Human Spondin-1(SPON1) ELISA kit

Code CSB-EL022599HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
spondin 1, extracellular matrix protein
Alternative Names
SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit
Abbreviation
SPON1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %84
Range %80-91
1:2Average %86
Range %81-92
1:4Average %92
Range %87-100
1:8Average %95
Range %88-101
Recovery
The recovery of human spondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9790-104
EDTA plasma (n=4)9386-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.504 2.463 2.484 2.365
52.006 2.042 2.024 1.905
2.51.506 1.479 1.493 1.374
1.250.973 0.897 0.935 0.816
0.6250.511 0.518 0.515 0.396
0.3120.285 0.268 0.276 0.157
0.1560.176 0.187 0.182 0.063
00.121 0.117 0.119  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This human spondin-1 (SPON1) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human SPON1 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the SPON1 levels in the sample and is measured at 450 nm through a microplate reader.

SPON1 is an extracellular matrix protein that is derived from the floor plate of vertebrate embryos and is involved in the growth of axons during embryonic development, differentiation of neural cells, and the promotion of axonal regeneration post-injury. SPON1 could bind the amyloid precursor protein and inhibit its cleavage by β-secretase, leading to cognitive decline in Alzheimer's disease. Aberrant expression of SPON1 has been found in solid tumors. SPON1 has been reported to promote tumor invasion and metastasis in liver cancer.

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Target Background

Function
(From Uniprot)
Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.
Gene References into Functions
  1. gene expression experiment revealed that CYP2B6, SPON1, and GSG1L can be activated concomitantly through a constitutive androstane receptor (CAR) activation pathway PMID: 27010727
  2. SPON1 promotes metastatic progression through Fak and Src dependent pathway in human osteosarcoma. PMID: 26032498
  3. These data suggest that SPON1 may be associated with the differential rate of cognitive decline in Alzheimer disease. PMID: 23535033
  4. High SPON1 expression is associated with high-grade gliomas. PMID: 24158112
  5. Genome-wide scan of healthy human connectome discovers SPON1 gene variant influencing dementia severity PMID: 23471985
  6. downregulates osteoclast recruitment to the root side of periodontal tissue via low-density lipoprotein receptor-related protein 8 and inhibits differentiation of osteoclastic precursors PMID: 21488757
  7. These findings indicate that F-spondin regulates the differentiation of human cementoblast-like cells via BMP7 expression. PMID: 22244873
  8. The unique feature of F-spondin FS domain is the presence of three disulfide bonds associated with the N- and C-termini of the domain and a highly conserved N-linked glycosylation site. PMID: 21569239
  9. expression profile of VSGP/F-spondin identifies this molecule as a potential diagnostic marker or target for developing therapeutic strategies to treat ovarian carcinoma PMID: 16103746
  10. identified F-spondin as a promoting factor for cementoblastic differentiation PMID: 16965763
  11. The crystal structure of the reelin-N domain resolved to 2.0 A reveals a variant immunoglobulin-like fold and potential heparin-binding sites. PMID: 18602404
  12. These studies identify F-spondin as a novel protein in osteoarthritis cartilage, where it may act in situ at lesional areas to activate latent TGF-beta and induce cartilage degradation. PMID: 18780763

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Tissue Specificity
Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.
Database Links

HGNC: 11252

OMIM: 604989

KEGG: hsa:10418

UniGene: Hs.643864

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