Product Details

Target Name

triggering receptor expressed on myeloid cells-like 1

Alternative Names

dJ238O23.3 ELISA Kit; GLTL1825 ELISA Kit; MGC119173 ELISA Kit; PRO3438 ELISA Kit; TLT 1 ELISA Kit; TLT-1 ELISA Kit; TLT1 ELISA Kit; TREM like transcript 1 ELISA Kit; Trem like transcript 1 protein ELISA Kit; Trem-like transcript 1 protein ELISA Kit; TREML 1 ELISA Kit; Treml1 ELISA Kit; Triggering receptor expressed on myeloid cells like 1 ELISA Kit; Triggering receptor expressed on myeloid cells like protein 1 ELISA Kit; Triggering receptor expressed on myeloid cells-like protein 1 ELISA Kit; TRML1_HUMAN ELISA Kit

Abbreviation

TREML1

Uniprot No.

Q86YW5

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TREML1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-94
1:2	Average %	100
	Range %	95-104
1:4	Average %	97
	Range %	86-102
1:8	Average %	102
	Range %	94-107

Recovery

The recovery of human TREML1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-97
EDTA plasma (n=4)	96	87-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.396	2.304	2.350	2.196
2000	2.017	1.976	1.997	1.843
1000	1.536	1.500	1.518	1.364
500	1.006	0.995	1.001	0.847
250	0.569	0.551	0.560	0.406
125	0.355	0.349	0.352	0.198
62.5	0.275	0.266	0.271	0.117
0	0.151	0.157	0.154

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human TREML1 ELISA Kit was designed for the quantitative measurement of Human TREML1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Target Background

Function
(From Uniprot)

Cell surface receptor that may play a role in the innate and adaptive immune response.

Gene References into Functions

Study characterized the expression of TREM genes in cerebellum and temporal cortex and determined TREML1 and TREM2 to be the only reliably expressed TREM genes in these brain regions; identified rs9357347 as a putative regulatory variant that is associated with protection from Alzheimer's disease and with increased TREML1 and TREM2 brain levels. PMID: 27939925
Levels of sTLT-1 in plasma from patients with potential acute coronary syndrome were compared with an age-matched control group with similar risk factors for cardiovascular disease. PMID: 25147325
Platelet-derived soluble TLT-1 is a potent endogenous regulator of sepsis-associated inflammation. PMID: 22551551
Serum sTREM-1 level measurements may be helpful in diagnosing and predicting outcome for non-tuberculous mycobacterial lung disease. PMID: 22283904
These results suggest that during inflammation, sTLT-1 may mediate hemostasis by enhancing actin polymerization, resulting in increased platelet aggregation and adherence to the endothelium. PMID: 20093931
initial characterization of TREM-like transcript (TLT)-1 results indicate it is likely that TLT-1 regulates the signaling of the TREM family receptors(TLT-1) PMID: 12393607
In contrast to previously characterized immunoreceptor tyrosine-based inhibition motif (ITIM) receptors, TLT1 enhances rather than inhibits calcium signaling in rat RBL-2H3 cells, a property dependent on the SHP-2 recruiting classical Tyr281 ITIM. PMID: 15128762
A soluble fragment of the TLT-1 extracellular domain is found in serum of humans and mice and that an isoform of similar mass is released from platelets following activation with thrombin. PMID: 16505478
Define a platelet specific function for TLT1 in regulation of aggregation. PMID: 17549298
Phenotypic heterogeneity in the Gray platelet syndrome extends to the expression of TREM family member, TLT-1. PMID: 18612537
antibody against the extracellular domain inhibits platelet aggregation in vitro PMID: 19230638
TLT-1 plays a protective role during inflammation by dampening the inflammatory response and facilitating platelet aggregation at sites of vascular injury PMID: 19436112

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Sequestered in cytoplasmic vesicles in resting platelets (PubMed:15100151). Transported to the cell surface after stimulation by thrombin (PubMed:15100151). Soluble fragments can be released into the serum by proteolysis (PubMed:16505478).

Tissue Specificity

Detected in platelets, monocytic leukemia and in T-cell leukemia.

Database Links

HGNC: 20434

OMIM: 609714

KEGG: hsa:340205

STRING: 9606.ENSP00000402855

UniGene: Hs.117331

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Code	CSB-EL024406HU
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Human Trem-like transcript 1 protein(TREML1) ELISA kit

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