Human oxytocin,OT ELISA Kit

Code CSB-E08994h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation OT
Target Name oxytocin,OT
Alias N/A
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 8 μIU/mL-400 μIU/mL
Sensitivity 10 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 85-96  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 98  
Range % 92-103  
1:8 Average % 93  
Range % 87-98  
Recovery
The recovery of human OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-96  
EDTA plasma (n=4) 96 90-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average Corrected  
400 2.470 2.498 2.484 2.440  
160 1.530 1.496 1.513 1.469  
60 0.649 0.652 0.651 0.607  
20 0.299 0.313 0.306 0.262  
8 0.147 0.144 0.146 0.102  
0 0.045 0.043 0.044    
References
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Q&A and Customer Reviews

Q&A
Q:

I hope you would be able to advice me if this item would
be suitable for me to purchase in order to conduct human salivary
ELISA assay on oxytocin. Sample size is 60 and running in
duplicates.

A:
Thanks for your inquiry.
CSB-E08994h is based on double antibody sandwich method. Sorry we are not sure if it is suitable to test human salivary or not. We suggest you do a pretest first. One 96T kit can test 45 samples if you run in duplicates.
Customer Reviews

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