Human activated protein C,APC ELISA Kit

Instructions
Code CSB-E09909h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name activated protein C,APC
Alternative Names N/A
Abbreviation APC
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 91  
Range % 87-96  
1:200 Average % 94  
Range % 90-97  
1:400 Average % 97  
Range % 94-103  
1:800 Average % 94  
Range % 90-98  
Recovery
The recovery of human APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-94  
EDTA plasma (n=4) 87 84-92  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.755 2.990 2.873 2.754  
50 2.065 2.201 2.133 2.014  
25 1.359 1.288 1.324 1.205  
12.5 0.853 0.816 0.835 0.716  
6.25 0.470 0.473 0.472 0.353  
3.12 0.325 0.302 0.314 0.195  
1.56 0.184 0.192 0.188 0.069  
0 0.115 0.122 0.119    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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