Human prostacycline,PGI2 ELISA Kit

Code CSB-E09591h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name prostacycline,PGI2
Abbreviation PGI2
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 50 pg/mL-2000 pg/mL
Sensitivity 50 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Biochemicals
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-101  
1:2 Average % 94  
Range % 86-100  
1:4 Average % 94  
Range % 91-96  
1:8 Average % 95  
Range % 85-105  
Recovery
The recovery of human PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 88-98  
EDTA plasma (n=4) 96 92-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
0 2.666 2.517 2.592    
50 1.284 1.235 1.260    
150 0.625 0.641 0.633    
460 0.349 0.365 0.357    
1000 0.221 0.229 0.225    
2000 0.128 0.132 0.130    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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