Human trypsin ELISA Kit

Code CSB-E09827h
Size 96T,5×96T,10×96T
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Product Details

Target Name
protease, serine, 1 (trypsin 1)
Alternative Names
Alpha-trypsin chain 2 ELISA Kit; Beta-trypsin ELISA Kit; Cationic trypsinogen ELISA Kit; Digestive zymogen ELISA Kit; Nonfunctional trypsin 1 ELISA Kit; Prss1 ELISA Kit; Serine protease 1 ELISA Kit; TCR V beta 4.1 ELISA Kit; TRP1 ELISA Kit; TRY1 ELISA Kit; TRY1_HUMAN ELISA Kit; TRY4 ELISA Kit; TRYP1 ELISA Kit; Trypsin I ELISA Kit; Trypsinogen 1 ELISA Kit; Trypsinogen A ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
62.5 ng/mL-4000 ng/mL
15.6 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 95  
Range % 91-102  
1:2000 Average % 93  
Range % 88-99  
1:4000 Average % 89  
Range % 82-97  
1:8000 Average % 96  
Range % 91-108  
The recovery of human trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-99  
EDTA plasma (n=4) 96 90-104  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
4000 2.901 2.884 2.893 2.759  
2000 2.491 2.481 2.486 2.352  
1000 1.736 1.756 1.746 1.612  
500 0.942 0.955 0.949 0.815  
250 0.507 0.511 0.509 0.375  
125 0.462 0.473 0.468 0.334  
62.5 0.258 0.252 0.255 0.121  
0 0.132 0.136 0.134    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

The human Trypsin ELISA kit is suitable for the quantitative determination of human Trypsin in different sample types, including serum and plasma. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

Trypsin (PRSS1) is a serine protease produced in the pancreas in the precursor form of inactive trypsinogen by enterokinase. Trypsin is renowned for its role in enteric digestion. In the small intestine, trypsin breaks down proteins, continuing the process of digestion that began in the stomach. It has potent proteolytic activity, and inappropriate activation of trypsin may result in severe pathological states in various human diseases. Extra-pancreatic expression of trypsin has been detected in several cancer types. Trypsin is involved in colorectal carcinogenesis and promotes proliferation, invasion, and metastasis.

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Target Background

(From Uniprot)
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
Gene References into Functions
  1. TF-FVIIa/trypsin-mediated PAR2 activation leads to enhanced MMP-2 expression in human breast cancer cells contributing to tumor progression. PMID: 29870887
  2. report here for the first time a family from India that is positive for R122H mutation in the PRSS1 gene PMID: 29476405
  3. Studied mutations in cationic trypsinogen (PRSS1) and serine protease inhibitor Kazal type 1 (SPINK1) and their association with alcoholic chronic pancreatitis (ACP) and idiopathic chronic pancreatitis. PMID: 29641165
  4. Data indicate association between polymorphism rs10273639 of the PRSS1-PRSS2 locus and pancreatitis development. PMID: 27846138
  5. SNP in the PRSS1-PRSS2-rs10273639 is associated with Chronic pancreatitis. PMID: 28913878
  6. Gene mutations were present in PRSS1 in 26 patients with acute recurrent and chronic pancreatitis, SPINK1 in 23, CTRC in 3, and CPA1 in 5. In the 31 patients with mutations in SPINK1, CTRC, or CPA1, 16 (51.6%) had homozygous or heterozygous mutations with other mutations. PMID: 27409067
  7. Results identified 3 new rare variants in the proximal promoter region of PRSS1 which along the known c.-30_-28delTCC do not seem to be a risk factors for chronic pancreatitis. PMID: 27432637
  8. Early-onset pancreatitis is associated strongly with PRSS1 or CTRC mutations and family history of pancreatitis. PMID: 28502372
  9. PRSS1 variant p.L104P exhibits a variety of phenotypic changes that can increase risk for chronic pancreatitis. Mutation-induced misfolding and associated ER stress are the dominant effects that support a direct pathogenic role in chronic pancreatitis. PMID: 26822915
  10. Mutations in CFTR, SPINK1 or PRSS1 are present in one third of pediatric acute recurrent (ARP) or chronic (CP) pancreatitis with no other cause. PMID: 26692446
  11. Three types of pathogenic PRSS1 variants was identified in 11 patients, including p.N29I (n=1), p.R122H (n=1), and p.G208A (n=9). PMID: 27578509
  12. Mutations were found at the promoter and exon 3 of the PRSS1 in patients with pancreatic cancer. That is, five patients had c.410 C > T mutation causing p.Thr 137 Met, and three patients had c. -338 T >G mutation at the promoter of the PRSS1. In patients with PRSS1 mutations, serum trypsin was significantly higher than that in normal controls PMID: 26546433
  13. These findings suggest that IL-1beta plays a key role in trypsin upregulation and has a pathological role in multiple organ failure in influenza virus infection. PMID: 27714503
  14. Human anionic trypsinogen is controlled by CTRC in a manner that individual natural mutations are unlikely to increase stability enough to promote intra-pancreatic activation. PMID: 27129265
  15. Association of PRSS1 polymorphism with idiopathic recurrent acute pancreatitis and idiopathic chronic pancreatitis. PMID: 26110235
  16. Letter/Case Report: high penetrance of the PRSS1 A16V mutation caused by SPINK1 N34S and CFTR TG11-5T co-mutations associated with pancreatitis. PMID: 26658045
  17. We did not find PRSS1 mutation in any patient. PMID: 25981744
  18. In Israel, pediatric as well as adult recurrent acute pancreatitis and chronic pancreatitis are often associated with PRSS1, serine protease inhibitor Kazal type 1, chymotrypsin C, or Cystic Fibrosis Transmembrane Conductance Regulator mutations. PMID: 25383785
  19. This study shows that PRSS1, SPINK1 and CFTR mutations do not play a role in alcoholic and idiopathic chronic pancreatitis patients. PMID: 25835118
  20. N29T mutation associated with pancreatitis PMID: 26376395
  21. Recombination events between members of the trypsinogen family can generate high-risk PRSS1 alleles. PMID: 25546417
  22. the observations demonstrate that the tyrosyl sulfate in human trypsins interacts with the P2' position of the substrate-like inhibitor and this modification increases P2' selectivity towards basic side chains. PMID: 25010489
  23. PRSS1 single nucleotide polymorphism is associated with alcoholic pancreatitis. PMID: 25253127
  24. PRSS1 variants are associated with less disease relapse in autoimmune pancreatitis. PMID: 24909264
  25. Two novel PRSS1 mutations were identified in Mexican patients with early onset idiopathic recurrent acute pancreatitis. PMID: 25206283
  26. The -409 T/T genotype of PRSS1 gene was revealed to be a marker for predicting neotal sepsis, along with serum trypsin levels. PMID: 24777884
  27. None of the selected 121 pancreatic cancer samples showed a pancreatitis-predisposing mutation in the PRSS1 gene. PMID: 25003218
  28. expression of WT or mutant human PRSS1 in murine acinar cells induces apoptosis and is sufficient to promote spontaneous pancreatitis. PMID: 24722290
  29. Using the optimal cutoffs, the sensitivity of the IRT/PAP strategy would not be inferior to that of IRT/DNA if identification of mild forms is not required. PMID: 24513262
  30. PRSS1: IVS 2 +56_60 del CCCAG is a noval mutant which may contribute to AIP pathogenesis. PMID: 24236450
  31. The p.G208A variant of PRSS1, previously only found in Asian patients, was found in 3 generations of a European family, one of whom had chronic pancreatitis. PMID: 24413785
  32. the p.N43S mutation in the SPINK1 gene, accelerated the onset of HP in the presence of the p.R122H mutation in the CT gene. PMID: 24134754
  33. Rare causative PRSS1 mutations account for 9.1% of the idiopathic chronic pancreatitis study group in 253 young French patients. PMID: 23951356
  34. The trypsinogen to trypsin conformational switch modulates cleavage susceptibility of nick sites by altering both the thermodynamics and kinetics of cleavage to protect human cationic trypsin from premature degradation. PMID: 24403079
  35. PRSS1 hereditary pancreatitis is characterized by progressive lipomatous atrophy of the pancreas. PMID: 24525505
  36. No association of PRSS1 with pancreatic cancer is found. PMID: 23751316
  37. D21A polymorphism in the PRSS1 gene seems to be a risk factor for hereditary chronic pancreatitis. PMID: 23864476
  38. PRSS1 gene was significantly associated with total pancreatitis disease, both totally and separately PMID: 23801884
  39. For pediatric ulcerative colitis patients who require surgery, the immunopositivity of Tryp-1 at diagnosis is lower when compared to that of patients with a more benign disease course. PMID: 23745029
  40. Autoimmune pancreatitis may be related to PRSS1 gene mutations. PMID: 23745036
  41. Trypsin-1 and trypsin-2 appear to have a function in the degradation of vitreous type II collagen. PMID: 23882137
  42. A kindred from Venezuela, South America with the PRSS1 R122H variant. PMID: 23474566
  43. Even in the absence of chymotrypsin C, trypsinogen activation peptide mutants autoactivated at markedly increased rates. PMID: 23601753
  44. Case Report: suggest involvement of PRSS1 mutations in familial solid pseudopapillary pancreatic tumors. PMID: 22722260
  45. Frequencies of CFTR variants p.R75Q, p.I148T, 5T-allele and p.E528E were comparable in patients and controls. We identified 103 CFTR variants, which represents a 2.7-fold risk increase (p<0.0001). PMID: 22427236
  46. Two associations with alcoholic pancreatitis at genome-wide significance were identified and replicated at PRSS1-PRSS2 and X-linked CLDN2 through a two-stage genome-wide study. PMID: 23143602
  47. Data show that among the 2 mutations in the PRSS1 gene, only the N29I mutation was found in 1 (1.7%) of 58 patients with AP, in the heterozygous state. PMID: 22699143
  48. hereditary pancreatitis is caused by CTRC-dependent dysregulation of cationic trypsinogen autoactivation, which results in elevated trypsin levels in the pancreas. PMID: 22539344
  49. All of the heterozygote patients with a combination of CFTR and SPINK1 or PRSS1 mutations had chronic pancreatitis. PMID: 22094894
  50. Mutations of PRSS1 gene may be an important factor of pancreatic cancer. PMID: 22088471

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Involvement in disease
Pancreatitis, hereditary (PCTT)
Subcellular Location
Secreted, extracellular space.
Protein Families
Peptidase S1 family
Database Links

HGNC: 9475

OMIM: 167800

KEGG: hsa:5644

STRING: 9606.ENSP00000308720

UniGene: Hs.382212

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