Mouse Apolipoprotein C-III(APOC3) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

apolipoprotein C-III

Alternative Names

Apoc3Apolipoprotein C-III ELISA kit; Apo-CIII ELISA kit; ApoC-III ELISA kit; Apolipoprotein C3 ELISA kit

Abbreviation

APOC3

Uniprot No.

P33622

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

18.75 ng/mL-1200 ng/mL

Sensitivity

9.375 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	94
	Range %	90-98
1:200	Average %	91
	Range %	88-95
1:400	Average %	83
	Range %	80-87
1:800	Average %	88
	Range %	84-92

Recovery

The recovery of mouse APOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	86	82-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
1200	0.087	0.089	0.088
600	0.188	0.191	0.190
300	0.382	0.378	0.380
150	0.653	0.645	0.649
75	1.131	1.146	1.139
37.5	1.516	1.527	1.522
18.75	1.819	1.921	1.870
0	2.369	2.265	2.317

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse APOC3 ELISA Kit was designed for the quantitative measurement of Mouse APOC3 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 18.75 ng/mL-1200 ng/mL and the sensitivity is 9.375 ng/mL.

Target Background

Function
(From Uniprot)

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.

Gene References into Functions

Both triglyceride-rich lipoproteins (TRLs) and ApoCIII contribute to the progression of atherosclerosis, and the modulation of TRLs and ApoCIII may represent a novel therapeutic approach against hypertriglyceridemia induced atherosclerosis. PMID: 30223835
C3(QK) variant is a gain-of-function mutation that can stimulate VLDL1 production, through enhanced DNL PMID: 28887372
intestinal apoC-III overexpression results in the secretion of smaller, less dense chylomicron particles along with reduced triacylglycerol secretion from the intestine PMID: 28159868
ApoC-III inhibits turnover of TG-rich lipoproteins primarily through a hepatic clearance mechanism mediated by the LDLR/LRP1 axis PMID: 27400128
These data strongly suggest that intestinal apoC-III is not a FoxO1 target and support the idea that apoC-III is not regulated coordinately with hepatic apoC-III, and establishes another key aspect of apoC-III that is unique in the intestine from the liver. PMID: 28739253
APOC3, whose dysregulation is liable for hypertriglyceridemia, is not a predisposing factor for linking overnutrition to NAFLD in obesity PMID: 28115523
Severe hypertriglyceridaemia resulting from ApoCIII overexpression promotes restenosis and atherosclerosis PMID: 26160324
Under conditions of islet insulin resistance, locally produced apoCIII is an important diabetogenic factor involved in impairment of beta-cell function. PMID: 25941406
decreased ApoAI synthesis might be accounted for the lower plasma HDL level in ApoCIII transgenic mice PMID: 25969427
ApoCIII hyperactivates beta cell CaV1 channels through SR-BI/beta1 integrin-dependent coactivation of PKA and Src. PMID: 23949443
Increased plasma APOC3 concentrations predispose mice to diet-induced nonalcoholic fatty liver and hepatic insulin resistance. PMID: 21793029
Glucose induces apoCIII transcription, which may represent a mechanism linking hyperglycemia, hypertriglyceridemia, and cardiovascular disease in type 2 diabetes. PMID: 21183731
PGC-1beta regulates plasma triglyceride metabolism through stimulating apolipoprotein C3 (APOC3) expression and elevating APOC3 levels in circulation PMID: 20889132
The apoC-III metabolism may contribute to dyslipidemia in CKD, and this requires further investigation. PMID: 19542564
Association between SstI polymorphism of the gene, glucose intolerance and cardiovascular risk in renal transplant recipients PMID: 11959336
ApoB lipoproteins that contain apoCIII increase THP-1 cell adhesion to ECs via PKCalpha and RhoA-mediated beta1-integrin activation. PMID: 16461842
oxidized fatty acids may act through an APOA5/APOClll mechanism that contributes to lowering of TG levels other than PPAR* induction PMID: 18243209
the apoCIII enhancer contributes to the maintenance of an active chromatin subdomain of the apoAI/CIII/AIV genes, but not apoAV PMID: 18678879
These results suggest that apoC-III may play a specific role in lipid storage and mobilization in adipocytes, non-lipoprotein-secreting cells, and indicate the functional role of RXRalpha during adipocyte differentiation. PMID: 19121312

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Subcellular Location

Secreted.

Protein Families

Apolipoprotein C3 family

Database Links

KEGG: mmu:11814

STRING: 10090.ENSMUSP00000034586

UniGene: Mm.390161

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Code	CSB-EL001933MO
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.