Alternative Names
ACC 4 ELISA Kit; ACC 5 ELISA Kit; ACC4 ELISA Kit; ACC5 ELISA Kit; aleurain ELISA Kit; CATH_HUMAN ELISA Kit; cathepsin B3 ELISA Kit; cathepsin BA ELISA Kit; Cathepsin H heavy chain ELISA Kit; Cathepsin H light chain ELISA Kit; Cathepsin H mini chain ELISA Kit; CPSB ELISA Kit; CTSH ELISA Kit; minichain ELISA Kit; N benzoylarginine beta naphthylamide hydrolase ELISA Kit; N-benzoylarginine-beta-naphthylamide hydrolase ELISA Kit; pro cathepsin H ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.312 ng/mL-20 ng/mL
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<10% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
89-106 |
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| 1:2 |
Average % |
89 |
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| Range % |
82-97 |
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| 1:4 |
Average % |
94 |
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| Range % |
85-105 |
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| 1:8 |
Average % |
95 |
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| Range % |
85-101 |
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Recovery
| The recovery of human CTSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-100 |
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| EDTA plasma (n=4) |
95 |
87-105 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.142 |
2.099 |
2.121 |
1.934 |
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| 10 |
1.765 |
1.798 |
1.782 |
1.595 |
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| 5 |
1.264 |
1.295 |
1.280 |
1.093 |
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| 2.5 |
0.789 |
0.811 |
0.800 |
0.613 |
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| 1.25 |
0.604 |
0.621 |
0.613 |
0.426 |
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| 0.625 |
0.422 |
0.436 |
0.429 |
0.242 |
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| 0.312 |
0.302 |
0.299 |
0.301 |
0.114 |
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| 0 |
0.186 |
0.187 |
0.187 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
