Code | CSB-EL022599HU |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 84 | ||||
Range % | 80-91 | |||||
1:2 | Average % | 86 | ||||
Range % | 81-92 | |||||
1:4 | Average % | 92 | ||||
Range % | 87-100 | |||||
1:8 | Average % | 95 | ||||
Range % | 88-101 |
The recovery of human spondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 97 | 90-104 | ||||
EDTA plasma (n=4) | 93 | 86-97 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human spondin-1 (SPON1) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human SPON1 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the SPON1 levels in the sample and is measured at 450 nm through a microplate reader.
SPON1 is an extracellular matrix protein that is derived from the floor plate of vertebrate embryos and is involved in the growth of axons during embryonic development, differentiation of neural cells, and the promotion of axonal regeneration post-injury. SPON1 could bind the amyloid precursor protein and inhibit its cleavage by β-secretase, leading to cognitive decline in Alzheimer's disease. Aberrant expression of SPON1 has been found in solid tumors. SPON1 has been reported to promote tumor invasion and metastasis in liver cancer.
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