Goat vitamin B12 (VB12) ELISA kit

Code CSB-EQ028114GO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation VB12
Target Name vitamin B12 (VB12)
Species Capra hircus (Goat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:10 Average % 85
Range % 80-92
1:20 Average % 97
Range % 90-105
1:40 Average % 97
Range % 92-104
1:80 Average % 92
Range % 86-98
Recovery
The recovery of goat VB12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-100
EDTA plasma (n=4) 97 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average  
100 0.145 0.148 0.147  
50 0.221 0.227 0.224  
25 0.396 0.394 0.395  
12.5 0.837 0.815 0.826  
6.25 1.252 1.243 1.248  
3.12 2.018 2.002 2.010  
1.56 2.679 2.641 2.660  
0 2.754 2.716 2.735  
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