Human T-complex protein 1 subunit zeta(CCT6A) ELISA kit

Code CSB-EL004865HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chaperonin containing TCP1, subunit 6A (zeta 1)
Alternative Names CCT6A ELISA Kit; CCT6 ELISA Kit; CCTZ ELISA Kit; T-complex protein 1 subunit zeta ELISA Kit; TCP-1-zeta ELISA Kit; Acute morphine dependence-related protein 2 ELISA Kit; CCT-zeta-1 ELISA Kit; HTR3 ELISA Kit; Tcp20 ELISA Kit
Abbreviation CCT6A
Uniprot No. P40227
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CCT6A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %84-95
1:2Average %94
Range %88-98
1:4Average %92
Range %83-97
1:8Average %98
Range %94-102
Recovery
The recovery of human CCT6A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9894-103
EDTA plasma (n=4)8580-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.561 2.445 2.503 2.371
8002.115 2.017 2.066 1.934
4001.382 1.424 1.403 1.271
2000.741 0.736 0.739 0.607
1000.422 0.414 0.418 0.286
500.264 0.255 0.260 0.128
250.191 0.197 0.194 0.062
00.133 0.131 0.132
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Gene References into Functions
  1. High expression of CCT6A is associated with Non-Small Cell Lung Cancer. PMID: 28104536
  2. selective inhibition of SMAD3 or CCT6A efficiently suppresses TGF-beta-mediated metastasis. Findings provide a mechanism that directs TGF-beta signaling toward its prometastatic arm and may contribute to the development of therapeutic strategies targeting TGF-beta for non-small-cell lung carcinoma. PMID: 28375158
  3. These data suggest that CCT6A is a novel autoantigen recognized by Vdelta2 gammadelta T cells, which deepens our understanding of mechanisms in autoimmune diseases. PMID: 27489109
  4. identified genes, which include Tcp20, may play important role in conferring radioresistance to oral squamous cell carcinoma and could be useful in identifying cases with more radioresistance PMID: 16211299
Subcellular Location Cytoplasm.
Protein Families TCP-1 chaperonin family
Database Links

HGNC: 1620

OMIM: 104613

KEGG: hsa:908

STRING: 9606.ENSP00000275603

UniGene: Hs.82916

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