Human fructosamin (FRA) ELISA kit

Instructions
Code CSB-EQ027728HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name fructosamin (FRA)
Abbreviation FRA
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.18 μmol/mL-12 μmol/mL
Sensitivity 0.04 μmol/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Recovery
The recovery of human FRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 88-96
EDTA plasma (n=4) 98 95-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μmol/ml OD1 OD2 Average Corrected
12 2.452 2.522 2.487 2.313
6 2.063 2.031 2.047 1.873
3 1.444 1.512 1.478 1.304
1.5 0.874 0.899 0.887 0.713
0.75 0.512 0.545 0.529 0.355
0.375 0.351 0.365 0.358 0.184
0.18 0.275 0.255 0.265 0.091
0 0.167 0.180 0.174  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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