Human Vasoactive Intestinal Peptide,VIP ELISA Kit

Instructions
Code CSB-E08354h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name vasoactive intestinal peptide
Alternative Names Peptide histidine methioninamide 27 ELISA Kit; Peptide histidine valine 42 ELISA Kit; PHM27 ELISA Kit; PHV42 ELISA Kit; Prepro VIP ELISA Kit; Vasoactive intestinal peptide ELISA Kit; Vasoactive intestinal polypeptide ELISA Kit; VIP ELISA Kit; VIP peptides ELISA Kit; VIP peptides precursor ELISA Kit; VIP_HUMAN ELISA Kit
Abbreviation VIP
Uniprot No. P01282
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %80-95
1:2Average %95
Range %91-99
1:4Average %99
Range %92-105
1:8Average %92
Range %86-100
Recovery
The recovery of human VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9989-105
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.795 2.598 2.697 2.547
5002.320 2.189 2.255 2.105
2501.644 1.564 1.604 1.454
1250.913 0.885 0.899 0.749
62.50.425 0.416 0.421 0.271
31.250.289 0.281 0.285 0.135
15.60.227 0.224 0.226 0.076
00.151 0.149 0.150  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
Gene References into Functions
  1. VIP SNPs are associated with VIP serum level and response to rheumatoid arthritis treatment. PMID: 29391448
  2. this review provides an overview of VIP effects on rheumatoid arthritis pathology PMID: 30155495
  3. Results have suggested that pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP), but predominantly VIP, induce neuroblastoma differentiation into benign form by regulating hypoxia-inducible factors, vascular endothelial growth factor and vascular endothelial growth factor receptor expression and distribution. PMID: 29699729
  4. Results found that VIP enhanced trophoblast cell migration and invasion through the activation of high affinity VPAC receptors and PKA-CRE signaling pathways suggesting that VIP-mediated autocrine pathways regulate trophoblast cell function and contribute to immune homeostasis maintenance at placentation. PMID: 27212399
  5. this study shows that that VIP exerts anti-inflammatory effects by inhibiting the MAPK-p47phox phosphorylation-NOX2 activation axis, and that VIP dampens carrageenan-induced inflammation in rats PMID: 27271317
  6. Both mast cells and VIP appear to be important factors in the regulation of this bacterial translocation through the colonic mucosa of irritable bowel syndrome patients. PMID: 28711627
  7. we found that VIP inhibits NFAT nuclear translocation in primary human pulmonary artery smooth muscle cells (PASMC). Early activation of NFATc3 in IPF patients may contribute to disease progression and the increase in VIP expression could be a protective compensatory mechanism PMID: 28125639
  8. VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts. PMID: 26818776
  9. optogenetically activating single VIPs in live mice while recording the activity of nearby neurons, we find that VIPs break open a hole in blanket inhibition. PMID: 27013676
  10. a differential effect of VIP on the expression of TNFalpha and IL-6 receptors, since VIP was only able to decreased expression in LPS-stimulated monocytes but not in 4/74-infected monocytes. PMID: 26206287
  11. VIP promotes Th17 polarization of memory Th cells in early arthritis. PMID: 25957307
  12. The circadian rhythm amplitude of motor activity in both Alzheimer disease subjects and age-matched controls is correlated with the number of vasoactive intestinal peptide-expressing suprachiasmatic nucleus neurons. PMID: 25921596
  13. Skin VIP expression, a marker of sudomotor innervation, was significantly lower in patients with familial transthyretin amyloid neuropathy compared to controls. PMID: 25973863
  14. Alterations in VIP expression may play a role in irritable bowel syndrome. PMID: 25574088
  15. Interictal CGRP and, to a lesser degree, VIP levels measured in peripheral blood are of great help in predicting response of chronic migraine patients to Onabotulinumtoxin type A PMID: 24673487
  16. The antimicrobial peptide, VIP is a neuropeptide with activity against a range of microorganisms from skin, oral, respiratory and gastrointestinal tract sites. PMID: 18603306
  17. VIP is significantly increased in the patients with chronic obstructive pulmonary disease and pulmonary hypertension. PMID: 24228853
  18. Serum levels of vasoactive intestinal peptide as a prognostic marker in early arthritis. PMID: 24409325
  19. Data suggest that two closely related neuroprotective peptides, vasoactive intestinal peptide (VIP) and pituitary adenylyl cyclase-activating peptide (PACAP), are up-regulated in neurons/immune cells after injury and/or inflammation. [REVIEW] PMID: 23517078
  20. Our study suggested that baicalein exerts a positive effect on the VIP expression in HCMV-infected EVT at maternal-fetal interface. PMID: 23771668
  21. VIP can inhibit the cytotoxicity of natural killer cells to MKN45 cells and can inhibit the expressions of NKG2D, DAP10, and NF-kappaB in natural killer cells. PMID: 24228003
  22. The results of this study indicate a connection between elevated concentration of NPY and VIP and decreased DPP IV/CD26 activity and concentration, suggesting a potential role of these molecules in the immunomodulation of rheumatoid arthritis. PMID: 23864142
  23. VIP induced expression of CCL5, one of the main chemokines involved in T cell recruitment. PMID: 24492467
  24. VIP expression and its receptors' polymorphism may be important in determining susceptibility to progression from mild to severe forms of Chagas disease. PMID: 23800435
  25. Expression and distribution of phosphodiesterase (PDE) type 1, known to hydrolyze both cyclic AMP and -GMP, are evaluated with VIP in the vagina where their signaling pathways are involved in control of vaginal blood flow. PMID: 23490535
  26. The present study found that reduced VIP innervation in the vaginal epithelium of the perimenopausal women was correlated with the severity of pelvic organ prolapse with or without stress incontinence. PMID: 23915857
  27. VIP and pituitary adenylate cyclase-activating polypeptide (PACAP) may influence the course of multiple sclerosis. PMID: 24041830
  28. Either PACAP or VIP were able to recover the retinal pigment epithelial barrier hyperpermeability and the reduced transepithelial electrical resistance. PMID: 23220033
  29. VIP in synovial fluid and articular cartilage is negatively associated with progressive joint damage in osteoarthritis and is a potential indictor of disease severity. PMID: 22959974
  30. Confocal images and FACS demonstrated a strong association and co-localization between VIP peptide and CRTH2 molecules PMID: 23168411
  31. An imbalance between vasoactive intestinal peptide and substance P production results in the reestablishment and maintenance of the inflammatory process, leading to denervation and, consequently, promoting the development of megaesophagus. PMID: 22995328
  32. A set of cryosections revealed extensive colocalization of calretinin with vasoactive intestinal peptide in both surviving submucosal perikarya and mucosal nerve fibres in human chagasic submucosa and mucosa. PMID: 22555304
  33. The present work was undertaken to investigate the antiproliferative potential of vasoactive intestinal peptide (VIP) exposure on non-tumoral (HK2) and tumoral (A498, cRCC) human proximal tubular epithelial cell lines. PMID: 22728770
  34. Vasoactive intestinal polypeptipe and GRIN3A SNPs may be associated with cerebral palsy at age 2 in children born preterm. PMID: 22914463
  35. results strongly suggest that VIP synergistically enhances TNF-alpha-stimulated IL-6 and IL-8 synthesis via activating the NF-kappa-B pathway in HK-2 cells PMID: 22207455
  36. We hypothesized that VIP plays a role in the activation of parasympathetic cranial outflow during migraine attacks PMID: 22108610
  37. Physical exercise stimulates production of natural anti-VIP antibodies and likely results in suppression of VIP. PMID: 22140573
  38. One year after sigmoid colon vaginoplasty, VIP expression was significantly lower in the neovagina than in the intact sigmoid colon, but increased 3 years postoperatively. PMID: 21426768
  39. Blood levels of vasoactive intestinal polypeptide tended to be high in patients with ankylosing spondylitis as compared with those of healthy subjects and correlated with platelet counts significantly, for the first time at the literature. PMID: 20340024
  40. Results demonstrate that meibomian gland epithelial transformed cells transcribe and translate VIP and mACh receptors. PMID: 21969302
  41. The VIP level of the nasal septum in the perennial allergic rhinitis patients was higher than that in the normal controls. PMID: 19685709
  42. Substance P and VIP neurofibers are present in the fibroid pseudocapsule of uterine fibroids, similar to the values in the normal myometrium of a non-pregnant uterus. PMID: 21167238
  43. SP, CGRP and VIP may play important roles in the pathophysiological mechanism of vasomotor rhinitis. PMID: 17087112
  44. SP levels are higher in repeat cesarean section, while the VIP levels are reduced in the lower uterine segment. PMID: 20692311
  45. VIP increases its own intracellular and extracellular levels, and could be involved in the regulation of VPAC(1)-receptor traffic from the plasma membrane to the nucleus. PMID: 20691743
  46. Data show that 1/3 of pediatric slow-transit constipation patients have low substance P or vasoactive intestinal peptide nerve fiber density in proximal colon. PMID: 20529207
  47. Data describe neurons and nerve fibers of intestinal plexuses containing vasoactive intestinal peptide, pituitary adenylate cyclase-activating polypeptide and neuropeptide Y in pathologically changed parts of the large intestine in intestinal cancer. PMID: 20675276
  48. Data show that TG2-L and TG2-S enhanced neuroblastoma cell differentiation due to N-Myc small interfering RNA, and suppression of vasoactive intestinal peptide (VIP) expression alone induced neuroblastoma cell differentiation. PMID: 20007697
  49. Besides their presence and functions in the gut and the brain VIP and PACAP have distinct physiological roles in the genital tract. PMID: 11713978
  50. Circulating VIP in fulminant hepatic failure seems to reflect hemodynamic changes in the systemic rather than in the cerebral circulation. PMID: 11884208

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Subcellular Location Secreted
Protein Families Glucagon family
Database Links

HGNC: 12693

OMIM: 192320

KEGG: hsa:7432

STRING: 9606.ENSP00000356213

UniGene: Hs.53973

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