Target Name
hemoglobin, alpha 2
Alternative Names
Alpha 1 globin ELISA Kit; Alpha globin ELISA Kit; Alpha one globin ELISA Kit; Alpha-globin ELISA Kit; HBA_HUMAN ELISA Kit; HBA1 ELISA Kit; HBA2 ELISA Kit; Hemoglobin alpha 1 ELISA Kit; Hemoglobin alpha 1 chain ELISA Kit; Hemoglobin alpha 1 globin chain ELISA Kit; Hemoglobin alpha 2 ELISA Kit; Hemoglobin alpha chain ELISA Kit; Hemoglobin subunit alpha ELISA Kit; MGC126895 ELISA Kit; MGC126897 ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, lysate for RBC
Detection Range
0.170 ng/mL-700 ng/mL
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | | |
Inter-assay Precision (Precision between assays): CV%<10% | | | | |
Three samples of known concentration were tested in twenty assays to assess. | | | |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HB-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| Sample | Serum(n=4) | | |
1:100 | Average % | 93 | | |
Range % | 84-103 | | |
1:200 | Average % | 91 | | |
Range % | 85-99 | | |
1:400 | Average % | 95 | | |
Range % | 85-103 | | |
1:800 | Average % | 91 | | |
Range % | 83-98 | | |
Recovery
The recovery of human HB-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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Sample Type | Average % Recovery | Range | | |
Serum (n=5) | 95 | 88-101 | | |
EDTA plasma (n=4) | 95 | 87-102 | | |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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ng/ml | OD1 | OD2 | Average | | | | 700 | 0.248 | 0.252 | 0.250 | | | | 175 | 0.441 | 0.435 | 0.438 | | | | 44 | 0.528 | 0.514 | 0.521 | | | | 11 | 0.712 | 0.723 | 0.718 | | | | 2.7 | 0.867 | 0.861 | 0.864 | | | | 0.7 | 1.119 | 1.107 | 1.113 | | | | 0.17 | 1.738 | 1.716 | 1.727 | | | | 0 | 1.935 | 1.922 | 1.929 | | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx