Mouse Apolipoprotein D(APOD) ELISA kit

Code CSB-EL001935MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein D
Alternative Names ApodApolipoprotein D ELISA kit; Apo-D ELISA kit; ApoD ELISA kit
Abbreviation APOD
Uniprot No. P51910
Species Mus musculus (Mouse)
Sample Types serum, plasma, urine
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 97  
Range % 92-106  
1:2000 Average % 90  
Range % 83-99  
1:4000 Average % 89  
Range % 86-99  
1:8000 Average % 92  
Range % 87-102  
The recovery of mouse APOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 87-103  
EDTA plasma (n=4) 94 91-99  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
200 1.670 1.702 1.686 1.572  
67 1.513 1.561 1.537 1.423  
22 1.097 1.142 1.120 1.006  
7.4 0.626 0.645 0.636 0.522  
2.5 0.359 0.368 0.364 0.250  
0.8 0.178 0.169 0.174 0.060  
0.27 0.124 0.127 0.126 0.012  
0 0.115 0.112 0.114    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function APOD occurs in the macromolecular complex with lecithin-transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.
Gene References into Functions
  1. Study shows that in myelin maturation ApoD ultimately controls the removal of the sialic-rich hydrophilic glycocalyx, by maintaining functional integrity of lysosomes. A detailed analysis of the mechanism reveals that the proper localization of Neu1 and plasma membrane Neu3, as well as of the membrane-bound Fyn kinase, depend on ApoD. PMID: 29222871
  2. apoD protein levels are variable across different brain regions. PMID: 26829325
  3. It regulates amyloid plaque pathology in a mouse model of Alzheimer's disease PMID: 25784209
  4. apoD may be part of an intrinsic cardioprotective system, possibly as a consequence of its antioxidation activity. PMID: 24082102
  5. Changes in hypothalamus of ApoD(-/-) mice may indicate potential role of ApoD in regulation of endocrine functions of somatostatin in a receptor-dependent manner. PMID: 22581439
  6. Abeta25-35 induces neuroprotective ApoD expression in hippocampal cells in response to stress-induced growth arrest. PMID: 22398376
  7. The findings underscore the importance of ApoD in the regulation of plasma insulin levels and triglyceride metabolism, suggesting that ApoD plays an important role in the pathogenesis of dyslipidemia. PMID: 21632073
  8. The data of this study supported that ApoD contributes to the endurance of astrocytes and decreases their reactivity level in vitro and in vivo PMID: 21688324
  9. Scanning cytometry revealed PACAP/ApoD induced low density lipoprotein receptors (LDLR) and ApoE receptor 2 (apoER2) in NF200-positive cells. PMID: 20920539
  10. mRNA and protein expression profiles reveal that ApoD is functionally connected in an age-dependent manner to specific molecular programs triggered by injury. PMID: 20607718
  11. Apolipoprotein D plays a significant role in lipid metabolism. PMID: 20124557
  12. This study concluded that glutamatergic pathways seem to be particularly affected in ApoD(-/-) mice. PMID: 19963028
  13. Expression pattern of the lipocalin apolipoprotein D during mouse embryogenesis. PMID: 11744388
  14. Apolipoprotein D-immunolabeled cells are observed in select grey matter nuclei, including the globus pallidus, thalamus, and substantia nigra, and in white matter tracts within the internal capsule and cerebellum of Neimann-Pick type C mouse brain. PMID: 12815234
  15. apolipoprotein D and the lipogenic protein Spot 14 are LXR alpha and LXRB responsive genes both in vitro and in vivo PMID: 14703507
  16. ApoD messenger RNA expression was localised to the spiral ligament and spiral limbus, particularly in the suprastrial and supralimbral regions. PMID: 15668042
  17. These observations, together with its transcriptional up-regulation in the brain upon oxidative insult, identify ApoD as an acute response protein with a protective and therefore beneficial function mediated by the control of peroxidated lipids. PMID: 18419796
  18. Taken together, our results describe receptor and region specific changes in SST and SSTR subtypes expression in ApoD(-/-) mice brain, which may be linked to specific neurological disorders. PMID: 19465111

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Subcellular Location Secreted
Protein Families Calycin superfamily, Lipocalin family
Tissue Specificity Highest levels of expression in brain, testis, virgin mammary gland and salivary gland. Moderate levels in skeletal muscle, lactating mammary gland and thymus. Low levels in lung and lymph node. No expression in kidney, pancreas, liver or spleen.
Database Links

KEGG: mmu:11815

STRING: 10090.ENSMUSP00000110885

UniGene: Mm.2082


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