Human prostacycline,PGI2 ELISA Kit

Code CSB-E09591h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
prostacycline,PGI2
Abbreviation
PGI2
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
50 pg/mL-2000 pg/mL
Sensitivity
50 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Biochemicals
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-101  
1:2 Average % 94  
Range % 86-100  
1:4 Average % 94  
Range % 91-96  
1:8 Average % 95  
Range % 85-105  
Recovery
The recovery of human PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 88-98  
EDTA plasma (n=4) 96 92-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
0 2.666 2.517 2.592    
50 1.284 1.235 1.260    
150 0.625 0.641 0.633    
460 0.349 0.365 0.357    
1000 0.221 0.229 0.225    
2000 0.128 0.132 0.130    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PGI2 ELISA Kit was designed for the quantitative measurement of Human PGI2 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 50 pg/mL-2000 pg/mL and the sensitivity is 50 pg/mL.

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