone secretion of Aldosterone-producing Adenomas. PMID: 28584016
  8. Cardiotonic steroids activate NF-kappaB leading to proinflammatory cytokine production in primary macrophages through a signaling complex, including CD36, TLR4, and Na/K-ATPase. PMID: 28619997
  9. This study reports molecular dynamic simulations of the human NaK-ATPase alpha1 beta 1 isoform embedded into 1,2-oleoylphosphatidylcholine bilayer. PMID: 27966362
  10. Data show that the expression of aquaporin (AQP) 1, AQP3, AQP5, epithelial Na+ channel (ENaC) and sodium potassium ATPase (Na-K-ATPase) are altered in patients with acute respiratory failure (ARF) due to diffuse alveolar damage (DAD), and the cause of DAD does not seem to influence the level of impairment of these channels. PMID: 27835672
  11. Ouabain stimulates NKA in renal proximal tubule cells through an angiotensin/AT1R-dependent mechanism and that this pathway contributes to cardiac glycoside associated hypertension. PMID: 27496272
  12. Reduction in ATP1A1 expression levels is related to major depressive disorder anxiety score. PMID: 27487491
  13. Our current findings demonstrate that Arctigenin is an antagonist of MR and effectively decreases the Na/K-ATPase 1 gene expression. Our work provides a hint for the drug discovery against cardiovascular disease PMID: 26446917
  14. Data suggest that targeting Na(+)/K(+) ATPase alpha-1 subunit (ATP1A1) is a approach to the treatment of hepatocellular carcinoma (HCC). PMID: 26334094
  15. Mutations in ATP1A1 gene is associated with aldosterone-producing adenomas. PMID: 26285814
  16. Gal-3 interacts with ATP1A1 and induces the phosphorylation of MDR1, mediating multidrug resistance. PMID: 26158764
  17. protein kinase A and C activation can increase Na,K-ATPase activity in human muscle but not via P2Y receptor stimulation. PMID: 24614174
  18. Mutations of the ATP1A1 depolarize adrenocortical cells, disturb the K+ sensitivity, and lower intracellular pH but do not induce an overt increase of intracellular Ca2+. PMID: 26418325
  19. Different mutations (KCNJ5, ATP1A1, ATP2B3, and CACNA1D) are found in different aldosterone-producing nodules from the same adrenal, suggesting that somatic mutations are independent events triggered by mechanisms that remain to be identified. PMID: 26351028
  20. Data shows that a functional 12T-insertion polymorphism in the ATP1A1 promoter confers decreased susceptibility to hypertension in a male Sardinian population supporting ATP1A1 as a hypertension susceptibility gene in this population. PMID: 25615575
  21. Data show that the intracellular M4M5 loop of human alpha1 subunit of Na+,K+-ATPase (ATP1a1) interacts directly with rat rab GTP-binding protein Rab27a. PMID: 25051489
  22. Ebola VP24 protein plays a critical role in the evasion of the host immune response by interacting with human ATP1a1 protein. PMID: 25158218
  23. Studies indicate specific lipid-binding sites in Na,K-ATPase E1 and E2 crystal structures. PMID: 25791351
  24. Report tumor cell sensitivity to cardiac glycosides depends on pattern of expression of alpha1-, alpha2-, or alpha3-isoforms of Na-K-ATPase. PMID: 25994790
  25. ATP1A1-mediated Src signaling inhibits coronavirus entry into host cells. PMID: 25653449
  26. The cytoplasmic domain of ATP1A1 directly interacts with FGF2 and is required for FGF2 secretion. PMID: 25533462
  27. While JNK exerts an inhibitory effect on the ATPase, NF-kappaB increases its activity and abrogates the stimulatory effect of the sphingolipid on JNK leading thus to an additional increase in the ATPase activity. PMID: 24819540
  28. Somatic mutations found in KCNJ5, ATP1A1, and ATP2B3 appear to be the driving forces for a higher aldosterone production and proliferations of glomerulosa cells. PMID: 24179102
  29. H-K-ATPase alpha-subunit mRNA 3' UTR to binding of miR-1289 identifies a novel regulatory mechanism of gastric acid secretion and offers new insights into mechanisms underlying transient H. pylori-induced hypochlorhydria. PMID: 24503769
  30. JAK2 is a novel energy-sensing kinase that curtails energy consumption by downregulating Na(+)/K(+)-ATPase expression and activity. PMID: 24304834
  31. Data indicate that overexpression of ouabain-insensitive rat Na(+)/K(+)-ATPase alpha1 failed to inhibit internalization of human Na(+)/K(+)-ATPase alpha1 expressed in the same cells. PMID: 24275648
  32. ATP1A1 mutations are present in aldosterone-producing adenomas that result in an increase in CYP11B2 gene expression and may account for the dysregulated aldosterone production in a subset of patients with sporadic primary aldosteronism. PMID: 24082052
  33. Somatic mutations in either ATP1A1 and CACNA1D were found in a subset of adrenal aldosterone-producing adenomas with a zona glomerulosa-like phenotype. PMID: 23913004
  34. Evidence for a new mechanism by which hypercapnia via soluble adenylyl cyclase, cAMP, PKA Type Ialpha, and alpha-adducin regulates Na,K-ATPase endocytosis in alveolar epithelial cells. PMID: 23349050
  35. Somatic mutations in ATP1A1 gene leads to aldosterone-producing adenomas and secondary hypertension. PMID: 23416519
  36. ZNRF1 and ZNRF2 are new players in regulation of the ubiquitous Na(+)/K(+)ATPase that is tuned to changing demands in many physiological contexts. PMID: 22797923
  37. NRF-1 regulates Atp1a1 and Atp1b1 and are important in mediating the energy generation and neuronal activity. PMID: 23048038
  38. Ouabain could up-regulate Na+, K(+)-ATPase alpha1 subunit expression and reduce beta1-subunit expression which mediated signal transduction and decreased cell-cell adhesions and induced ECV304 cell death. PMID: 21141520
  39. Data show that Na(+),K(+)-ATPase activity was >50% lower and membrane-associated tubulin content was >200% higher in erythrocyte membranes from diabetic patients. PMID: 22565168
  40. A significant increase in the expression of ATP1A1, which encodes the alpha1-subunit of the Na(+)/K(+)-ATPase, was identified in HNF1B patients consistent with its role in Mg(2+) homeostasis. PMID: 22269832
  41. the sodium pump alpha1 sub-unit has a role in progression of metastatic melanoma PMID: 19243476
  42. FXYD1 raises the affinity of the human alpha1beta1 isoform of Na,K-ATPase for Na ions PMID: 21449573
  43. There was overexpression of the alpha1 or alpha3 NaK subunits in more than half of the medulloblastomas. PMID: 21498719
  44. The increased maternal AChE and Na(+), K(+)-ATPase activities may be due to the low total antioxidant status determined post vaginal delivery, whereas their decreased activities in prematures to their immaturity. PMID: 20964587
  45. Studies indicate that change of Na,K-ATPase activity can influence neurotransmitter release. PMID: 21043236
  46. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
  47. Silencing ATP1A1 inhibits the proliferation of HepG2 cells by decreasing the expression of MAPK1 and induces cell cycle arrest. PMID: 20450619
  48. Results suggest that the association of NHE-1 with Na-K-ATPase is critical for ouabain-mediated regulation of Na-K-ATPase and that these effects may play a role in cardioglycoside-stimulated hypertension. PMID: 20427472
  49. These results suggest that Akt plays a major role in Na(+)/K(+)-ATPase intracellular translocation and thus in alveolar fluid reabsorption. PMID: 20332111
  50. Data conclude that human proximal tubular cells respond to a hyperosmotic challenge with an increase in FXYD2 and Na,K-ATPase protein expression, though to a smaller absolute extent in patient cells. PMID: 19865785
  51. Cardiac glycosides show distinct isoform specific affinities and different affinity profiles to Na(+),K(+)-ATPase isoforms which have different subcellular localizations in human cardiomyocytes. PMID: 19751721
  52. Atp1a1 activity in VSMC and REC cells of rats, dog, and human show that Atp1a1 does not mediate the involvement of ouabain in the development of hypertension in rats, suggesting that the pathogenesis of human and rat hypertension may differ. PMID: 11926353
  53. A search for potential cation binding sites involved calculation of the valence expected from the disposition of oxygen atoms in the models. This identified 3 positions for Na+ & 2 for K+ at which high affinity for the respective cation is expected. PMID: 12461183
  54. hypoxia decreases Na,K-ATPase activity in alveolar epithelial cells by triggering its endocytosis through mitochondrial reactive oxygen species and PKC-zeta-mediated phosphorylation of the Na,K-ATPase alpha(1) subunit PMID: 12671055
  55. data suggest that activity is reduced in the microvillous membrane of placentas from IUGR pregnancies; changes might impair function of Na(+)-coupled transporters and contribute to reduced growth of fetuses PMID: 12788896
  56. sequence 442GDASE446 in the ATP binding pocket of Na/K-ATPase is an important motif and is involved in both the high and low affinity ATP effects rather than in free Mg2+, Na+, and K+ effects PMID: 14522987
  57. At high altitude, the amount of mRNA of Na-K-ATPase, CFTR, and beta-actin of brush biopsies did not change in controls but decreased significantly in high altitude pulmonary edema-susceptible subjects PMID: 14555664
  58. In the control subjects the Na,K-pump isoform alpha1 was increased by 37% in resistance trained compared to untrained leg, and in the diabetics the alpha1 content was 45% higher in resistance trained compared to untrained leg. PMID: 14685860
  59. Decreases in erythrocyte membrane Na(+)K(+) ATPase activity is associated with rheumatoid arthritis PMID: 15515123
  60. N-glycans linked to the beta2 subunit of the Na,K-ATPase contain apical sorting information, and the high abundance of the beta2 subunit isoform along with the absence of the beta1 subunit, is responsible for the unusual apical location of the Na,K-ATPase PMID: 16230337
  61. Mice with the ouabain-sensitive "human-like" alpha1 isoform developed ACTH-induced hypertension to greater extent than WT animals PMID: 16243970
  62. Phosphorylation of AP-2 mu2 subunit is essential for Na+,K+-ATPase endocytosis in response to a variety of signals, such as dopamine or reactive oxygen species. PMID: 16498080
  63. after an ischaemic insult, alpha1 and beta1 protein subunit abundance and mRNA levels are independently regulated. PMID: 16708288
  64. identified a well conserved Na,K-ATPase catalytic alpha-subunit motif that binds to the inositol 1,4,5-trisphosphate receptor and can trigger an anti-apoptotic calcium signal. PMID: 16723354
  65. Na, K ATPase alpha subunit is associated with the Na, K ATPase beta3 subunit on the red blood cells. PMID: 17176442
  66. Elevated Na+ -K+ -ATPase activity postexercise may contribute to reduced fatigue after training. PMID: 17446412
  67. study reports the association of human ATP1A1 (P<0.000005) and Dear (P<0.03) with hypertension in a relatively isolated, case/control hypertension cohort from northern Sardinia PMID: 17446437
  68. 31KKE in H1 and 47HRK in H2 of the Na,K-ATPase have distinct roles in modulating the enzyme's conformational transitions during the catalytic cycle of the enzyme PMID: 17881356
  69. after the 4-wk sprint training period, the expression of the muscle Na+-K+ pump alpha1-subunit and Na+/H+-exchanger isoform 1 was higher PMID: 18094063
  70. Data show that JNK modulates the effect of caspases and NF-kappaB in the TNF-alpha-induced down-regulation of Na+/K+ATPase in HepG2 cells. PMID: 18348163
  71. Ankyrin facilitates intracellular trafficking of alpha1-Na+-K+-ATPase in polarized cells. PMID: 18768923
  72. Moderate apoptotic cell shrinkage may be associated with both an increase in K(+) channel permeability and inhibition of the pump whereas more remarkable shrinkage occurs presumably due to inhibition of the pump. PMID: 18769045
  73. HEXIM1 may participate in tissue-selective determination of glucocorticoid sensitivity via direct interaction with GR at least in certain gene sets including atp1a1 and scnn1a. PMID: 18801933
  74. Significant nominal association with bipolar disooder was observed for single Single Nucleotide Polymorphism(rs11805078 ) in the gene of ATP1A1. PMID: 19058785
  75. FXYD3 silencing prevents proper regulation of Na,K-ATPase, which leads to perturbation of cellular Na+ and K+ homeostasis and changes in the expression of Na,K-ATPase isozymes, whose functional properties are incompatible with cell differentiation. PMID: 19109419
  76. Data show that PLM associates with and modulates both NKA-alpha1 and NKA-alpha2 in a comparable but not identical manner. PMID: 19638348
  77. brain-region specific increases in the activities of Na(+)/K(+)-ATPase and Ca(2+)/Mg(2+)-ATPase in autism PMID: 19863947
  78. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 11509477
  79. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 11887161
  80. Na+-K+--ATPase-mediated signal transduction: from protein interaction to cellular function. PMID: 14993422

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Subcellular Location Cell membrane, sarcolemma, Multi-pass membrane protein, Melanosome
Protein Families Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily
Database Links

HGNC: 799

OMIM: 182310

KEGG: hsa:476

STRING: 9606.ENSP00000295598

UniGene: Hs.371889

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