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Human protein Red (IK) ELISA kit

Human protein Red (IK) ELISA kit

Protocol MSDS

Code	CSB-EL011570HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

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Target Background

Product Details

Target Name

protein Red

Alternative Names

chondrosarcoma associated protein 2 ELISA Kit; CSA2 ELISA Kit; Cytokine IK ELISA Kit; IK ELISA Kit; IK cytokine down regulator of HLA II ELISA Kit; IK factor ELISA Kit; IK Gene ELISA Kit; prer protein ELISA Kit; Protein Red ELISA Kit; Protein RER ELISA Kit; RD element ELISA Kit; RED protein ELISA Kit; RED_HUMAN ELISA Kit; RER ELISA Kit

Abbreviation

Uniprot No.

Q13123

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

25 pg/mL-1600 pg/mL

Sensitivity

6.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-94
1:2	Average %	101
	Range %	98-104
1:4	Average %	92
	Range %	85-96
1:8	Average %	98
	Range %	94-102

Recovery

The recovery of human IK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	93	86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.909	2.965	2.937	2.763
800	2.215	2.332	2.274	2.100
400	1.462	1.487	1.475	1.301
200	0.900	0.923	0.912	0.738
100	0.555	0.564	0.560	0.386
50	0.363	0.378	0.371	0.197
25	0.243	0.249	0.246	0.072
0	0.173	0.174	0.174

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human protein Red (IK) ELISA Kit was designed for the quantitative measurement of Human protein Red (IK) protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)

Involved in pre-mRNA splicing as a component of the spliceosome. Auxiliary spliceosomal protein that regulates selection of alternative splice sites in a small set of target pre-mRNA species. Required for normal mitotic cell cycle progression. Recruits MAD1L1 and MAD2L1 to kinetochores, and is required to trigger the spindle assembly checkpoint. Required for normal accumulation of SMU1.; (Microbial infection) Required, together with SMU1, for normal splicing of influenza A virus NS1 pre-mRNA, which is required for the production of the exportin NS2 and for the production of influenza A virus particles. Not required for the production of VSV virus particles.

Gene References into Functions

IK inhibits Aurora B activation through recruiting PP2A into IK and Aurora B complex. PMID: 26906715
Data suggest an indirect radiosensitivity of hIK channels to low-dose photon irradiation with an impact on cell differentiation. PMID: 25277267
Data suggest that two human spliceosomal factors, RED and SMU1, are recruited to control expression and alternative splicing of viral exportins via PB2 (polymerase PB2) of influenza A virus (H3N2) and are required for efficient viral multiplication. PMID: 24945353
IK is an essential protein for achieving correct mitotic progress through the regulation of mitotic kinases and phosphatases PMID: 24996188
RED is required for kinetochore localization of MAD1, mitotic progression, and activation of the spindle assembly checkpoint. PMID: 22351768
IK gene expression is decreased in follicular variant of papillary thyroid carcinoma. PMID: 21509594
At least three genes, orthologous to loci in this LD block, HBEGF, IK, and SRA1, result independently in a phenotype of myocardial contractile dysfunction when their expression is reduced with morpholino antisense reagents. PMID: 19064678

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Subcellular Location

Nucleus. Nucleus, nucleoplasm. Chromosome. Cytoplasm, cytoskeleton, spindle pole.

Protein Families

RED family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 5958

OMIM: 600549

KEGG: hsa:3550

STRING: 9606.ENSP00000396301

UniGene: Hs.421245

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Human protein Red (IK) ELISA kit

Product Details

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Target Background