Human Spondin-1(SPON1) ELISA kit

Instructions
Code CSB-EL022599HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name spondin 1, extracellular matrix protein
Alternative Names SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit
Abbreviation SPON1
Uniprot No. Q9HCB6
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %84
Range %80-91
1:2Average %86
Range %81-92
1:4Average %92
Range %87-100
1:8Average %95
Range %88-101
Recovery
The recovery of human spondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9790-104
EDTA plasma (n=4)9386-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.504 2.463 2.484 2.365
52.006 2.042 2.024 1.905
2.51.506 1.479 1.493 1.374
1.250.973 0.897 0.935 0.816
0.6250.511 0.518 0.515 0.396
0.3120.285 0.268 0.276 0.157
0.1560.176 0.187 0.182 0.063
00.121 0.117 0.119  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.
Gene References into Functions
  1. gene expression experiment revealed that CYP2B6, SPON1, and GSG1L can be activated concomitantly through a constitutive androstane receptor (CAR) activation pathway PMID: 27010727
  2. SPON1 promotes metastatic progression through Fak and Src dependent pathway in human osteosarcoma. PMID: 26032498
  3. These data suggest that SPON1 may be associated with the differential rate of cognitive decline in Alzheimer disease. PMID: 23535033
  4. High SPON1 expression is associated with high-grade gliomas. PMID: 24158112
  5. Genome-wide scan of healthy human connectome discovers SPON1 gene variant influencing dementia severity PMID: 23471985
  6. downregulates osteoclast recruitment to the root side of periodontal tissue via low-density lipoprotein receptor-related protein 8 and inhibits differentiation of osteoclastic precursors PMID: 21488757
  7. These findings indicate that F-spondin regulates the differentiation of human cementoblast-like cells via BMP7 expression. PMID: 22244873
  8. The unique feature of F-spondin FS domain is the presence of three disulfide bonds associated with the N- and C-termini of the domain and a highly conserved N-linked glycosylation site. PMID: 21569239
  9. expression profile of VSGP/F-spondin identifies this molecule as a potential diagnostic marker or target for developing therapeutic strategies to treat ovarian carcinoma PMID: 16103746
  10. identified F-spondin as a promoting factor for cementoblastic differentiation PMID: 16965763
  11. The crystal structure of the reelin-N domain resolved to 2.0 A reveals a variant immunoglobulin-like fold and potential heparin-binding sites. PMID: 18602404
  12. These studies identify F-spondin as a novel protein in osteoarthritis cartilage, where it may act in situ at lesional areas to activate latent TGF-beta and induce cartilage degradation. PMID: 18780763

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Subcellular Location Secreted, extracellular space, extracellular matrix
Tissue Specificity Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.
Database Links

HGNC: 11252

OMIM: 604989

KEGG: hsa:10418

UniGene: Hs.643864

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