Human Vitamin B12,VB12 ELISA Kit

Code CSB-E07903h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Vitamin B12,VB12
Abbreviation
VB12
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
1.56 pg/mL-100 pg/mL
Sensitivity
1.498 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of human VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Materials provided
  • A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human VB12 antibody.
  • Standard (2 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • Biotin-conjugated VB12(100 x concentrate) (1 x 600 μl)
  • HRP-avidin (100 x concentrate) (1 x 120 μl)
  • Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated VB12
  • HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin solution.
  • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
  • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
  • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human VB12 ELISA Kit was designed for the quantitative measurement of Human VB12 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 1.498 pg/mL.

Customer Reviews and Q&A

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 Q&A
Q:

Protocol P9, step 3 - Set a Blank well without any solution.
This is a completely empty well with no solution or liquid at all? Am I correct?
May I know if this is S0. If it is S0, do we add the wash buffer, TMB and stop solution?

A:
Thanks for your inquiry.
Actually step3 is just only one procedure during the whole experiment. You need to check the full steps according our manual.
This kit is competitive one and the blank is different from S0 indeed. The blank should be added Wash Buffer+TMB Substrate+Stop Solution.
You could see it from the protocol. Pls read the manual carefully.
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