Product Details

Target Name

macrophage stimulating 1 (hepatocyte growth factor-like)

Alternative Names

D3F15S2 ELISA Kit; DNF15S2 ELISA Kit; Hepatocyte growth factor like protein alpha chain ELISA Kit; Hepatocyte growth factor like protein ELISA Kit; Hepatocyte growth factor like protein beta chain ELISA Kit; Hepatocyte growth factor like protein homolog ELISA Kit; Hepatocyte growth factor-like protein beta chain ELISA Kit; HGFL ELISA Kit; HGFL_HUMAN ELISA Kit; Macrophage stimulating 1 (hepatocyte growth factor like) ELISA Kit; Macrophage stimulatory protein ELISA Kit; Macrophage-stimulating protein ELISA Kit; MSP ELISA Kit; MST1 ELISA Kit; NF15S2 ELISA Kit; OTTHUMP00000208927 ELISA Kit

Abbreviation

MST1

Uniprot No.

P26927

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	1.255	1.265	1.297	1.287	1.286	1.317
SD	0.038	0.043	0.035	0.044	0.044	0.053
CV(%)	4.822	5.409	4.294	5.439	5.446	6.401

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MSP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:20

Average %

101

Range %

94-108

1:40

Average %

105

Range %

99-109

1:80

Average %

Range %

82-96

1:160

Average %

Range %

87-99

Recovery

The recovery of human MSP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	104	97-113
EDTA plasma (n=4)	100	95-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



ng/ml	OD1	OD2	Average	Corrected
10	2.827	2.787	2.807	2.704
5	2.314	2.254	2.284	2.181
2.5	1.511	1.566	1.539	1.436
1.25	0.745	0.773	0.759	0.656
0.625	0.412	0.402	0.407	0.304
0.312	0.250	0.242	0.246	0.143
0.156	0.167	0.171	0.169	0.066
0	0.101	0.105	0.103

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human MST1 ELISA Kit was designed for the quantitative measurement of Human MST1 protein in serum, plasma, tissue homogenates_x005f_x000D_. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Background

Gene References into Functions

Mst1 has an important role in inhibiting the growth of NSCLC in vitro and in vivo; its antiproliferative effect is associated with induction of apoptosis. PMID: 23928732
These data suggest that MSP is an effective inhibitor of inflammation and lipid accumulation in the stressed liver, thereby indicating that MSP has a key regulatory role in non-alcoholic steatohepatitis. PMID: 27609031
Identify MST1/MSP as a mitogen for tracheal basal cells. PMID: 25551685
MSP appears to promote the migration of fibroblasts, enhances collagen synthesis and remodeling, and effectively improves wound healing. PMID: 25315688
Elevated serum levels of MST1 were found in subjects with excessive alcohol use. PMID: 25704570
These results support the hypothesis that the alpha-chain of MSPalphabeta mediates RON dimerization. PMID: 25193665
functional consequences of the macrophage stimulating protein 689C inflammatory bowel disease risk allele PMID: 24409221
Results suggest that the [AA] genotype of the common MST1 variant rs3197999 enhances genetic risk of sporadic extrahepatic cholangiocarcinoma irrespective of primary sclerosing cholangitis status. PMID: 23422030
MSP may be one of the major determinants that affects the properties of tumor stroma and that produces a permissive microenvironment to promote cancer metastasis PMID: 23011677
The present results refine the known genetic architecture in primary sclerosing cholangitis by confirming MST1 locus association. PMID: 22554193
the missense SNP impairs MSP function by reducing its affinity to RON and perhaps through a secondary effect on in vivo concentration arising from reduced thermodynamic stability, resulting in down-regulation of the MSP/RON signaling pathway. PMID: 22087277
HAT cleaves proMSP at the physiological activation site PMID: 22245154
Studies indicate that the cylindromatosis/turban tumor syndrome gene (CYLD) ranked highest, followed by acylaminoacyl-peptidase (APEH), dystroglycan (DAG1), macrophage-stimulating protein (MST1) and ubiquitin-specific peptidase 4 (USP4). PMID: 21931648
These findings suggest that the MSP/RON signaling pathway may be regulated by hepsin in tissue homeostasis and in disease pathologies, such as in cancer and immune disorders. PMID: 21875933
considerable number of Merkel cell carcinoma cases expressed both RON and MSP, while Merkel cells do not express these molecules PMID: 21723047
MSP-induced RSK2 activation is a critical determinant linking RON signaling to cellular EMT program. PMID: 21619683
the oncogenic effect of BRAF(V600E) is associated with the inhibition of MST1 tumor suppressor pathways, and that the activity of RASSF1A-MST1-FoxO3 pathways determines the phenotypes of BRAF(V600E) tumors. PMID: 21249150
results suggest that MSP induces uPAR expression via MAPK, AP-1 and NF-kappaB signaling pathways PMID: 21081472
Observational study and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20014019
These results suggest that activation of RON by macrophage-stimulating protein inhibits LPS-induced macrophage Cox-2 expression. PMID: 12177064
Data show that macrophage stimulating protein (MSP) and its receptor (Ron) induce phosphorylation of both Ron and alpha6beta4 integrin, and result in activation of alpha3beta1 integrin. PMID: 12919677
macrophage-stimulating protein-responsive cell growth in culture is suppressed by the ron-sema domain PMID: 14597639
Macrophage stimulating protein and its receptor RON are involved in the pathophysiology of endometriosis. PMID: 15764806
repression of MSP gene expression by mutant p53 may contribute to oncogenesis in a cell type-specific manner PMID: 16170349
overexpression of MSP, MT-SP1, and MST1R was a strong independent indicator of both metastasis and death in human breast cancer PMID: 17456594
Cell migration and production of inflammatory cytokines by the brain are enhanced by MSP stimulation in primary microglia. PMID: 18480548
The five gene transcripts (aldolase B, elafin, MST-1, simNIPhom and SLC6A14) were changed in patients with ulcerative colitis, and were related to the disease activity. PMID: 18700007
Gene-centric association mapping of chromosome 3p implicates MST1 in IBD pathogenesis. PMID: 19079170
Results suggest that hepatocyte growth factor activator is a major serum activator of pro-macrophage-stimulating protein. PMID: 19456860
The effect of BSN-MST1 locus on Crohn's disease predisposition was replicated, but no influence on ulcerative colitis or multiple sclerosis predisposition could be detected PMID: 19657358

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Involvement in disease

MST1 variant Cys-689 may be associated with inflammatory bowel disease (IBD), a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is unsure whether Cys-689 itself or a variation in linkage disequilibrium with Cys-689 is responsible for the association with IBD.

Subcellular Location

Secreted.

Protein Families

Peptidase S1 family, Plasminogen subfamily

Database Links

HGNC: 7380

OMIM: 142408

KEGG: hsa:4485

STRING: 9606.ENSP00000414287

UniGene: Hs.349110

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Code	CSB-E09062h
Size	96T,5×96T,10×96T
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Human macrophage stimulating protein,MSP ELISA Kit

Product Details

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Target Background