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Human hepatitis D virus(HDV) antigen ELISA Kit

Instructions
Code CSB-EQ027247HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name hepatitis D virus(HDV) antigen
Abbreviation HDV Ag
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range Request Information
Sensitivity Request Information
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Microbiology
Assay Principle qualitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Typical Data
             
             
Test parameter specification test result  
Positive control >0.6 0.789  
Negative control <0.1 0.054  
Positive rate 10，Positive 100%  
Negative rate 10，Negative 100%  
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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■ Q&A
Q:

what type of antigen was used to generate the antibodies of this kit? Was it a purified native protein/peptide or a recombinant/synthetic one

A:
Thanks for your mail!
The kit csb-eq027247hu sandwich enzyme immunoassay technique to detect HDV antigen.
The kit uses 2 strains of antibody, but the specific mmunogen information is our key development information and cannot be provided.
So sorry and appreciated to your kind understanding.
This kit is only used to detect serum and plasma sample.
Q:

A customer has the following question about item CSB-EQ027247HU:
Has it been validated by using recombinant HDV that is expressed by transfected human cells or has naturel HDV been used for validation?
If this assay works in their hands, she needs larger amounts for her study. Therefore she requires minimum inter (and of course intra) assay variations. Would this be possible to be guaranteed by the manufacturer?
Would you have this information available to share?

A:
Thanks for your inquiry!
CSB-EQ027247HU
This kit is for qualitative determination of antigen. It is suitable to test serum and plasma samples. Sorry we didn't test recombinant HDV before. We can not ensure 100% if this sample type could be tested out.
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Pls let me know if you have any further questions. Thank you.
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