Human anti-Mullerian hormone (AMH) ELISA kit

Instructions
Code CSB-E12756h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The product CSB-E12756h manufactured by CUSABIO is a solid-phase sandwich ELISA kit that allows for the quantitative determination of anti-Mullerian hormone (AMH) in the samples, including serum, plasma, and tissue homogenates. This human AMH ELISA kit specifically recognizes the human AMH. It has been quality validated through multiple criteria, including sensitivity, specificity, precision, and lot-to-lot consistency. More details are described in the product instructions.

Samples and standards are respectively pipetted into the appropriate micro ELISA plate wells pre-coated with anti-human AMH antibody. The Biotin-labeled AMH antibody is added to form a sandwich of immune complexes. HRP-avidin and TMB substrate are successively added, and the solution turns blue. The blue turns yellow after the addition of the stop solution. The color intensity is proportional to the AMH content in the sample.

AMH is a homodimeric glycoprotein belonging to the transforming growth factor-beta family. AMH gets its name because of its role in the regression of the Mullerian ducts during male sex differentiation. AMH is produced by granulosa cells of growing ovarian antral follicles. Serum AMH is the preferred ovarian reserve marker and also can serve as a useful biomarker to indicate the risk of menstrual disturbance due to polycystic ovarian syndrome (PCOS). AMH is regarded as a neuroendocrine regulator of fertility based on AMH receptors outside of the gonads and their ability to regulate both gonadotropic cells of the anterior pituitary and the hypothalamus.

Target Name anti-Mullerian hormone
Alternative Names AMH ELISA Kit; MIF ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
Abbreviation AMH
Uniprot No. P03971
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human AMH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled AMH antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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 Q&A
Q:

Could I ask, is this product a generation II Elisa kit ? If not, could you suggest an alternative ?

A:
Thanks for your inquiry.
You mentioned Generation II , it means anti-Mullerian hormone(AMH)generation II Elisa kit indeed.
And then it is this kit CSB-E12756h. Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
Gene References into Functions
  1. Women with higher testosterone and AMH had more frequent anovulatory cycles but there were marginal impacts on time to pregnancy or pregnancy loss. PMID: 29428315
  2. These findings indicate a pregnancy-associated AMH-decline independent of pre-pregnancy elevated AMH levels. PMID: 30146442
  3. serum AMH levels were negatively correlated with age. AMH concentrations approximately 31.1% depended on age and descended by an average of 6.2% per year. Around 25, 35 and 40 years, the decrease of AMH values accelerated. PMID: 29671678
  4. AMH is a poor independent predictor of live birth outcome in either fresh or frozen embryo transfer. PMID: 29331235
  5. AMH, IGF1 and leptin levels in follicular fluid have no relation to the fertility disorders caused by endometriosis or fallopian tube damage, though they are biomarkers for anovulatory fertility disorders. PMID: 29595066
  6. serum levels between women with insulin resistance and without insulin resistance in polycystic ovary syndrome were not significantly different PMID: 29605710
  7. Data suggest that serum levels of AMH are down-regulated during second half of gestation in (1) women with gestational diabetes, (2) pregnant women with type 2 diabetes, and (3) control pregnant women. A positive association between AMH and testosterone levels was observed in all groups. Also, serum AMH levels are negatively associated with maternal age in the population studied. PMID: 28758808
  8. Data confirm that serum levels of AMH decline as function of age (not menstrual cycle) in women ages 20-50; here, results of ELISA assays of serum levels of AMH vary according to kit used. These studies were conducted at a fertility clinic in Poland; serum AMH is proposed as biomarker for ovarian reserve. PMID: 28792788
  9. AMH single nucleotide polymorphism is associated with Endometriosis-associated infertility. PMID: 28831646
  10. This study demonstrated the existence of an AMH-FOXL2 relationship in hGCs. AMH is capable of increasing both gene and protein expression of FOXL2. Because FOXL2 induces AMH transcription, these ovarian factors could be finely regulated by a positive feedback loop mechanism to preserve the ovarian follicle reserve. PMID: 28660501
  11. Data suggest that circulating AMH influences development of human brain; high levels of circulating AMH are unique to developing boys; here, boys age 5-6 years exhibit up-regulation of circulating AMH but are delayed in maturity index (judged by drawings) as compared to girls age 5-6 years. This study was conducted in New Zealand. PMID: 28593614
  12. The combination of GDF9 + BMP15 potently stimulates AMH expression in primary cumulus cells. PMID: 28874516
  13. serum level elevated in polycystic ovary syndrome PMID: 27899014
  14. Surface plasmon resonance analysis showed no significant association between FS288 and AMHC , suggesting that FS288 indirectly regulates AMH signaling. Activin A, a direct target of FS288, did not itself induce reporter activity in P19 cells, but did prevent the FS288-induced increase in AMH signaling. Hence, local concentrations of FS288 and Activin A may influence the response of some cell types to AMH. PMID: 28500669
  15. The current study showed no significant difference in AMH level between Sudanese women with preeclampsia and the controls. AMH level was not associated with age, parity, gestational and body mass index. PMID: 28646929
  16. In a 46,XY phenotypically female patient bearing testes in inguinal canal, and diagnosed with complete androgen insensitivity syndrome, the dysfunctioning of AR by mutation enhanced AMH expression which ultimately leads to the failure in maturation of Sertoli cells. PMID: 28299491
  17. These findings underscore the importance of screening young females with AMH for possible occult primary ovarian insufficiency. PMID: 29176793
  18. Women with unexplained infertility did not show evidence of decreased ovarian reserve as measured by AMH and antral follicle count. PMID: 29202959
  19. An undetectable AMH level in women aged <40 at the end of chemotherapy for early stage breast cancer gave a good prediction that ovarian function would not return. PMID: 29117576
  20. In granulosa cells from women with PCOS, the regulation of AMH and AMHR2 expression is altered in a way that promotes the overexpression of the AMH/AMHR2 system, and could contribute to the follicular arrest observed in these patients. PMID: 28938480
  21. genetic variants of AMH or AMHR2 were not found to be associated with a higher risk for polycystic ovaries syndrome. PMID: 27664518
  22. Strong positive age-independent relationship between AMH level and the rate of euploid blastocysts I in vitro fertilization/intracytoplasmic sperm injection was found. PMID: 28987789
  23. The ratio of inactive proAMH precursor to receptor-competent AMHN,C differs in women with polycystic ovary syndrome relative to unaffected controls indicating that AMH signaling mechanisms may be altered in women with PCOS. PMID: 29079276
  24. Study found 24 rare AMH variants in patients with PCOS and control subjects; 18 variants were specific to women with PCOS. Seventeen of 18 (94%) PCOS-specific variants had significantly reduced AMH signaling, whereas none of 6 variants observed in control subjects showed significant defects in signaling. PMID: 28505284
  25. In the obese polycystic ovary syndrome group, anti-mullerian hormone was associated with ghrelin levels independent of age, insulin, and total testosterone. There was no association between total ghrelin and anti-mullerian hormone levels in non-obese women with polycystic ovary syndrome, non-obese controls, or obese controls. PMID: 28004236
  26. Data suggest that young men and young women initially have similar levels of circulating levels of AMH, but women lose their AMH in parallel with the decrease in their ovarian reserve. Circulating AMH in men is negatively associated with all-cause mortality; men with higher levels of AMH may outlive men with lower levels of AMH. [EDITORIAL] PMID: 27819112
  27. In young women with polycystic ovary syndrome, low AMH levels predict a greater risk of metabolic syndrome. PMID: 27842995
  28. MIS/AMH inhibits ovarian cancer by deregulating the Wnt signal pathway via the beta-catenin interacting protein (ICAT). MIS/AMH upregulated ICAT in ovarian cancer cell line which caused decreased cell viability, cell cycle arrest and apoptosis. PMID: 28197641
  29. The AMH level peaked at or before ovulation in most women, trended down with natural pregnancies, and consistently increased or decreased in women with a viable pregnancy after therapy. Nonviable pregnancies showed erratic AMH patterns. PMID: 27565260
  30. Before 35 years of age, women with type 1 diabetes have lower AMH levels than women without diabetes. PMID: 27475411
  31. Report variations in antimullerian hormone levels during the menstrual cycle. PMID: 27351446
  32. Suggest that activation of AMH by proteolytic enzymes is largely stable throughout the ovarian cycle. However, there is a subtle but robust decrease in the level of proAMH relative to AMHN,C in the acute postovulatory period. PMID: 27362611
  33. Age is correlated with AMH, but it accounts for only a portion of the variation seen in reproductive age African American women. PMID: 27114331
  34. AMH was inversely correlated with age in both the fertile and infertile populations and there was no significant difference between the fertile and infertile populations in terms of AMH. PMID: 27499425
  35. Data suggest that, in adolescents (as with adults) with PCOS (polycystic ovary syndrome), serum levels of 17-hydroxyprogesterone are variable as a characteristic of the disorder; 17-hydroxyprogesterone responsiveness to r-hCG (recombinant human chorionic gonadotropin, a fertility agent) is not correlated to serum levels of AMH (anti-Mullerian hormone). PMID: 27166718
  36. Anti-Mullerian hormone (AMH) and inhibin-A (INH-A) levels were found to be significantly higher in the polycystic ovary syndrome (PCOS) group compared to the controls. PMID: 27125339
  37. a significant positive correlation between serum asymmetric dimethylarginine and AMH levels in primary dysmenorrhea, is reported. PMID: 27523455
  38. Data suggest that the serum AMH level (a biomarker of ovarian reserve) in adolescent girls newly diagnosed with Hashimoto's thyroiditis (HT) is up-regulated compared to age- and BMI-matched control subjects; serum AMH levels are negatively correlated with serum biomarkers of oxidative stress in HT. PMID: 27343736
  39. Significantly lower serum AMH concentration was found in the regularly menstruating CKD women on hemodialysis in comparison with the healthy controls. 2. Serum AMH decreased significantly after successful kidney transplantation. PMID: 27553045
  40. AMH levels in serum and seminal plasma in healthy men and men with sperm pathology PMID: 27110929
  41. Genotyping of the AMH c.146G>T and AMHR2 -482A>G polymorphisms does not provide additional useful information as a predictor of ovarian reserve or ovarian response and treatment outcomes. PMID: 27142041
  42. The aim of this study was to investigate the density and distribution of single nucleotide polymorphisms (SNPs) anti-Mullerian hormone (AMH) and AMHRII receptors in cryptorchid patients. PMID: 27162065
  43. No evidence of significant associations of Ile49Ser and -482A>G with reproductive outcomes and polycystic ovary syndrome. PMID: 27832628
  44. Different phenotypes for polycystic ovary syndrome were identified with each related to different levels of anti-mullerian hormone. PMID: 26718304
  45. Data from a longitudinal, observational study suggest that, among women with type 1 diabetes, AMH levels in serum decline in manner similar to that previously reported in women without diabetes; thus, it is possible that AMH may be used to risk-stratify women with type 1 diabetes at risk for diminished ovarian reserve and poor reproductive outcomes in a similar manner as used in healthy women. PMID: 26798983
  46. Ile(49)Ser genotype not associated with estradiol levels, ovarian parameters, menstrual cycle length, or pregnancy outcomes in healthy Singapore women PMID: 26633196
  47. High serum Anti-Mullerian Hormone levels are associated with Polycystic Ovary Syndrome. PMID: 26761944
  48. Women with systemic lupus erythematosus demonstrated similar AMH levels as healthy controls, suggesting preserved ovarian reserve in this population. PMID: 26223296
  49. These findings contribute in clarifying the relationship between hormones regulating the early phase of steroidogenesis confirming that AMH is playing a suppressive role on CYP19A1 expression stimulated by gonadotropin in hGCs. Furthermore, a similar inhibitory effect for AMH was observed on P450scc gene expression when activated by gonadotropin treatment. PMID: 26631403
  50. The AMH and AMHRII gene polymorphisms were not found a significant difference in non-IR-PCOS and normal groups. To IR-PCOS women, genotypes of AMH were closely related to the serum levels of LH (P = 0.000), testosterone (P = 0.000) and HOMA-IR (P = 0.038), while in the non-IR-PCOS and normal groups, no relationship was found PMID: 26732661

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Involvement in disease Persistent Muellerian duct syndrome 1 (PMDS1)
Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

HGNC: 464

OMIM: 261550

KEGG: hsa:268

STRING: 9606.ENSP00000221496

UniGene: Hs.112432

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