Monkey Complement 3(C3)ELISA Kit

Code CSB-E16528Mk
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Monkey Complement 3 (C3) ELISA Kit was designed for the quantitative measurement of Monkey Complement 3 (C3) protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 4.68 ng/mL-300 ng/mL and the sensitivity is 1.17 ng/mL.

Target Name Complement 3(C3)
Alternative Names C3Complement C3 [Cleaved into: Complement C3 beta chain; Complement C3 alpha chain; C3a anaphylatoxin; C3-beta-c ELISA kit; C3bc); Acylation stimulating protein ELISA kit; ASP ELISA kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA kit
Abbreviation C3
Uniprot No. Q2UVX4
Species Monkey
Sample Types serum, plasma, cell culture supernates
Detection Range 4.68 ng/mL-300 ng/mL
Sensitivity 1.17 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
300 2.276 2.296 2.286 2.144  
150 1.896 1.854 1.875 1.733  
75 1.520 1.539 1.530 1.388  
37.5 1.195 1.174 1.185 1.043  
18.75 0.864 0.883 0.874 0.732  
9.37 0.531 0.557 0.544 0.402  
4.68 0.345 0.365 0.355 0.213  
0 0.141 0.143 0.142    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Monkey

Review: The difference between the kit batches is small, and the test repeatability is better!

By Anonymous

Target Background

Function
(From Uniprot)
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.; Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils.; Acts as a chemoattractant for neutrophils in chronic inflammation.; adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2.
Gene References into Functions
  1. The structure of bovine C3 clearly demonstrates that the main chain around the thioester undergoes a helical transition upon activation, a rearrangement that is proposed to be the basis for the high level of reactivity of the thioester group. PMID: 16831446
Subcellular Location Secreted.
Database Links

KEGG: bta:280677

STRING: 9913.ENSBTAP00000022979

UniGene: Bt.19562

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