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Human soluble CD23(sCD23) ELISA Kit

Protocol
Code CSB-E14264h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Alternative Names
Blast 2 ELISA Kit; BLAST-2 ELISA Kit; Blast2 ELISA Kit; C type lectin domain family 4 member J ELISA Kit; C-type lectin domain family 4 ELISA Kit; C-type lectin domain family 4 member J ELISA Kit; C-type lectin domain family 4; member J ELISA Kit; CD 23 ELISA Kit; CD 23A ELISA Kit; CD23 ELISA Kit; CD23 antigen ELISA Kit; CD23A ELISA Kit; CLEC 4J ELISA Kit; CLEC4J ELISA Kit; Fc epsilon receptor II ELISA Kit; Fc epsilon RII ELISA Kit; Fc fragment of IgE ELISA Kit; Fc fragment of IgE low affinity II receptor for ELISA Kit; Fc fragment of IgE receptor II ELISA Kit; Fc fragment of IgE; low affinity II; receptor for (CD23) ELISA Kit; Fc of IgE ELISA Kit; Fc of IgE; low affinity II; receptor for (CD23) ELISA Kit; Fc receptor IgE low affinity II alpha polypeptide ELISA Kit; Fc receptor; IgE; low affinity II; alpha polypeptide; isoform CRA_a ELISA Kit; Fc-epsilon-RII ELISA Kit; FCE 2 ELISA Kit; FCE2 ELISA Kit; FCER 2 ELISA Kit; Fcer2 ELISA Kit; FCER2_HUMAN ELISA Kit; FCER2A ELISA Kit; FceRII ELISA Kit; IgE binding factor ELISA Kit; IgE receptor lymphocyte ELISA Kit; IgE-binding factor ELISA Kit; IGEBF ELISA Kit; Immunoglobulin E binding factor ELISA Kit; Immunoglobulin E receptor ELISA Kit; Immunoglobulin E receptor; low affinity II ELISA Kit; Immunoglobulin E-binding factor ELISA Kit; Immunoglobulin epsilon chain ELISA Kit; LEUKOCYTE ANTIGEN CD23 ELISA Kit; Low Affinity IgE Receptor ELISA Kit; Low affinity immunoglobulin epsilon Fc receptor ELISA Kit; Low affinity immunoglobulin epsilon Fc receptor membrane bound form ELISA Kit; Low affinity immunoglobulin epsilon Fc receptor soluble form ELISA Kit; Ly-42 ELISA Kit; Ly42 ELISA Kit; Lymphocyte antigen CD23 ELISA Kit; Lymphocyte IgE receptor ELISA Kit; MGC93219 ELISA Kit
Abbreviation
FCER2
Uniprot No.
P06734
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human sCD23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 95    
Range % 90-100    
1:2 Average % 88    
Range % 84-92    
1:4 Average % 97    
Range % 92-101    
1:8 Average % 85    
Range % 80-90    
Recovery
The recovery of human sCD23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 104 100-110    
EDTA plasma (n=4) 96 91-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
5000 2.651 2.495 2.573 2.418    
2500 1.903 1.827 1.865 1.710    
1250 1.390 1.306 1.348 1.193    
625 0.812 0.853 0.833 0.678    
312 0.549 0.529 0.539 0.384    
156 0.338 0.341 0.340 0.185    
78 0.264 0.260 0.262 0.107    
0 0.152 0.158 0.155 　    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

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Target Background

Function
(From Uniprot)
Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
Gene References into Functions
  1. Report a variant of t(14;18) negative nodal diffuse follicular lymphoma with CD23 expression, 1p36/TNFRSF14 abnormalities, and STAT6 mutations. PMID: 26965583
  2. High CD23 expression is associated with B-cell Lymphoma. PMID: 28108506
  3. found a higher frequency of LIN1(-) CCR3(+) eosinophils, and decreased expression of CD23 and CD62L receptors in eosinophils of AD patients PMID: 27406841
  4. there is a high CD23a/CD23b ratio of chronic lymphocytic leukemia cells, which demonstrates that in a subset of CLL cases, low CD23 expression together with high CD20 and CD38 expressions may serve as a surrogate for trisomy 12 PMID: 26119874
  5. Binding of CTLA4Fcepsilon to FcepsilonRII/CD23 appeared stronger than that of IgE PMID: 26801967
  6. Human and murine P2X7 activation induces the rapid shedding of CD23 from B cells, with a potential role for ADAM10 in this process. PMID: 25155463
  7. Elevated serum sCD23 is associated with increased risk of non-Hodgkin lymphoma. PMID: 25567136
  8. Data suggest that the protective immune response involving CD23-IgE-mediated nitric oxide release in cutaneous leishmaniasis (LCL). PMID: 25802003
  9. Crystal structure of the CD23-Fc3-4 complex with different molecular-packing constraints. PMID: 24598915
  10. data indicate that the studied FCER2 variations are strongly associated with asthma susceptibility and might be risk factor among north Indian asthmatic children PMID: 24102092
  11. we have demonstrated that PU.1, GATA1, and GATA2 are involved in the expression of FcepsilonRI in a human mast cell line and primary human mast cells using siRNA with high transfection efficiency, and by ChIP assay. PMID: 24639354
  12. CD23b-R62W-expressing human B cells bind IgE with greater affinity than wild-type cells and display differences in kinetics of CD23-mediated ERK1/2 activation PMID: 24010859
  13. The interplay of free IgE with IgE-allergen immune complexes of different sizes and composition with CD23 binding represents a mechanism for the modulation of CD23-mediated immune responses. PMID: 23229638
  14. Data indicate that the interaction of IgE with CD23 on B-cells regulates IgE production. PMID: 23933509
  15. Results suggest that binding of Ca(2+) brings an extra degree of modulation to CD23 function. PMID: 23775083
  16. The results are consistent with the suppression of parasite and allergen-specific IgE levels by sCD23. PMID: 23689700
  17. Data indicate that upon sialylation, the affinities for Fcgamma receptors are reduced, whereas those for alternative cellular receptors, such as dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN)/CD23, are increased. PMID: 23697368
  18. Results support the complex model of CD23 induction by Epstein-Barr virus (EBV). PMID: 23583387
  19. Downregulation of CD23 attenuated AIMP1-induced TNF-alpha secretion and AIMP1 binding. PMID: 22767513
  20. All subtypes of juvenile arthritis have reduced circulating levels of sCD21 and sCD23. PMID: 21328056
  21. CD23 and FcepsilonRI interaction sites are at opposite ends of the Cepsilon3 domain of IgE, but that receptor binding is mutually inhibitory, mediated by an allosteric mechanism PMID: 22815482
  22. Structural comparisons with both free IgE-Fc and its FcepsilonRI complex reveal not only that the conformational changes in IgE-Fc required for CD23 binding are incompatible with FcepsilonRI binding, but also that the converse is true. PMID: 22802656
  23. Binding of sCD23-derived peptides to av integrins and their biological activities are tolerant of some substitution in the recognition motif. PMID: 22560905
  24. Findings suggest that patients with t(11;14)(+)/CD23(+) plasma cell myelomas (PCM) present with lower platelet counts and may have a shorter overall survival than those with t(11;14)(+)/CD23(-) PCM. PMID: 21724429
  25. Variation at GLCCI1 and FCER2 could lead personalized asthma treatment. PMID: 22304573
  26. Soluble CD23 promoted the release of cytokines from the THP-1 model cell line. In both model cell lines and primary tissue, cytokine release was more pronounced in immature monocyte cells than in mature cells. PMID: 22348662
  27. mIgE & mCD21 cooperate in sCD23-mediated positive regulation of IgE synthesis on committed B cells. Feedback regulation may occur when the secreted-IgE level is big enough to allow binding to mCD23. This prevents further sCD23 release. PMID: 22393152
  28. Transfectants expressing the single nucleotide polymorphism in FCER2 exhibited increased IL-4Ralpha expression after stimulation through CD23 compared with wild-type. PMID: 22059556
  29. Our results suggest that the FCER2 T2206C variant might be a useful pharmacogenetic predictor of steroid refractory patients. PMID: 21958076
  30. SPR analysis revealed a progressive increment in affinity of soluble fragments for IgE, upon increasing length of CD23 "stalk" domain, exCD23>sCD23>derCD23. PMID: 21889131
  31. The association of CD23 and CD30 antigen blood levels with the development of non-Hodgkin lymphoma in HIV patients is reported. PMID: 21860351
  32. Report the role of CD23 SNPs in IgE dependent signaling in B-lymphocytes. PMID: 21686206
  33. IgE may be considered as immune mediator during antiprotozoal activity of human macrophages through its ability to trigger CD23 signaling. PMID: 21526166
  34. show for the first time that CD9 and CD81 act as molecular partners of trimeric FcvarepsilonRI on human antigen-presenting cell PMID: 21241315
  35. CD23 and CD19 are important factors that associated with serum total IgE in the pathogenesis of allergic rhinitis. PMID: 20359104
  36. CD23-mediated immunoglobulin (Ig)E transcytosis in human airway epithelial cells may play a critical role in initiating and contributing to the perpetuation of airway allergic inflammation. PMID: 21307287
  37. A model of CD23-bound IgE-mediated amplification of immunity by B cells is demonstrated in schistosomiasis. PMID: 21160045
  38. Data suggest that the CD23-mediated signal transduction pathways in human B cells and human monocytes are different. PMID: 20805040
  39. Data indicate that the regions of CD23 responsible for interaction with ligands have been identified and help to explain the structure-function relationships within the CD23 protein. PMID: 20831712
  40. CD23 is a useful marker in distinguishing mediastinal diffuse large B-cell lymphoma and classical Hodgkin lymphoma in mediastinal biopsies. PMID: 19223373
  41. Interaction of functional FCER2 promoter polymorphism and phenotype-associated haplotypes PMID: 19845913
  42. cd23 negativity is rare in typical b-cell CLL; negativity in patients with CD19+/CD5+ is suggestive of mantle cell leukemia PMID: 11920534
  43. prognostic significance of soluble CD23 in advanced stages of B-chronic lymphocytic leukemia and its role as an indicator for aggressive or indolent courses of disease. PMID: 12002758
  44. a review on the domains and functions of the cd23 receptor PMID: 12022472
  45. upstream regions of the CD23a and CD23b isoform coding sequences show distinct sensitivities to agents which induce CD23 protein expression at the plasma membrane PMID: 12070780
  46. Investigation of several stimulators to promote CD23a expression on CLL vs. normal B-cells confirmed a different CD23 regulation in B-CLL. CD23a is always predominantly expressed (constant ratio of CD23a:CD23b). PMID: 12127553
  47. CD23 expression in chronic lymphocytic leukemia was 53.9 times higher than in mantle-cell llymphoma. PMID: 12127555
  48. At term, the percentage of CD21(+) and CD23(+) B cells was comparable to the adult PMID: 12149502
  49. promoter is a target for NF-AT transcription factors in B-CLL cells PMID: 12379312
  50. The data show that monocyte-derived DC express the P2X7 receptor whose activation opens a cation-selective channel, and which leads to rapid and near complete shedding of CD23. PMID: 12456589

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Subcellular Location
Cell membrane; Single-pass type II membrane protein. Cell membrane; Lipid-anchor. Secreted. Note=Also exists as a soluble excreted form, sCD23.
Database Links

HGNC: 3612

OMIM: 151445

KEGG: hsa:2208

STRING: 9606.ENSP00000264072

UniGene: Hs.465778

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