Human Tenascin-X(TNXB) ELISA kit

Code CSB-EL024036HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
tenascin XB
Alternative Names
EDS3 ELISA Kit; Ehlers Danlos like syndrome ELISA Kit; Growth inhibiting protein 45 ELISA Kit; Hexabrachion like protein ELISA Kit; Hexabrachion-like protein ELISA Kit; HXBL ELISA Kit; NXB2 ELISA Kit; Tenascin X ELISA Kit; Tenascin X precursor ELISA Kit; Tenascin XB ELISA Kit; Tenascin XB1 ELISA Kit; Tenascin XB2 ELISA Kit; Tenascin-X ELISA Kit; TENX ELISA Kit; TENX_HUMAN ELISA Kit; TN X ELISA Kit; TN-X ELISA Kit; TNX ELISA Kit; TNXB ELISA Kit; TNXB1 ELISA Kit; TNXB2 ELISA Kit; TNXBS ELISA Kit; VUR8 ELISA Kit; XB ELISA Kit; XBS ELISA Kit
Abbreviation
TNXB
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
46.88 ng/mL-3000 ng/mL
Sensitivity
11.7 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNXB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 85-97
1:2 Average % 101
Range % 95-105
1:4 Average % 92
Range % 84-97
1:8 Average % 100
Range % 92-105
Recovery
The recovery of human TNXB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 85-97
EDTA plasma (n=4) 94 88-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
3000 1.991 1.934 1.963 1.816
1500 1.609 1.576 1.593 1.446
750 1.230 1.200 1.215 1.068
375 0.732 0.712 0.722 0.575
187.5 0.530 0.507 0.519 0.372
93.75 0.369 0.355 0.362 0.215
46.88 0.261 0.257 0.259 0.112
0 0.149 0.145 0.147  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TNXB ELISA Kit was designed for the quantitative measurement of Human TNXB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 ng/mL-3000 ng/mL and the sensitivity is 11.7 ng/mL.

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Target Background

Function
(From Uniprot)
Appears to mediate interactions between cells and the extracellular matrix. Substrate-adhesion molecule that appears to inhibit cell migration. Accelerates collagen fibril formation. May play a role in supporting the growth of epithelial tumors.
Gene References into Functions
  1. mRNA for tenascin-X gene values was higher in ventricular septal defects. PMID: 29470764
  2. Hypermethylated sites at TNXB are associagted with response to starvation in anorexia nervosa. PMID: 27367046
  3. Study describes a biallelic TNXB variants in patients with congenital adrenal hyperplasia due to CYP21A2 deletions resulting in a classical Ehlers-Danlos syndrome phenotype with skin hyperextensibility, widened atrophic scars and joint hypermobility. PMID: 27297501
  4. patients with the TNX-deficient type EDS typically have generalized joint hypermobility, skin hyperextensibility and easy bruising. In contrast to the classical type, the inheritance pattern is autosomal recessive and atrophic scarring is absent. Molecular analysis of TNXB in a diagnostic setting is challenging. PMID: 27582382
  5. the identification of a rare missense variant in TNXB in combination with a positive family history of VUR and joint hypermobility may represent a non-invasive method to diagnose PVUR and warrants further evaluation in other cohorts PMID: 26408188
  6. We then quantified the tenascin-X level in serum of patients and identified tenascin-X as potent marker for ovarian cancer, showing that secretomic analysis is suitable for the identification of protein biomarkers when combined with protein immunoassay. PMID: 26090390
  7. It plays regulatory roles in collagen functions such as fibril organization and fibrillogenesis in calcific aortic valves. PMID: 25926574
  8. these results suggest that mutations in TNXB can cause hereditary primary vesicoureteral reflux . PMID: 23620400
  9. Noticeable decreased expression of tenascin-X in calcific aortic valves. PMID: 22827484
  10. Tenascin-X haploinsufficiency was associated with Ehlers-Danlos syndrome in patients with congenital adrenal hyperplasia PMID: 23284009
  11. no difference in genotype frequency was detected between patients who experienced a re-dislocation after the initial surgery and patients who did not sustain a re-dislocation. PMID: 22991340
  12. Genome-wide association study of age-related macular degeneration identifies TNXB, FKBPL and NOTCH4 as candidate susceptibility genes. PMID: 22694956
  13. Combined analysis of tenascin-C expression and the nodule size improved the prediction of malignancy in this patient cohort. PMID: 22588153
  14. rs204887 itself or a nearby variant is unlikely to play a major role in the development of schizophrenia although a cumulative contribution of rare variants in the TNXB gene cannot be ruled out. PMID: 21317684
  15. Three point mutations in TNX gene were found to be associated with hypermobility type Ehlers-Danlos syndrome (EDS) . The phenotypic effects of V1195M mutation on 7th fibronectin Type III domain (TNXfn7) with regards to EDS were investigated. PMID: 20853426
  16. localization and analysis of the principal promoter for human tenascin-X PMID: 12376099
  17. chromosomal mapping of a de novo unequal crossover causing a deletion of the steroid 21-hydroxylase gene and a non-functional hybrid tenascin-X gene PMID: 12746407
  18. The transmission disequilibrium test did not show allelic association between these two TNXB single nucleotide polymorphisms and schizophrenia, and the rs1009382-rs204887 haplotypes were not associated with the illness either. PMID: 14729256
  19. Both elastic fiber abnormalities and reduced collagen content contribute to the observed phenotype in TNX-deficient patients. PMID: 15102077
  20. different distributions of tenascin-C and -X were found around the epithelium and the endomysium of the mental symphyseal region, and affect the specific formation of the mandible during ossification in the fetus PMID: 15455729
  21. elastic fiber abnormalities in hypermobility type Ehlers-Danlos syndrome are specific for TNX-haploinsufficient individuals and confirm an important role for TNX in regulating elastic fiber integrity PMID: 15733269
  22. Tenascin-X expression is markedly decreased in AAA tissue, and AAA is associated with high serum concentrations of tenascin-X. PMID: 16567571
  23. TNX contributes to matrix stability and is possibly involved in collagen fibril formation. PMID: 17033827
  24. Association of the TNXB locus or its adjacent region of the NOTCH4 locus with schizophrenia. PMID: 17192952
  25. TNXB and TNC may be involved in the malignant transformation of plexiform neurofibromas PMID: 17202312
  26. Multiple species of TNX in blood were identified and characterized. PMID: 17263730
  27. TNX is unlikely to be involved in matrix deposition in the early phase of wound healing, but it is required in the later phase when remodeling and maturation of the matrix establishes and improves its biomechanical properties. PMID: 17453911
  28. TNXB(tenascin XB protein) gene is a candidate gene susceptible to Systemic lupus erythematosus in the Japanese population. PMID: 18058064
  29. This study showed different patterns of expression of tenascin and fibronectin along the process of tumorigenesis and tumor progression in pleomorphic adenoma, a fact that might play a role in invasion properties of these tumors. PMID: 18091320
  30. Data indicate a complex architecture of the extracellular matrix in the uterosacral ligaments, with marked differences in tenascin and elastin expression between postmenopausal women with or without pelvic organ prolapse. PMID: 18155129
  31. TNX-deficient women are at risk of obstetric complications. PMID: 18335242
  32. These results suggest possible involvement of XB-S in the function of Eg5. PMID: 18679583
  33. Tenascin-X may be a new diagnostic marker of malignant mesothelioma in the differential diagnosis of cancers involving the serosal cavities. PMID: 19738457

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Involvement in disease
Ehlers-Danlos syndrome due to tenascin X deficiency (EDSTNXD); Vesicoureteral reflux 8 (VUR8)
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Tenascin family
Tissue Specificity
Highly expressed in fetal adrenal, in fetal testis, fetal smooth, striated and cardiac muscle. Isoform XB-short is only expressed in the adrenal gland.
Database Links

HGNC: 11976

OMIM: 600985

KEGG: hsa:7148

STRING: 9606.ENSP00000407685

UniGene: Hs.485104

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