Human Spondin-1(SPON1) ELISA kit

Product Details

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Target Background

Product Details

Target Name

spondin 1, extracellular matrix protein

Associated names

SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit

Abbreviation

SPON1

Uniprot No.

Q9HCB6

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312ng/mL-20ng/mL

Sensitivity

0.156ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SPON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-99
1:2	Average %	101
	Range %	94-108
1:4	Average %	98
	Range %	91-105
1:8	Average %	90
	Range %	85-95

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
20	3.068	2.934	3.001	2.840
10	2.078	1.967	2.022	1.862
5	1.183	1.128	1.155	0.995
2.5	0.666	0.641	0.654	0.493
1.25	0.449	0.431	0.440	0.280
0.625	0.305	0.293	0.299	0.138
0.312	0.228	0.217	0.223	0.062
0	0.164	0.157	0.161

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

Description

This ELISA kit provides quantitative measurement of Spondin-1 (SPON1) in human samples, supporting neuroscience research into this extracellular matrix glycoprotein involved in axon guidance, neuronal development, and emerging roles in Alzheimer's disease pathology. Because SPON1 functions at the intersection of amyloid precursor protein processing and neurodevelopmental signaling, researchers need precise quantification across both circulating and tissue-level compartments.

Detection Range: The 0.312–20 ng/mL range aligns with reported SPON1 concentrations in human plasma and tissue lysates, covering both baseline physiological levels and the elevated expression observed in neurodegenerative contexts. Most serum and plasma samples can be assayed neat or with minimal dilution, simplifying workflow planning.

Sensitivity: At 0.156 ng/mL, the kit detects SPON1 in low-abundance conditions such as cerebrospinal fluid-adjacent fractions, conditioned media from primary neuronal cultures, or samples from early-stage neurodegeneration where subtle expression changes carry biological significance.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables researchers to correlate circulating SPON1 levels with localized expression in brain tissue within the same study, bridging biomarker discovery and mechanistic investigation.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate limited-volume specimens typical of paired CSF-plasma biobanking studies and primary neuron culture experiments.

This kit is directly applicable to Alzheimer's disease biomarker research, axon guidance pathway studies, and neurodevelopmental disorder investigations.

Code	CSB-EL022599HU-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.