Mouse Cytochrome P450 2E1(CYP2E1) ELISA kit

Code CSB-EL006425MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
cytochrome P450, family 2, subfamily E, polypeptide 1
Alternative Names
Cyp2e1 ELISA kit; Cyp2e ELISA kit; Cyp2e-1Cytochrome P450 2E1 ELISA kit; EC 1.14.14.1 ELISA kit; 4-nitrophenol 2-hydroxylase ELISA kit; EC 1.14.13.n7 ELISA kit; CYPIIE1 ELISA kit; Cytochrome P450-ALC ELISA kit; Cytochrome P450-J ELISA kit
Abbreviation
CYP2E1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
39 pg/mL-2500 pg/mL
Sensitivity
9.75 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 80-92
1:2 Average % 96
Range % 92-100
1:4 Average % 91
Range % 85-95
1:8 Average % 104
Range % 100-113
Recovery
The recovery of mouse CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-100
EDTA plasma (n=4) 92 88-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2500 2.482 2.658 2.570 2.384
1250 1.968 2.076 2.022 1.836
625 1.418 1.503 1.461 1.275
312 0.833 0.857 0.845 0.659
156 0.555 0.521 0.538 0.352
78 0.315 0.321 0.318 0.132
39 0.235 0.244 0.240 0.054
0 0.183 0.188 0.186  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CYP2E1 ELISA Kit was designed for the quantitative measurement of Mouse CYP2E1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL.

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Target Background

Function
(From Uniprot)
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
Gene References into Functions
  1. these results demonstrate that CYP2E1 is important in fast food-mediated liver fibrosis by promoting nitroxidative and endoplasmic reticulum stress, endotoxemia, inflammation, insulin resistance, and low energy expenditure PMID: 28051126
  2. The significant increase of Cyp2e1 transcript level and protein expression was found in the liver and hippocampi during spontaneous recurrent seizures in mice. PMID: 29125184
  3. CYP2E1-induced oxidative stress may be responsible for ethanol-induced suppression of Akt phosphorylation and pharmacological modulation of Akt in liver may be an effective strategy for the treatment of ethanol-induced fatty liver. PMID: 28987868
  4. BI-1 protects against obesity-induced hepatic insulin resistance by regulating CYP2E1 activity and reactive oxygen species production. PMID: 27576594
  5. Baicalin inhibited ethanol-induced expression of reactive oxygen species (ROS) generating enzymes NOX2, p67phox, xanthine oxidase, and iNOS in addition to CYP2E1 activities. PMID: 28951767
  6. Methodology to assay CYP2E1 mixed function oxidase catalytic activity and its induction/ PMID: 25454746
  7. we show that TRPV4 is activated both by damage associated molecular pattern HMGB1 and collagen in diseased Kupffer cells that in turn activate the endothelial NOS (NOS3) to release nitric oxide (NO). The diffusible NO acts in a paracrine fashion in neighboring hepatocytes to deactivate the redox toxicity induced by CYP2E1 PMID: 27913210
  8. Data, including data from studies in knockout mice, suggest that the oxidative metabolism of 1,2-dichloropropane, a carcinogenic solvent/insecticide, is exclusively catalyzed by Cyp2e1; this biotransformation step is indispensable for manifestation of hepatotoxic effect of the solvent. PMID: 25681370
  9. Taken together, these results suggested that 1,2-dichloroethane (1,2-DCE) could enhance CYP2E1 protein expression and enzymatic activity, which could cause oxidative damage in liver, serving as an important mechanism underlying 1,2-DCE-induced liver damage PMID: 25926354
  10. CYP2E1 is important in causing aging-dependent hepatic steatosis, apoptosis and fibrosis possibly through increasing nitroxidative stress. PMID: 26703967
  11. Our results support a significant role for CYP2E1 as a novel 4-Aminobiphenyl N-oxidizing enzyme in adult mice PMID: 25922528
  12. Schisandrol B protects against APAP-induced liver injury, potentially through inhibition of CYP2E1/3A11-mediated APAP bioactivation and regulation of the p53, p21, CCND1, PCNA, and BCL-2 to promote liver regeneration. PMID: 25319358
  13. Studied hepatic stellate cell cytoglobin involvement in acetaminophen induced hepatotoxicity through the regulation of CYP2E1. PMID: 25686096
  14. Data suggest that expression of Cyp2e1 in adipose tissue can be regulated by dietary factors; here, expression of Cyp2e1 in white adipose tissue is down-regulated in obesity caused by high-fat diet. PMID: 25744306
  15. The Cyp2e1-null mice displayed a susceptibility to lung toxicity of styrene similar to that of the wild-type animals; however, Cyp2f2-null mice were resistant to styrene-induced pulmonary toxicity. PMID: 24320693
  16. In mice that lacked CYP2E1, there was no increase in inflammation mediators in environment-linked nonalcoholic steatohepatitis. PMID: 24211274
  17. Butadiene metabolites reversibly, but not irreversibly, inhibit CYP2E1 metabolic activity; no difference in butadiene metabolite inhibition of rat and mouse CYP2E1 activity PMID: 24021170
  18. It was concluded that these data support a novel role for intestinal CYP2E1 in alcohol-induced intestinal hyperpermeability via a mechanism involving CYP2E1-dependent induction of oxidative stress and upregulation of circadian clock proteins. PMID: 23660503
  19. Female steroid hormones are clearly involved in the regulation of CYP2E1. PMID: 23548611
  20. The results show that acute alcohol elevation of CYP2E1, oxidative stress, and activation of JNK interact to lower autophagy and increase lipogenic SREBP resulting in fatty liver. PMID: 22749809
  21. CYP2E1 plays an important role in the advancement of steatotic livers to inflammatory non-alcoholic steatohepatitis and insulin resistance development. PMID: 22668639
  22. Cytoplasmic HSP90 and membrane-bound CYP2E1 may directly interact with each other as partner proteins, leading to the dissociation of the CYP2E1 from the membrane. PMID: 23241420
  23. Inhibition of CYP2E1 attenuates chronic alcohol intake-induced myocardial contractile dysfunction and apoptosis. PMID: 22967841
  24. while CYP2E1 is not involved in isoniazid-induced hepatotoxicity, while an isoniazid metabolite might play a role in isoniazid-induced cholestasis through enhancement of bile acid accumulation and mitochondria beta-oxidation. PMID: 23142471
  25. Inhibition of autophagy promotes binge ethanol induced hepatotoxicity, steatosis and oxidant stress via CYP2E1. PMID: 22819980
  26. CYP2E1 contributes to ethanol-induced and obesity-induced oxidant stress and liver injury. PMID: 23104821
  27. Data sugget that knockdown or downregulation of CYP2E1 might be a therapeutic strategy to control the development of dilated cardiomyopathy (DCM) after mutations of cTnT(R141W) or other factors. PMID: 22665122
  28. Data from CYP2E1 knockout mice suggest that CYP2E1 is involved in glucose homeostasis; knockout of CYP2E1 prevents development of high-fat diet-induced obesity, insulin resistance, glucose intolerance, hyperlipidemia, and fatty liver. PMID: 22185839
  29. Alpha-lipoic acid administration attenuated methionine choline deficient diet-induced steatohepatitis in C57BL/6 mice and reduced expression of CYP2E1. PMID: 22154902
  30. results demonstrate that CYP2E1 over-expression produces apoptosis and that the up-regulation of CYP2E1 in cTnT(R141W) transgenic mice also correlates with apoptosis in this model PMID: 21352494
  31. CYP2E1 plays a major role in ethanol-induced fatty liver and oxidant stress. PMID: 21525766
  32. Hepatocyte-specific CYP2E1 overexpression results in increased oxidative stress and nitrosative stress. PMID: 20594230
  33. The up-regulation of CYP2E1 induced by microcystin LR was inhibited by epigallocatechin-3-gallate. PMID: 20388359
  34. Alcohol increased the activity of CYP2E1 and micronucleus formation rate in mice with benzene poisoning. PMID: 20137700
  35. taurine can reduce the activity and protein level of CYP2E1 enzyme, inhibiting the activation of JNK PMID: 20166895
  36. Trans-resveratrol and tannic acid may modulate cytochrome P450 2E1 and influence the metabolic activation of xenobiotics mediated by this P450 isoform. PMID: 12545198
  37. CYP2E1, not esterase, is the principal enzyme responsible for urethane metabolism PMID: 12704224
  38. Important role in modulating CYP2E1-mediated toxicity in HepG2 cells by regulating CYP2E1 levels and by removal of oxidized proteins. May be important in CYP2E1-catalyzed toxicity of hepatotoxins and in alcohol-induced liver injury. PMID: 12774019
  39. P450 2E1 has a role in inducing heme oxygenase-1 through ERK MAPK pathway PMID: 12777398
  40. CYP2E1 expression is regulated by histidine decarboxylase PMID: 14642533
  41. This study indicates that the occurrence of CYP 2E1 in C57/bl mouse brain is relevant to MPTP toxicity, and suggests that this isozyme may have a detoxificant role related to the efflux transporter of the toxin PMID: 15447662
  42. increased hepatocyte CYP2E1 expression and the presence of steatohepatitis result in the down-regulation of insulin signaling PMID: 15632182
  43. Ethanol increases mitochondrial Cyp2e1 in mouse liver. PMID: 16337197
  44. Nrf2 plays a key role in the adaptive response against increased oxidative stress caused by CYP2E1. PMID: 16374848
  45. Nrf2, through up-regulation of glutamate-cysteine ligase and increase of GSH levels, protects against CYP2E1-dependent AA toxicity PMID: 16551616
  46. The unusually rapid carbon monoxide binding kinetics of P450 2E1 indicate that it is more dynamically mobile than other P450s and thus able to more readily interconvert among alternate conformations. PMID: 17176083
  47. CYP3A and CYP2E1 contribute to acetaminophen hepatotoxicity in alcohol-treated mice. PMID: 17392391
  48. obesity contributes to oxidative stress and liver injury and that induction of CYP2E1 enhances these effects PMID: 17538970
  49. Finds oxidative DNA damage more intense in Aldh2 -/- mice than in Aldh2 +/+ mice suggesting that ALDH2-deficient individuals may be more susceptible than wild-type ALDH2 individuals to ethanol-mediated liver disease, including cancer. PMID: 17951967
  50. Single-dose ethanol administration downregulates the expression of cytochrome p450 2E1 mRNA in the presence of inactive ALDH2. PMID: 17980998

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Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.
Database Links
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