Mouse Ficolin-2(FCN2) ELISA kit

Code CSB-EL008551MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
Alternative Names Fcn2 ELISA Kit; FcnbFicolin-2 ELISA Kit; Collagen/fibrinogen domain-containing protein 2 ELISA Kit; Ficolin-B ELISA Kit; Ficolin-beta ELISA Kit; L-ficolin ELISA Kit
Abbreviation FCN2
Uniprot No. O70497
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 92
Range % 88-96
1:2000 Average % 94
Range % 90-98
1:4000 Average % 95
Range % 90-100
1:8000 Average % 88
Range % 83-93
The recovery of mouse FCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 84 80-89
EDTA plasma (n=4) 96 92-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
8000 2.495 2.569 2.532 2.385
4000 2.014 2.071 2.043 1.896
2000 1.622 1.584 1.603 1.456
1000 1.178 1.113 1.146 0.999
500 0.731 0.792 0.762 0.615
250 0.456 0.428 0.442 0.295
125 0.345 0.327 0.336 0.189
0 0.147 0.146 0.147  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

(From Uniprot)
May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin (By similarity).
Gene References into Functions
  1. this study shows that FCN B plays an important role in the development of collagen Ab-induced arthritis PMID: 28739878
  2. Ficolin-2 defends against virulent Mycobacteria tuberculosis infection in vivo, and its insufficiency is associated with infection in humans. PMID: 24040095
  3. Data show that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells. PMID: 23911405
  4. These results identify mouse ficolin-B as a functional member of the ficolin family activating complement via the lectin pathway. PMID: 22459270
  5. Results show that ficolin B in mouse bone marrow is an oligomeric protein. Ficolin B contained very low, but detectable, amounts of MASP-2 and sMAP and showed detectable C4-deposition activity on immobilized N-acetylglucosamine. PMID: 22523468
  6. Ficolin B exhibited a distinct ontogeny pattern that switched from embryonic liver to postnatal bone marrow and spleen. The cells that express ficolin B mRNA were identified as belonging to the myeloid cell lineage. PMID: 15950722
  7. Ficolin B showed multimeric structures and revealed binding to both N-acetylglucosamine and N-acetylgalactosamine. Ficolin B specifically recognized sialic acid residues. Ficolin B plays a distinct role through its unique carbohydrate-binding specificity. PMID: 16328467

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Subcellular Location Secreted
Protein Families Ficolin lectin family
Database Links

KEGG: mmu:14134

STRING: 10090.ENSMUSP00000028179

UniGene: Mm.182935


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