Product Details

Target Name

maltase-glucoamylase (alpha-glucosidase)

Alternative Names

4-alpha-glucosidase ELISA Kit; Alpha-glucosidase ELISA Kit; Glucan 1 ELISA Kit; Glucoamylase ELISA Kit; Maltase-glucoamylase; intestinal ELISA Kit; MGA ELISA Kit; MGA_HUMAN ELISA Kit; MGAM ELISA Kit; MGAML ELISA Kit

Abbreviation

MGAM

Uniprot No.

O43451

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, urine

Detection Range

3.12 μU/mL-200 μU/mL

Sensitivity

0.78 μU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human α-Glucosidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	95
	Range %	85-103
1:40	Average %	90
	Range %	85-99
1:80	Average %	95
	Range %	91-102
1:100	Average %	89
	Range %	81-97

Recovery

The recovery of human α-Glucosidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-101
EDTA plasma (n=4)	93	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µU/ml	OD1	OD2	Average	Corrected
200	2.127	2.112	2.120	1.913
100	1.746	1.757	1.752	1.545
50	1.442	1.417	1.430	1.223
25	1.069	1.028	1.049	0.842
12.5	0.835	0.843	0.839	0.632
6.25	0.502	0.521	0.512	0.305
3.12	0.347	0.354	0.351	0.144
0	0.205	0.209	0.207

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human MGAM ELISA Kit was designed for the quantitative measurement of Human MGAM protein in serum, plasma, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 3.12 μU/mL-200 μU/mL and the sensitivity is 0.78 μU/mL.

Target Background

Function
(From Uniprot)

May serve as an alternate pathway for starch digestion when luminal alpha-amylase activity is reduced because of immaturity or malnutrition. May play a unique role in the digestion of malted dietary oligosaccharides used in food manufacturing.

Gene References into Functions

Mechanistic Pathway on Human alpha-Glucosidase Maltase-Glucoamylase Unveiled by QM/MM Calculations PMID: 29548257
MGAM, or nearby regulatory elements, may be involved in the etiology of oral clefts. PMID: 25776870
Starch internal structure modulates its susceptibility to MGAM. The internal branch amounts negatively affect the glucose release rate. PMID: 25037326
Findings suggest that C-terminal subunits of recombinant maltase-glucoamylase (MGAM) assists alpha-amylase in digesting starch molecules and potentially may compensate for developmental or pathological amylase deficiencies. PMID: 22563462
These results suggest that the N-terminal and C-terminal catalytic domains of maltase-glucoamylase differ in their substrate specificities and inhibitor tolerance despite their structural relationship PMID: 22036121
we report crystal structures of C-terminal maltase-glucoamylase alone at a resolution of 3.1 angstroms, and in complex with its inhibitor acarbose PMID: 22058037
analysis of substrate selectivity of human maltase-glucoamylase and sucrase-isomaltase N-terminal domains PMID: 20356844
genetic analysis of MGAM, exon boundaries, and chromosome mapping PMID: 12547908
Raw starch granule degradation with recombinanat human MGAM indicates that pancreatic alpha-amylase hydrolysis is not a requirement for native starch digestion in the human small intestine. PMID: 17485087
Intestinal maltase-glycoamylase: crystal structure of the N-terminal catalytic subunit and basis of inhibition and substrate specificity. PMID: 18036614
Acarbose has been found to improve insulin levels and thus glucose/insulin ratios more effectively in overweight patients compared with nonoverweight patients with PCOS. PMID: 18377903
This study reported the first diagnosed Finnish patient with a phenotype compatible with the late-onset form of Pompe disease. Molecular genetic analysis of the GAA gene revealed a novel missense mutation (Y575X),combined with (P545L) mutation. PMID: 19472353

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Subcellular Location

Apical cell membrane; Single-pass type II membrane protein. Note=Brush border.

Protein Families

Glycosyl hydrolase 31 family

Tissue Specificity

Expressed in small intestine, granulocyte, and kidney but not in salivary gland or pancreas.

Database Links

HGNC: 7043

OMIM: 154360

KEGG: hsa:8972

STRING: 9606.ENSP00000447378

UniGene: Hs.122785

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Code	CSB-E09459h
Size	96T,5×96T,10×96T
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Human α-Glucosidase ELISA Kit

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Target Background