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Human oxytocin,OT ELISA Kit

Citations (9) Protocol MSDS
Code CSB-E08994h
Size 96T,5×96T,10×96T
Price Request a Quote
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
oxytocin,OT
Alternative Names
N/A
Abbreviation
OT
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
8 μIU/mL-400 μIU/mL
Sensitivity
10 μIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 89  
Range % 85-96  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 98  
Range % 92-103  
1:8 Average % 93  
Range % 87-98  
Recovery
The recovery of human OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-96  
EDTA plasma (n=4) 96 90-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average Corrected  
400 2.470 2.498 2.484 2.440  
160 1.530 1.496 1.513 1.469  
60 0.649 0.652 0.651 0.607  
20 0.299 0.313 0.306 0.262  
8 0.147 0.144 0.146 0.102  
0 0.045 0.043 0.044 　  
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Human oxytocin (OT) ELISA Kit was designed for the quantitative measurement of Human oxytocin (OT) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 8 μIU/mL-400 μIU/mL and the sensitivity is 10 μIU/mL.

Citations

Related Products

OXT ELISA kits

Customer Reviews and Q&A

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■ Q&A
Q:

I hope you would be able to advice me if this item would
be suitable for me to purchase in order to conduct human salivary
ELISA assay on oxytocin. Sample size is 60 and running in
duplicates.

A:
Thanks for your inquiry.
CSB-E08994h is based on double antibody sandwich method. Sorry we are not sure if it is suitable to test human salivary or not. We suggest you do a pretest first. One 96T kit can test 45 samples if you run in duplicates.
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