Rat fructosamin (FRA) ELISA kit

Code CSB-E13451r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
fructosamin (FRA)
Abbreviation
FRA
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 nmol/mL-2000 nmol/mL
Sensitivity
7.81 nmol/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat FRA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %103
Range %99-108
1:2Average %98
Range %94-102
1:4Average %93
Range %89-97
1:8Average %90
Range %86-98
Recovery
The recovery of rat FRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)8984-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nmol/mlOD1OD2AverageCorrected
20002.362 2.458 2.410 2.229
10001.943 1.831 1.887 1.706
5001.206 1.241 1.224 1.043
2500.754 0.767 0.761 0.580
1250.395 0.413 0.404 0.223
62.50.318 0.335 0.327 0.146
31.250.221 0.234 0.228 0.047
00.179 0.182 0.181
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat FRA ELISA Kit was designed for the quantitative measurement of Rat FRA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 nmol/mL-2000 nmol/mL and the sensitivity is 7.81 nmol/mL.

Customer Reviews and Q&A

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 Q&A
Q:

Good day. May I ask a question about the kit, CSB-E13451r?
What is the standard? Fructosamines are compounds that result from glycation reactions between a sugar (such as fructose or glucose) and a primary amine.
How does this kit measure every type of fructosamine since many different proteins can be modified in this way?
Thank you for your help with my question.

A:
Thanks for your inquiry.
CSB-E13451r
The standard in the kit is modified NS0-derived recombinant protein.
As you mentioned: Fructosamines are compounds that result from glycation reactions between a sugar (such as fructose or glucose) and a primary amine. We have screened out specific raw materials of antibodies( mouse monoclonal antibody and goat polyclonal antibody). In theory, if the type of fructosamine has the antibody's specific recognition sites, then it can be detected out.
Pls let me know if you have any further questions. Thank you.
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