Human Sterol regulatory element-binding protein 1(SREBF1) ELISA kit

Instructions
Code CSB-EL022657HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name sterol regulatory element binding transcription factor 1
Alternative Names SREBP-1c, SREBP1, bHLHd1, sterol regulatory element binding protein-1
Abbreviation SREBF1
Uniprot No. P36956
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SREBF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 88-99
1:2 Average % 90
Range % 85-97
1:4 Average % 94
Range % 90-100
1:8 Average % 94
Range % 86-102
Recovery
The recovery of human SREBF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 91-98
EDTA plasma (n=4) 97 92-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 2.617 2.658 2.638 2.532
250 2.246 2.273 2.260 2.154
125 1.786 1.790 1.788 1.682
62.5 1.175 1.199 1.187 1.081
31.2 0.638 0.639 0.639 0.533
15.6 0.369 0.355 0.362 0.256
7.8 0.196 0.207 0.202 0.096
0 0.105 0.106 0.106  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
Subcellular Location Endoplasmic reticulum membrane, Multi-pass membrane protein, Golgi apparatus membrane, Multi-pass membrane protein, Cytoplasmic vesicle, COPII-coated vesicle membrane, Multi-pass membrane protein
Protein Families SREBP family
Tissue Specificity Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma
Database Links

HGNC: 11289

OMIM: 184756

KEGG: hsa:6720

STRING: 9606.ENSP00000348069

UniGene: Hs.592123

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