Human neuropeptide Y (NPY) ELISA kit

Code CSB-E08168h
Size 96T,5×96T,10×96T
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Product Details

Target Name
neuropeptide Y
Alternative Names
C-flanking peptide of NPY ELISA Kit; CPON ELISA Kit; Neuropeptide tyrosine ELISA Kit; Neuropeptide Y precursor ELISA Kit; NPY ELISA Kit; NPY_HUMAN ELISA Kit; Pro neuropeptide Y ELISA Kit; PYY 4 ELISA Kit; PYY4 ELISA Kit; Y Neuropeptide ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range
0.78 pg/mL-50 pg/mL
0.195 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 95  
Range % 82-101  
1:10 Average % 94  
Range % 86-103  
1:20 Average % 87  
Range % 82-94  
1:40 Average % 95  
Range % 88-100  
The recovery of human NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-100  
EDTA plasma (n=4) 90 85-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
50 2.653 2.561 2.607 2.458  
25 2.175 2.284 2.230 2.081  
12.5 1.596 1.574 1.585 1.436  
6.25 0.895 0.863 0.879 0.730  
3.13 0.517 0.542 0.530 0.381  
1.57 0.341 0.326 0.334 0.185  
0.78 0.278 0.266 0.272 0.123  
0 0.147 0.150 0.149    
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human NPY antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled NPY antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human NPY ELISA Kit was designed for the quantitative measurement of Human NPY protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.78 pg/mL-50 pg/mL and the sensitivity is 0.195 pg/mL .

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Target Background

(From Uniprot)
NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Gene References into Functions
  1. Neuropeptide Y (NPY) is a pleiotropic gene implicated in stress resilience and is associated with higher levels of conscientiousness. Along with environmental factors such as stressful life events, this gene may be a factor in the neurobiology of human personality. PMID: 29494882
  2. We found the suicide-associated gene coexpression network. The reconstructed network consisted of 104 genes. Topological analysis showed that in total, CCK, INPP1, DDC, and NPY genes are the most fundamental hubs in the network. PMID: 29381655
  3. Data indicated that NPY plays a protective role in ER stress-induced neuronal cell death through activation of the PI3K-XBP1 pathway. PMID: 29650257
  4. This study provided proof that SNPs within NPY is significantly associated with the susceptibility to and the prognosis of cervical vertigo. PMID: 29197114
  5. Data suggest that up-regulation of NPY inhibits proliferation of adipose-derived stem cells while promoting adipogenesis and up-regulating expression of white adipocyte biomarkers PPARG, CEBPA, CIDEC, and RIP140. (PPARG = peroxisome proliferator activated receptor gamma; CEBPA = CCAAT/enhancer-binding protein alpha; CIDEC = cell death-inducing DFFA-like effector C; RIP140 = nuclear receptor interacting protein 1) PMID: 28954935
  6. results are the first to demonstrate that functional NPY variation alters chronic stress-related vagal control, suggesting a potential parasympathetic role for NPY gene in stress regulation PMID: 27527739
  7. Medium chain triglyceride nutritional supplementation increased the amount of activated ghrelin and NPY in anorexia nervosa patients. PMID: 28361739
  8. Analysis indicates that low levels of NPY in psychogenic non-epileptic seizure patients may confer greater vulnerability to exhibit seizure-like symptoms and lower quality of life. PMID: 28927333
  9. The rs16147 genotype affected the reduction in insulin resistance and insulin levels in response to two different hypocaloric diets in obese subjects, with a lack of response in subjects with the major allele. PMID: 28787737
  10. We found that the NPY gene rs16147 SNP was related to the change in insulin levels, homeostasis model assessment-insulin resistance, CRP, IL-6 and waist circumference at 3 months after a hypocaloric low-fat diet. PMID: 27599771
  11. genetic variation in NPY and NPY2R is at low frequency and thus does not make a major contribution to the obese phenotype in the general population PMID: 28857123
  12. Genetic risk score (GRSNPY) analysis found twelve significant (P<0.05) serum NPY concentration related SNPs among alpha7 nicotinic acetylcholine receptor gene CHRNA7, insulin receptor gene INSR, leptin receptor gene LEPR, glucocorticoid receptor (GR) gene NR3C1, and NPY gene. However, after permutation test of gene score the predictive value of GRSNPY remained non-significant (P=0.078). CONCLUSIONS: Serum NPY level ... PMID: 27837667
  13. In obese males, the rs164147 polymorphism of the NPY gene is associated with leptin, insulin level, HOMA-IR, and an increased risk of MetS and its related phenotypes, such as central obesity and hyperglycemia. PMID: 27788523
  14. Both structural (+1128T/C) and promoter polymorphisms (-399 T/C) of Neuropeptide Y are strongly associated with type-II diabetes susceptibility in Gujarat population which at least in part, may result in higher levels of Neuropeptide Y thereby suggesting its crucial role in type-II diabetes susceptibility. PMID: 27749914
  15. Hypermethylation of WIF1 (WNT inhibitory factor 1) and NPY (neuropeptide Y) genes was significantly higher in tumor tissue compared to normal tissue, independently of tumor stage. PMID: 27251038
  16. Our results provided clinical evidences for NPY participating in the bone healing process in a nonhypothalamic manner, most probably by directly promoting osteogenesis of mesenchymal stem cells. PMID: 27720230
  17. In conclusion, the obtained results demonstrate the probable role of NPY SNPs in susceptibility to multiple sclerosis within the Iranian population. PMID: 27559040
  18. Neuropeptide Y is the best dipeptidyl peptidase 4 (DPP4)-substrate in blood, being truncated by soluble and membrane DPP4, respectively. The decline of soluble DPP4 in acute depression could be reversed upon anti-depressive treatment. PMID: 26988064
  19. The CT genotype of rs5574 and the GT genotype of rs17149106 are significantly associated with prevalent asthma. PMID: 27469060
  20. study implicates NPY as a potential target in antihypertensive therapies for preeclampsia patients PMID: 26431933
  21. NPY is efficiently cleaved by FAP indicating a potential function for FAP in neuropeptide regulation within liver and cancer biology. PMID: 26621486
  22. NPY plays important roles in the pathogenesis and pathophysiology of pituitary adenomas, as shown in this clinical study. PMID: 26683132
  23. NPY levels were higher in both obese and non-obese PCOS patients compared to healthy controls. PMID: 26291814
  24. minor autoantigen in children with newly diagnosed type 1 diabetes PMID: 25258030
  25. Authors observed that NPY levels were decreased in two Machado-Joseph disease patients' cerebella. PMID: 26220979
  26. Suggest IL-4 as marker of allergic airway inflammation in asthma, and roles for adiponectin and neuropeptide Y in neurohormonal signaling. PMID: 26111352
  27. This study suggests that NPY rs16147 T and rs16139 C minor alleles are associated with increased risk, whereas the minor allele T of the rs5574 is associated with a reduced risk of obesity. PMID: 26240981
  28. NPY expression is increased during atherogenesis and in particular in unstable plaques PMID: 24858338
  29. Immunohistochemical distribution of this peptide in the epidermal skin (from abdomen, breast and face) of healthy women was analysed. PMID: 26002416
  30. The C-terminal alpha-helix of Neuropeptide Y, which is formed in a membrane environment in the absence of the receptor, is unwound starting at T(32) to provide optimal contacts in a binding pocket within the transmembrane bundle of the NPY-Y2 receptor. PMID: 25924821
  31. Data suggest that nutritional status and up-regulation of NPY in cerebrospinal fluid are related to disease progression in adults with chronic kidney disease (CKD); up-regulation of serum NPY levels may predict risk of cardiovascular events in CKD. PMID: 25920420
  32. in AN patients, the NPY system is not up-regulated by chronic undernutrition suggesting that this may play a role in the inability of anorectic women to adapt food intake to their energy demand. PMID: 25798605
  33. The mean neuropeptide-Y level was 62.29 +/- 13.89 pg/mL in the breath-holding spells group and 58.24 +/- 12.30 pg/mL in the control group. PMID: 26021463
  34. neuropeptide Y has a role in Ewing sarcoma bone invasion PMID: 25714031
  35. study is the first to observe a sexually dimorphic role of alleles at NPY in humans and support previous genome-wide findings of a role of NPY in severe PD. PMID: 25256105
  36. Data suggest that an SNP in NPY (rs16147) is associated with change in abdominal adiposity in subjects with central obesity in response to dietary interventions (low/high fat); T allele carriers on high-fat diet regained abdominal fat. PMID: 26156739
  37. The data are in line with the hypothesis that NPY signaling is altered in affective disorders and states of emotional dysregulation. PMID: 25453484
  38. The rs16147 polymorphism may be associated with presence of metabolic syndrome (MetS) among subjects with documented coronary artery disease (CAD). Carriage of NPY A allele in patients with CAD is associated with a higher prevalence of MetS. PMID: 24897239
  39. This study suggests that the rs16147 polymorphism in the NPY gene may not be a potential contributor to the risk of CAD in an Iranian population. PMID: 25427865
  40. relationship existed between serum NPY and depressive disorders, posttraumatic stress disorder, and resilience. PMID: 24709369
  41. NPY may be a possible contributor to metabolic syndrome and associated cancer for the Han ethnic group. PMID: 25081719
  42. Distribution of peptidergic populations in the human dentate gyrus (somatostatin [SOM-28, SOM-12] and neuropeptide Y [NPY]) during postnatal development. PMID: 24965867
  43. Polymorphisms within NPY are associated with vitiligo susceptibility. PMID: 25221996
  44. A putative mechanism based on NPY-induced inhibition of voltage-dependent Ca(2+) influx in pre- and post-synaptic neurons is proposed. PMID: 25026432
  45. Systemic NPY levels are elevated in patients with Ewing sarcoma, and these high levels are associated with unfavorable disease features. PMID: 25387699
  46. NPY and its Y receptor are possible mediators of both vasoconstriction and pulmonary vascular remodelling in pulmonary hypertension PMID: 24779394
  47. Study demonstrated an interaction of NPY genotype and childhood emotional maltreatment on amygdala and posterior cingulate cortex activation during processing of emotional faces, in addition to a faster behavioral response PMID: 23482625
  48. In a series of human fetuses, neuropeptide Y-containing nerve fibers was present and could be detected as early as in the pineal of four- to five-month-old fetuses. PMID: 24757681
  49. BIBP-3226 was used to antagonize Y(1)-receptors, NPY to test the efficacy of the antagonism. PMID: 24213859
  50. Neuropeptide Y has fungicidal, antimicrobial activity against Candida albicans. PMID: 9756788

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Subcellular Location
Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.
Protein Families
NPY family
Tissue Specificity
One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
Database Links

HGNC: 7955

OMIM: 162640

KEGG: hsa:4852

STRING: 9606.ENSP00000242152

UniGene: Hs.1832

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