HMOX2 Antibody

Datasheet
Code CSB-PA010584HA01HU
Size US$299
Uniprot No. P30519
Image
  • Immunofluorescence staining of Hela cells with CSB-PA010584HA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunogen Recombinant Human Heme oxygenase 2 protein (2-293AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IF; Recommended dilution: IF:1:50-1:200
Relevance Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Heme oxygenase 2 (HO-2) (EC 1.14.14.18), HMOX2, HO2
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Immunofluorescence (IF) Protocol
Research Area Cell Biology
Target Names HMOX2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
Subcellular Location Microsome, Endoplasmic reticulum
Protein Families Heme oxygenase family
Database Links

HGNC: 5014

OMIM: 141251

KEGG: hsa:3163

STRING: 9606.ENSP00000219700

UniGene: Hs.284279

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