Mouse Dickkopf-related protein 3(DKK3) ELISA kit

Code CSB-EL006922MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9QUN9
Lead Time 5-7 working days
Abbreviation DKK3
Protein Biological Process 1 Signaling Pathway
Target Name dickkopf homolog 3 (Xenopus laevis)
Alias REIC, RIG, RIG-like 5-6|RIG-like 7-1|dickkopf 3|dickkopf homolog 3|regulated in glioma
Species Mus musculus (Mouse)
Protein Biological Process 3 Wnt signaling pathway
Sample Types serum, plasma, tissue homogenates
Detection Range 9.4 pg/mL-600 pg/mL
Sensitivity 2.35 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.
HGNC 2893
RGD 621846
MGI 1354952
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse DKK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-95
1:2Average %101
Range %97-104
1:4Average %83
Range %80-86
1:8Average %104
Range %100-109
Recovery
The recovery of mouse DKK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-96
EDTA plasma (n=4)9387-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
6002.289 2.487 2.388 2.204
3001.582 1.532 1.557 1.373
1500.881 0.898 0.890 0.706
750.530 0.502 0.516 0.332
37.50.382 0.375 0.379 0.195
18.80.312 0.306 0.309 0.125
9.40.251 0.248 0.250 0.066
00.182 0.186 0.184
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Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
Subcellular Location Secreted
Protein Families Dickkopf family
Tissue Specificity Highest expression in brain, eye and heart.
Database Links

KEGG: mmu:50781

STRING: 10090.ENSMUSP00000033036

UniGene: Mm.55143

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