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Chicken ovotransferrin (OT) ELISA kit

Citations (1) Protocol
Code CSB-EL027592CH
Size 96T,5×96T,10×96T
Price Request a Quote
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
ovotransferrin (OT)
Abbreviation
OT
Uniprot No.
P02789
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, egg white
Detection Range
0.156 μg/mL-20 μg/mL
Sensitivity
0.156 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml

OD1

OD2

Average

20

0.177

0.184

0.181

10

0.214

0.227

0.221

2.5

0.423

0.428

0.426

0.625

0.866

0.849

0.858

0.156

1.293

1.321

1.307

0

2.038

2.153

2.096

ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Chicken ovotransferrin (OT) ELISA Kit was designed for the quantitative measurement of Chicken ovotransferrin (OT) protein in serum, plasma, egg white. It is a Competitive ELISA kit, its detection range is 0.156 μg/mL-20 μg/mL and the sensitivity is 0.156 μg/mL.

Citations

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. There are two forms of hen transferrin, ovotransferrin, found in the ovoducts and, serum transferrin, secreted by the liver. Serum transferrin may also have a role in stimulating cell proliferation and is regulated by iron levels. Ovotransferrin has a bacteriostatic function and, is not controlled by iron levels.
Subcellular Location
Secreted.
Protein Families
Transferrin family
Tissue Specificity
Expressed in the magnum of the oviduct (at protein level).
Database Links

KEGG: gga:396241

UniGene: Gga.2551

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