Human aldehyde dehydrogenase,ALDH ELISA Kit

Code CSB-E11854h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name aldehyde dehydrogenase 2 family (mitochondrial)
Alternative Names Acetaldehyde dehydrogenase 2 ELISA Kit; Aldehyde dehydrogenase 2 family (mitochondrial) ELISA Kit; Aldehyde dehydrogenase 2 family ELISA Kit; Aldehyde dehydrogenase mitochondrial ELISA Kit; Aldehyde dehydrogenase; mitochondrial ELISA Kit; ALDH 2 ELISA Kit; ALDH class 2 ELISA Kit; ALDH E2 ELISA Kit; ALDH-E2 ELISA Kit; Aldh2 ELISA Kit; ALDH2_HUMAN ELISA Kit; ALDHI ELISA Kit; ALDM ELISA Kit; Liver mitochondrial ALDH ELISA Kit; MGC1806 ELISA Kit; Mitochondrial aldehyde dehydrogenase 2 ELISA Kit; MS767 ELISA Kit; Nucleus encoded mitochondrial aldehyde dehydrogenase 2 ELISA Kit
Abbreviation ALDH2
Uniprot No. P05091
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ALDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %81-94
1:2Average %97
Range %92-101
1:4Average %93
Range %87-98
1:8Average %92
Range %88-96
Recovery
The recovery of human ALDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10298-106
EDTA plasma (n=4)8682-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.315 2.442 2.379 2.207
25001.664 1.785 1.725 1.553
12501.134 1.224 1.179 1.007
6250.746 0.764 0.755 0.583
3120.527 0.512 0.520 0.348
1560.351 0.364 0.358 0.186
780.252 0.259 0.256 0.084
00.171 0.172 0.172
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Gene References into Functions
  1. ALDH2 polymorphism is associated with Gastric and esophageal cancer. PMID: 30202044
  2. The use of aldehyde dehydrogenase (ALDH) for isolating and identifying sub-populations of mesenchymal stromal cells (MSCs) with specific gene profile may represent an alternative method to provide solutions for targeted therapeutic applications. PMID: 29333563
  3. The analysis of the effect of the individual SNPs(PON1, IL-6, ITGB3, and ALDH2 ) and GRS groups on different lipid profile parameters revealed no significant association of any of the tested SNPs with any lipid parameter, however, the GRS groups showed marginally significant for TC and highly significant association for TG, LDL-c and HDL-c PMID: 30261890
  4. In a Chinese Han population with dyslipidemia, individuals with A/A or A/G genotypes of rs671 in the Aldehyde Dehydrogenase, Mitochondrial gene have a higher coronary artery disease risk, if they lack physical activity and take fried food regularly, than individuals with G/G genotypes. PMID: 29960587
  5. aldehyde dehydrogenase 2 (ALDH2) is found to be transcriptionally regulated by VHL and contributes to enhanced anthracyclines cytotoxicity in clear cell renal cell carcinoma cells. PMID: 28643803
  6. ardiac-specific expression of the human ALDH2 gene in mice augmented myocardial ALDH2 activity but did not improve cardiac function in response to pressure overload. After 12 weeks of transverse aortic constriction , ALDH2 transgenic mice had larger hearts than their wild-type littermates and lower capillary density PMID: 29885625
  7. Study revealed that the rs10744777 polymorphism in ALDH2 was an independent risk factor for ischemic stroke in Chinese male, but not in Chinese female. PMID: 30077765
  8. ALDH2 gene polymorphism is associated with liver diseases. PMID: 29582627
  9. In the 7,966 individuals from the Hakka population of China in this study, the frequencies of the ALDH2 genotypes *1/*1, *1/*2 and *2/*2 were 52.03%, 39.67%, and 8.30%, respectively; 47.97% of the individuals were found to carry the ALDH2*2 genotype, which was associated with a deficiency in the aldehyde dehydrogenase (ALDH2) enzyme activity. PMID: 29623947
  10. Here the authors demonstrate that a missense single nucleotide polymorphism in the ALDH2 gene, rs671 (ALDH2*2), is significantly associated with hip fracture (odds ratio = 2.48, 95% confidence interval: 1.20-5.10, p = 0.021). The rs671 single nucleotide polymorphism was also significantly associated with osteoporosis development (odds ratio = 2.04, 95% confidence interval: 1.07-3.88, p = 0.040). PMID: 28348376
  11. Three novel SNPs of ALDH2 gene were suggestively significantly associated with serum folate levels. PMID: 29953918
  12. Coronary Artery Disease patients with mutated ALDH2 genotype are inclined to suffer with coronary artery lesions than wild type subjects. PMID: 29877297
  13. he ALDH2 Glu504Lys polymorphism may be associated with susceptibility to colorectal cancer (meta-analysis). PMID: 29026383
  14. Many studies have shown that ALDH2 polymorphism is a genetic instrument for alcohol use in Mendelian randomization analysis. [review] PMID: 26212265
  15. Divers studies provide evidence that ALDH2*2 mutant allele (deficiency genotype) is present in nearly half of the East Asian population with ischemic reperfusion injury. [review] PMID: 27633387
  16. Ethnicity and ALDH2*2 altered the relationship of age of drinking initiation as a risk factor for alcohol use disorder symptoms. PMID: 28847041
  17. Studied the association between aldehyde dehydrogenase 2(ALDH2) polymorphisms, and risk and prognosis of individuals developing esophageal cancer in Xinjiang Kazak and Han populations. PMID: 28464297
  18. The ALDH2 Glu504Lys polymorphism can modify the association between alcohol intake and serum LDL cholesterol in Japanese men. PMID: 29176272
  19. A total of 394 patients with acute cerebral infarction were recruited for ALDH2 genotyping using direct sequencing. ALDH2 gene Glu504Lys was significantly associated with short-term prognosis. Short-term prognosis was assessed in accordance with the modified Ranking Scale (mRS). The genotype GA+AA of ALDH2 gene Glu504Lys locus was an independent risk factor of poor 90-day prognosis. PMID: 28939800
  20. Heavy alcohol drinking down-regulates ALDH2 gene expression level. Heavy smoking up-regulates SOD2 gene expression level in patients with head and neck squamous cell carcinoma. PMID: 28841898
  21. For the first time, the common variant rs2339840 was reported to be a protective factor against stroke in coronary heart disease patients with percutaneous coronary intervention. PMID: 29742731
  22. These results indicated that rs671 of ALDH2 may not associate with the risk of EH. PMID: 28613083
  23. This study aims to explore whether Parkinson's disease (PD) patients with reduced ALDH2 activity owing to the rs671 polymorphism are at risk for neuropsychological impairments. PMID: 27453488
  24. ALDH2 polymorphisms might have different effects on the neuropsychological performance of bipolar-II patients with and without comorbid anxiety disorder PMID: 29425204
  25. The missense variant rs671 in ALDH2 was significantly associated with serum uric acid PMID: 29408531
  26. ALDH2 polymorphisms are significantly associated with the risk of drug addiction in the Chinese Han population. PMID: 28052001
  27. Results suggested that styrene metabolism and styrene-induced genotoxicity could be particularly modified by ALDH2 polymorphisms. The important role of ALDH2 indicated that the accumulation of styrene glycoaldehyde, a possible genotoxic intermediate of styrene. PMID: 27224914
  28. Alcohol consumption and ALDH2 SNP rs671 should be considered simultaneously when assessing the gastric cancer risk in a Korean population. PMID: 28036260
  29. by an integrated analysis of the genotypes and the serum levels of APOA5, BUD13 and triglyceride, we observed that BUD13 was another potential mediator, besides APOA5, of the association between rs651821 and serum triglyceride. rs671 (ALDH2), an east Asian-specific common variant, was found to be associated with MetS (Pcombined = 9.7 x 10(-22) ) in Han Chinese PMID: 28371326
  30. The single nuclear polymorphism rs671 in ALDH2 results in a loss of function and hence a decrease in enzyme activity leading to the accumulation of acetaldehyde. It is found in 30 to 50% of East Asians and is almost exclusively confined to these populations PMID: 27575312
  31. Results show significant decreased risk among carriers of one or both Lys alleles of rs671 for invasive mucinous ovarian cancer and for invasive and borderline mucinous tumors. Because the rs671 Lys allele causes ALDH2 inactivation leading to increased acetaldehyde exposure, the observed inverse association with mucinous ovarian cancer is inferred to mean that alcohol intake may be a risk factor for this histotype. PMID: 29247577
  32. evidence of a stronger effect for ALDH2*2(-) compared to ALDH2*2(+) with greater alcohol use when students were more exposed to peer drinking. PMID: 28471244
  33. observed a suggestive association with delayed platelet engraftment, which warrants further examination. These results may suggest that the recipient ALDH2 genotype affects the metabolism of endogenous aldehydes, leading to a significant impact on transplantation outcomes. PMID: 28389253
  34. Luciferase reporter assays showed functional significance of genotype, methylation and a genotype-epigenotype interaction in alcoholism PMID: 28430929
  35. These data suggest that rs671, a common functional single nucleotide polymorphism of ALDH2, is a genuine gout-associated single nucleotide polymorphism in the MYL2-CUX2 locus and that "A" allele (Lys) of rs671 plays a protective role in the development of gout. PMID: 27181629
  36. Findings suggested that ALDH2 can influence atherosclerotic plaque development and vulnerability, and inflammation via MAPK, NF-kappaB and AP-1 signaling pathways. PMID: 27191745
  37. ULK1 played a crucial role in ALDH2-offered protective effect against high glucose exposure-induced cardiomyocyte injury through regulation of autophagy PMID: 29128638
  38. ALDH2*2 is prevalent (51.0%) among Japanese STEMI patients, and those with ALDH2*2 had higher frequencies of coronary spasm and AFS and more-severe myocardial injury compared to those with ALDH2*1/*1. PMID: 27153870
  39. ALDH2 polymorphism might be a promising prognostic factor for Japanese patients with p16-negative OPC PMID: 29206831
  40. In Japanese alcoholic men, the ALDH2*1/*1 genotype was associated with lower platelet counts for an 8-week hospital stay. PMID: 27991683
  41. This review discusses the unexpected interrelationship between aldehydes, ALDH2 and hematopoietic stem cell biology, and in particular its relationship to Fanconi anemia. PMID: 27650066
  42. All genotypes of ALDH2 showed no apparent difference in vasodilation in vivo nitroglycerin-mediated dilation. PMID: 28342890
  43. ALDH2 polymorphism has several subtle effects on the lungs, some of which are similar to changes observed during normal aging, suggesting a "premature lung aging" effect. PMID: 28431562
  44. there is a significant association between ALDH2 rs671 polymorphism and essential hypertension susceptibility (Meta-Analysis) PMID: 28472173
  45. This exome-wide association study indicated that C12orf51 rs11066280, MYL2 rs12229654, and ALDH2 rs671 polymorphisms are linked to blood Pb levels in the Korean population. PMID: 28212632
  46. The ALDH2 Glu504Lys polymorphism was associated with endothelium-dependent dilation disorders in Chinese Han patients with essential hypertension, providing further evidence that this mutation and 'alcohol flush' are not harmless in this Asian population. PMID: 26691593
  47. ALDH2 rs671 was significantly related with Type 2 Diabetic Mellitus. The ALDH2 rs671 might be able to be used as a predictor for the risk of Type 2 Diabetic Mellitus (Meta-Analysis) PMID: 28208752
  48. ALDH2-catalyzed NO formation is necessary and sufficient for nitroglycerin bioactivation in vascular smooth muscle cells. PMID: 27679490
  49. plays an important role in protecting against hepatocellular carcinoma (HCC) metastasis; forced expression in HCC cells inhibited cell migration and invasion both in vitro and in vivo, at least partly by modulating the ALDH2-ACE redox-AMPK axis PMID: 28027570
  50. We studied the ALDH2 genotypes of 35 Japanese FA patients and their mothers. We found that a normal maternal ALDH2 allele was not essential for fetal development of ALDH2-deficient patients, and none of the post-natal clinical parameters were clearly affected by the maternal ALDH2 genotype in these patients. PMID: 27377053

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Subcellular Location Mitochondrion matrix.
Protein Families Aldehyde dehydrogenase family
Database Links

HGNC: 404

OMIM: 100650

KEGG: hsa:217

STRING: 9606.ENSP00000261733

UniGene: Hs.604551

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