Human flagellin ELISA Kit

Code CSB-E09671h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
flagellin
Abbreviation
flagellin
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Microbiology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human flagellin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %83-95
1:2Average %95
Range %91-99
1:4Average %95
Range %88-102
1:8Average %98
Range %91-104
Recovery
The recovery of human flagellin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8580-93
EDTA plasma (n=4)9592-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.955 2.960 2.958 2.804
252.577 2.505 2.541 2.387
12.51.686 1.799 1.743 1.589
6.250.849 0.888 0.869 0.715
3.120.431 0.411 0.421 0.267
1.560.223 0.242 0.233 0.079
0.780.201 0.205 0.203 0.049
00.155 0.152 0.154  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human flagellin ELISA Kit was designed for the quantitative measurement of Human flagellin protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .

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