Human heme oxygenase 2,HO-2 ELISA Kit

Instructions
Code CSB-E08408h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name heme oxygenase (decycling) 2
Alternative Names HO-2, heme oxygenase (decyclizing) 2
Abbreviation HMOX2
Uniprot No. P30519
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HO-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 84-95
1:2 Average % 92
Range % 87-101
1:4 Average % 98
Range % 94-104
1:8 Average % 98
Range % 93-105
Recovery
The recovery of human HO-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-102
EDTA plasma (n=4) 91 84-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.447 2.292 2.370 2.222
10 1.959 1.820 1.890 1.742
5 1.161 1.251 1.206 1.058
2.5 0.706 0.763 0.735 0.587
1.25 0.406 0.416 0.411 0.263
0.625 0.290 0.298 0.294 0.146
0.312 0.221 0.223 0.222 0.074
0 0.144 0.152 0.148  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
Subcellular Location Microsome, Endoplasmic reticulum
Protein Families Heme oxygenase family
Database Links

HGNC: 5014

OMIM: 141251

KEGG: hsa:3163

STRING: 9606.ENSP00000219700

UniGene: Hs.284279

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