Vitamin B12, also known as cobalamin, is an essential water-soluble vitamin that plays a key role in DNA synthesis, red blood cell formation, and neurological function. This vitamin is unique among B vitamins as it contains cobalt and requires intrinsic factor for absorption in the small intestine. Deficiency of vitamin B12 can lead to megaloblastic anemia and neurological disorders, making its measurement important in clinical diagnostics and nutritional research. The vitamin is primarily obtained from animal products and is stored in the liver for extended periods.
The Human Vitamin B12 ELISA Kit (CSB-E07903h) is designed for quantitative measurement of VB12 in human serum and plasma samples. This competitive assay operates within a detection range of 1.56 pg/mL to 100 pg/mL with a sensitivity of 1.498 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for vitamin B12 analysis in human specimens.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in clinical research to measure vitamin B12 levels in human serum samples from patients with pancreatic neuroendocrine neoplasms, supporting studies of metabolic biomarkers associated with metastatic disease progression. The kit has also been applied in nutritional and metabolic studies examining vitamin B12 status alongside other B-vitamins and homocysteine levels in clinical populations. Additionally, researchers have used this assay in cellular studies to monitor vitamin B12 levels in cell culture systems studying oxidative stress and glutathione metabolism.
• Oncology research: Measurement of vitamin B12 levels in serum samples from pancreatic neuroendocrine tumor patients to study potential biomarker associations with metastatic disease status
• Nutritional metabolism studies: Analysis of vitamin B12 status in clinical populations as part of comprehensive B-vitamin and homocysteine profiling to determine metabolic health parameters
• Cellular metabolism research: Quantification of vitamin B12 levels in cell culture supernatants and cellular extracts during studies examining oxidative stress responses and glutathione metabolism pathways
