Human flagellin ELISA Kit

Code CSB-E09671h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name flagellin
Abbreviation flagellin
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Microbiology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human flagellin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %89
Range %83-95
1:2Average %95
Range %91-99
1:4Average %95
Range %88-102
1:8Average %98
Range %91-104
The recovery of human flagellin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8580-93
EDTA plasma (n=4)9592-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
502.955 2.960 2.958 2.804
252.577 2.505 2.541 2.387
12.51.686 1.799 1.743 1.589
6.250.849 0.888 0.869 0.715
3.120.431 0.411 0.421 0.267
1.560.223 0.242 0.233 0.079
0.780.201 0.205 0.203 0.049
00.155 0.152 0.154  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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