Code | CSB-E09827h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1000 | Average % | 95 | ||||
Range % | 91-102 | |||||
1:2000 | Average % | 93 | ||||
Range % | 88-99 | |||||
1:4000 | Average % | 89 | ||||
Range % | 82-97 | |||||
1:8000 | Average % | 96 | ||||
Range % | 91-108 |
The recovery of human trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 89-99 | ||||
EDTA plasma (n=4) | 96 | 90-104 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human Trypsin ELISA kit is suitable for the quantitative determination of human Trypsin in different sample types, including serum and plasma. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.
Trypsin (PRSS1) is a serine protease produced in the pancreas in the precursor form of inactive trypsinogen by enterokinase. Trypsin is renowned for its role in enteric digestion. In the small intestine, trypsin breaks down proteins, continuing the process of digestion that began in the stomach. It has potent proteolytic activity, and inappropriate activation of trypsin may result in severe pathological states in various human diseases. Extra-pancreatic expression of trypsin has been detected in several cancer types. Trypsin is involved in colorectal carcinogenesis and promotes proliferation, invasion, and metastasis.
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