Product Details

Target Name

ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)

Alternative Names

Fcn2 ELISA Kit; FcnbFicolin-2 ELISA Kit; Collagen/fibrinogen domain-containing protein 2 ELISA Kit; Ficolin-B ELISA Kit; Ficolin-beta ELISA Kit; L-ficolin ELISA Kit

Abbreviation

FCN2

Uniprot No.

O70497

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	92
	Range %	88-96
1:2000	Average %	94
	Range %	90-98
1:4000	Average %	95
	Range %	90-100
1:8000	Average %	88
	Range %	83-93

Recovery

The recovery of mouse FCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-89
EDTA plasma (n=4)	96	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.495	2.569	2.532	2.385
4000	2.014	2.071	2.043	1.896
2000	1.622	1.584	1.603	1.456
1000	1.178	1.113	1.146	0.999
500	0.731	0.792	0.762	0.615
250	0.456	0.428	0.442	0.295
125	0.345	0.327	0.336	0.189
0	0.147	0.146	0.147

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse FCN2 ELISA Kit was designed for the quantitative measurement of Mouse FCN2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

Target Background

Function
(From Uniprot)

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin.

Gene References into Functions

this study shows that FCN B plays an important role in the development of collagen Ab-induced arthritis PMID: 28739878
Ficolin-2 defends against virulent Mycobacteria tuberculosis infection in vivo, and its insufficiency is associated with infection in humans. PMID: 24040095
Data show that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells. PMID: 23911405
These results identify mouse ficolin-B as a functional member of the ficolin family activating complement via the lectin pathway. PMID: 22459270
Results show that ficolin B in mouse bone marrow is an oligomeric protein. Ficolin B contained very low, but detectable, amounts of MASP-2 and sMAP and showed detectable C4-deposition activity on immobilized N-acetylglucosamine. PMID: 22523468
Ficolin B exhibited a distinct ontogeny pattern that switched from embryonic liver to postnatal bone marrow and spleen. The cells that express ficolin B mRNA were identified as belonging to the myeloid cell lineage. PMID: 15950722
Ficolin B showed multimeric structures and revealed binding to both N-acetylglucosamine and N-acetylgalactosamine. Ficolin B specifically recognized sialic acid residues. Ficolin B plays a distinct role through its unique carbohydrate-binding specificity. PMID: 16328467

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Subcellular Location

Secreted.

Protein Families

Ficolin lectin family

Database Links

KEGG: mmu:14134

STRING: 10090.ENSMUSP00000028179

UniGene: Mm.182935

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Code	CSB-EL008551MO
Size	96T,5×96T,10×96T
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Mouse Ficolin-2(FCN2) ELISA kit

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