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Rat prostacycline,PGI2 ELISA Kit

Citations (6) Protocol
Code CSB-E13706r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
prostacycline,PGI2
Abbreviation
PGI2
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.25 ng/mL-50 ng/mL
Sensitivity
0.125 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Biochemicals
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %96
Range %92-104
1:2Average %94
Range %91-98
1:4Average %102
Range %98-106
1:8Average %88
Range %82-94
Recovery
The recovery of rat PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)10398-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
500.223 0.222 0.223
12.50.356 0.351 0.354
40.546 0.557 0.552
10.794 0.804 0.799
0.251.173 1.275 1.224
02.404 2.305 2.355
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat PGI2 ELISA Kit was designed for the quantitative measurement of Rat PGI2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.25 ng/mL-50 ng/mL and the sensitivity is 0.125 ng/mL.

Citations

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