Human Statherin(STATH) ELISA kit

Code CSB-EL022817HU
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
statherin
Alternative Names
STAT_HUMAN ELISA Kit; STATH ELISA Kit; Statherin ELISA Kit; Statherin precursor ELISA Kit; STR ELISA Kit
Abbreviation
STATH
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, saliva
Detection Range
78 ng/mL-5000 ng/mL
Sensitivity
19.5 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human STATH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %87-93
1:2Average %95
Range %91-104
1:4Average %86
Range %82-92
1:8Average %96
Range %92-101
Recovery
The recovery of human STATH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9994-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
50002.751 2.651 2.701 2.547
25001.829 1.729 1.779 1.625
12501.087 1.067 1.077 0.923
6250.589 0.579 0.584 0.430
312.50.382 0.372 0.377 0.223
1560.265 0.255 0.260 0.106
780.209 0.207 0.208 0.054
00.154 0.153 0.154  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human STATH ELISA Kit was designed for the quantitative measurement of Human STATH protein in serum, plasma, tissue homogenates, saliva. It is a Sandwich ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL.

Citations

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Target Background

Function
(From Uniprot)
Salivary protein that stabilizes saliva supersaturated with calcium salts by inhibiting the precipitation of calcium phosphate salts. It also modulates hydroxyapatite crystal formation on the tooth surface.
Gene References into Functions
  1. The total protein and statherin in the in-vivo AEP were different between eroded and non-eroded tooth surfaces of the same patient PMID: 28837608
  2. effects of statherin on intracellular calcium level and its subsequent related molecular alterations would give us new pathogenic aspect in oral carcinogenesis PMID: 25128293
  3. phenylalanine orientations in statherin bound to hydroxyapatite surfaces PMID: 22563672
  4. Data show that the full characterization of the statherin peptides generated facilitates the elucidation of their novel functional roles in the oral and gastro-intestinal environment. PMID: 20731414
  5. insight into the molecular interactions of statherin with hydroxyapatite surfaces PMID: 19678690
  6. inhibits calcium phosphate precipitation PMID: 12060866
  7. results suggest that a layer rich in statherin forms at the interface of saliva and air, and that the surface rheology developed is dependent upon protein interactions mediated by calcium PMID: 15769251
  8. In conclusion, statherin induces transition to yeast of Candida albicans hyphae and may thus contribute to the oral defense against candidiasis. PMID: 19799638

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Subcellular Location
Secreted.
Protein Families
Histatin/statherin family
Tissue Specificity
Secreted by parotid and submandibular glands.
Database Links

HGNC: 11369

OMIM: 184470

KEGG: hsa:6779

STRING: 9606.ENSP00000246895

UniGene: Hs.654495

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