Mouse Interleukin 18,IL-18 ELISA Kit

Code CSB-E04609m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 18 (interferon-gamma-inducing factor)
Alternative Names Il18 ELISA Kit; Igif ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
Abbreviation IL18
Uniprot No. P70380
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 82-94  
1:2 Average % 97  
Range % 91-103  
1:4 Average % 93  
Range % 88-99  
1:8 Average % 94  
Range % 90-100  
The recovery of mouse IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-100  
EDTA plasma (n=4) 99 94-107  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.646 2.552 2.599 2.484  
50 2.218 2.186 2.202 2.087  
25 1.874 1.804 1.839 1.724  
12.5 1.332 1.329 1.331 1.216  
6.25 0.925 0.901 0.913 0.798  
3.12 0.527 0.499 0.513 0.398  
1.56 0.261 0.278 0.270 0.155  
0 0.119 0.111 0.115    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Q&A and Customer Reviews

 Customer Reviews
  • Sample type: Lung tissues
    Sample species: Mouse
    Review: IL-1β and IL-18 level in lung tissues measured by ELISA. Each bar represents mean ± SEM (n = 6). *p < 0.05, **p < 0.01 versus sham mice; #p < 0.05, ##p < 0.01 versus LPS-induced lung injury mice.
    PMID: 30126430
  • Sample type: Plasma
    Sample species: Mouse
    Review: Effect of GAL (4 mg/kg; i.p) and/or MLA (5.6 mg/kg; i.p) on plasma levels of [a] Cr, [b] Cys-C and [c] IL-18, as well as renal [d] NGAL content in ZYM-induced AKI. Data are expressed as mean of 6 mice ± SD. Statistical analysis was performed using one way analysis of variance (ANOVA) followed by Tukey’s Multiple Comparison test; as compared to CONT(*), ZYM (@), and ZYM + GAL (#) groups, P < 0.05. GAL was injected 1 and 6 h post ZYM challenge, while MLA was administered 15 min before GAL. AKI: acute kidney injury; CONT: control; Cr: creatinine; Cys-C: cystatin C; GAL: galantamine; MLA: methyllycaconitine, IL-18: interleukin-18; NGAL: neutrophil gelatinase-associated lipocalin; ZYM: zymosan.
    PMID: 30429582
  • Sample type: Cell culture supernatant
    Sample species: Mouse
    Review: ELISA was used to determine (A) the levels of IL-1β, (B) IL-18 and (C) TNFα in hippocampus. ELISA showed that the level of IL-1β, IL-18 and TNFα in hippocampus on 24h after LPS injection was significantly increased in the LPS group, but reversed in the LPS mice pretreated with Crocin, Data are presented as mean ± SEM, n = 10 mice/group, ##p < 0.01 versus the control group, *P<0.05 and **P<0.01 versus the LPS group.
    PMID: 30179677
  • Sample type: Serum or liver
    Sample species: Mouse
    Review: The levels of IL-18, iNOS, MCP-1, MIP-1β, and RANTES in serum or liver were detected by using mouse ELISA kits.
    PMID: 30032072
  • Sample type: Bronchoalveolar lavage fluid
    Sample species: Mouse
    Review: Productions of IL-6, IL-1 β, IL-18, and TNF- α in BALF. Data were presented as means ± SEM (n = 6 in each group). *P < 0.05 **P < 0.001. ***P < 0.001.
    PMID: 30373775

Target Data

Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Gene References into Functions
  1. This study identifies a role for IL18 in suppressing aberrant neuronal transmission in AD. PMID: 30127003
  2. Chimeric antigen receptor T cells releasing IL-18 convert to T-Bet(high) FoxO1(low) effectors that exhibit augmented activity against advanced solid tumors. PMID: 29241547
  3. The data suggest that inflammasome signaling is largely protective during murine coronavirus infection, in large part due to the pro-inflammatory effects of IL-18. PMID: 28895072
  4. downstream mediator of IL18 receptor activation, phospho-NF-kB, was increased in basolateral amygdala neurons expressing IL18 receptors PMID: 27590137
  5. This study provides the first evidence of the ability of IL-18 to induce B-type natriuretic peptide synthesis in vitro and outlines the relationship between the two molecules in acute HF patients with an ongoing inflammatory status. PMID: 24585936
  6. Interleukin-18 plays a role in advancing sepsis-induced cardiac dysfunction through inhibition of PP2A activity. PMID: 28526544
  7. These observations suggest that IL-18 exerts direct effects upon the GnRH neuron via IL-18Ralpha and acts on GnRH neurons through an autocrine or paracrine pathway. PMID: 28373090
  8. Reduced IL-18 serum concentration in children after HUS with no difference in its urine concentration may indicate a loss of the protective effects of this cytokine on renal function due to previously occurred HUS. PMID: 27982687
  9. In conclusion, liver X receptor activation inhibits IL-18 production through regulation of its transcription and maturation into an active pro-inflammatory cytokine. PMID: 27149934
  10. MyD88 signaling in myeloid and dendritic cells is dispensable for IFN-gamma-dependent control of type A F. tularensis infection. PMID: 28951422
  11. study found that IL-18 and IL-1beta are differentially regulated. Despite being constitutively expressed, IL-18 expression was increased and sustained after stimulation of TLRs. In contrast, IL-1beta was induced but not sustained after chronic treatment. PMID: 28468974
  12. This provides new insight into the immune phenotype, mechanisms, and signaling pathways that operate in microglial neurotoxic activation in amyotrophic lateral sclerosis. PMID: 28336525
  13. the GLA-SE adjuvant operates through interaction with IL-18-producing SCMsmall ef, Cyrillic for the rapid induction of B cell expansion and differentiation, Ab secretion, and Th1 responses PMID: 27794001
  14. IL-18 is essentially involved in mediating C. jejuni infection in the gnotobiotic mouse model. PMID: 27322540
  15. These results demonstrate a central role for the AIM2 inflammasome in preventing dysbiosis and intestinal inflammation through regulation of the IL-18/IL-22BP/IL-22 and STAT3 pathway PMID: 27524110
  16. Aldosterone induced IL-18 gene expression in renal tubular epithelial cells in a concentration- and time-dependent manner. PMID: 27413021
  17. this study demonstrated the critical function of IL-18 in lipid metabolism and these findings might contribute to the progress of novel treatments for nonalcoholic fatty liver disease or nonalcoholic steatohepatitis. PMID: 27063959
  18. Results indicated that IL-18 has roles apart from those as a proinflammatory cytokine in cardiac myocytes and suggested that IL-18 contributes to the homeostatic maintenance of mitochondrial function and gap-junction turnover in cardiac myocytes, possibly by upregulating autophagy. PMID: 27288439
  19. IL-18-elicited NK cell perforin responses seem to be critical for coordinating mucosal inflammation during early infection PMID: 27341123
  20. Data indicate that NLRC4 activation in Intestinal epithelial cells (IECs) leads to cell expulsion and IL-18 release, and implicate Caspase-8 in NLRC4 inflammasome responses in vivo by generation of doubly deficient in Caspase-1 and Caspase-8. PMID: 28410991
  21. this study shows that IL-18, cooperatively with IL-23, induced prominent inflammation and enhanced psoriasis-like epidermal hyperplasia PMID: 28299442
  22. telmisartan can reduce traumatic cerebral edema by inhibiting the NLRP3 inflammasome-regulated IL-1beta and IL-18 accumulation. PMID: 27465336
  23. Data, including data from studies in knockout mice, suggest that TNF (tumor necrosis factor), TNFR1 (TNF receptor 1), and IL18 (interleukin 18) are involved in liver lipid metabolism. IL18 or TNFR1 knockout mice on chow show higher liver triglyceride deposition than wild-type mice fed chow; in IL18 or TNFR1 knockout mice, high-refined-carbohydrate diet does not lead to fatty liver disease as it does in wild-type mice. PMID: 27863204
  24. GABA-B receptor antagonists reduced Il18 levels in male mice and increased Il18 levels in female mice following neonatal hypoxia ischemia. PMID: 27731803
  25. NLRP1 is an innate immune sensor that functions in the context of metabolic stress to produce IL-18, preventing obesity and metabolic syndrome. PMID: 26603191
  26. an important role in iNKT cell-mediated experimental eosinophilic esophagitis PMID: 25801352
  27. IL-18 is sufficient to protect Birc3-deficient mice from sodium dodecyl sulfate-induced colitis. PMID: 26037070
  28. IL-18 plays a facilitative role via NF-kappaB activation in pulmonary hypertension formation. PMID: 26440469
  29. IL-18 is a key epithelial-derived cytokine that differentially regulates distinct subsets of intestinal CD4(+) T cells during both homeostatic and inflammatory conditions PMID: 25736457
  30. Genetic ablation of IL-18 does not protect mice against maladaptive right ventricular remodeling following exposure to hypobaric hypoxia. PMID: 26747780
  31. We conclude that in the systemic lupus erythematosus syndrom IL-18 is involved specifically in the renal pathogenesis PMID: 26465326
  32. These results uncover the direct role of IL-18 in promoting goblet cell dysfunction during colitis, leading to breakdown of the mucosal barrier. PMID: 26638073
  33. IL-18 is a potent stimulator of Chi3l1 and that Chi3l1 is an important mediator of IL-18-induced inflammatory, fibrotic, alveolar remodeling, and cytotoxic responses. PMID: 25955511
  34. macrophages are not involved in IL-18-mediated susceptibility to L. amazonensis. PMID: 26009021
  35. The published data prompt to the hypothesis that IL-18 induces a broad spectrum of COPD-like inflammatory and remodeling responses in the murine lung PMID: 25922275
  36. Following hypoxic ischemic insult, long term creatine monohydrate supplementation up regulates the IL-6 and IL-18 concentrations triggering the neuroinflammatory and neuroprotective responses. PMID: 26639507
  37. High and fluctuating levels of glucose may be associated with inflammation and diabetic atherosclerosis by regulating the expression levels of IL-18. PMID: 25955000
  38. a new mechanism by which PKC-beta activation promotes EC dysfunction caused by the de-regulation of the IL-18/IL-18BP pathway, leading to increased VCAM-1 expression, monocyte/macrophage adhesion, and accelerated atherosclerotic plaque formation in diabetes PMID: 25808972
  39. PMA treatment during a vulnerable period can alter brain development. IL-18 and IRAK-4 appear to be important for the development of PMA induced injury. PMID: 25918710
  40. IL-18 levels modulate innate immune responses and cryptosporidiosis in mice. PMID: 25155632
  41. these results indicate that the TLR2/NLRP3/CASP1/IL-18 axis is critical to H. pylori-specific immune regulation. PMID: 26214524
  42. This study identifies NCC as an IL18-binding protein that collaborates with IL18r in cell signaling, inflammatory molecule expression, and experimental atherogenesis. PMID: 26099046
  43. Neutralization of IL-18 during alveolar hypoxia improves left ventricle diastolic function and partly prevents right ventricle hypertrophy PMID: 25182570
  44. This study reveals essential role of autophagy as a negative regulator of lung inflammation and identifies IL-18 as a critical mediator in lung injury due to autophagy deficiency. PMID: 25888640
  45. IL-18, but not IL-1beta has a role in aberrant actin depolymerization that triggers the pyrin inflammasome and autoinflammatory disease PMID: 26008898
  46. findings show how TLR2 deficiency accelerates IL18-mediated immunosuppression during liver carcinogenesis PMID: 25600646
  47. Nlrp3 inflammasome is essential for resistance against P. brasiliensis because it orchestrates robust caspase-1 activation and triggers an IL-18-dependent proinflammatory response. PMID: 25825440
  48. IL-18 can positively impact bone marrow lymphopoiesis and T cell development, presumably via interaction with the c-Kit and IL-7 signaling axis. PMID: 25780034
  49. Alprostadil treatment can protect renal function by reducing proteinuria. These effects are mediated, at least in part, through down-regulation of Ang-2 and IL-18 expression PMID: 25200363
  50. IL-18 was required for the expression of IL-22 in innate lymphoid cells upon Toxoplasma gondii infection. PMID: 25680273
  51. we demonstrate that NK cells require IL-18 signaling to generate a robust primary response during mouse CMV (MCMV) infection but do not require this signal for memory cell maintenance or recall responses PMID: 25589075
  52. Data demonstrate that IL-18 can act directly on neuronal cells affecting the STAT3 pathway; therefore, possibly regulating the expression of specific genes within the hippocampus. PMID: 24603356
  53. IL-1 and IL-18 are pivotal for chemotherapy-induced mucositis. PMID: 24952429
  54. these results suggest that ASC plays a detrimental role in lethal L. monocytogenes infection through IL-18 production in an inflammasome-dependent and -independent manner. PMID: 25251560
  55. IL-18 contributes to inducing IFN-gamma in an immunosuppressive cutaneous environment caused by viral oncogene-driven hyperplasia. PMID: 24756108
  56. our data suggest that IL-18 produced during S. mansoni infection play a role in the expansion of multiple cytokine producing hepatic t-cells. PMID: 24824897
  57. IL-18-deficient mice were partially resistant to behavioural impairments induced by pneumococcal meningitis; IL-18 deficiency and consequent reduced immune response did not improve cognitive impairment in wild-type mice following pneumococcal meningitis PMID: 24503119
  58. data reveal a previously unrecognized role for NK cell-extrinsic IL-18 signaling in NK-cell activation through upregulation of NKG2D ligands. PMID: 24846540
  59. induction of IFN-gamma because of IL-18 expression resulted in a recombinant vaccinia virus that replicated to low but detectable levels in vivo, yet elicited slightly better Cytotoxic T-cell and anamnestic humoral immune responses. PMID: 24168450
  60. The loss-of-function results support the hypothesis that endogenous IL-18 suppresses appetite and promote energy expenditure and lipid fuel substrate utilization not only during sickness, but also in healthy adults consuming high-fat diets. PMID: 24316258
  61. Increased IL-18 in the gingival tissues evokes chronic periodontitis after bacterial infection, presumably via a T cell-mediated pathway. PMID: 24646956
  62. IL-18 expression is increased in murine vein grafts. Necrotic VSMCs promote monocytic inflammasome activation via an IL-1beta/IL-18-dependent pathway at the early stage of vein graft remodeling. PMID: 25012128
  63. Study suggests that the lower level of IL-18 in caspase-1-/- mice might be causing obesity development similarly to IL-18-deficient mice. PMID: 23689355
  64. In our mouse model, fatal progression of AIH is mediated by IL-18-dependent differentiation of T cells into Th1 cells and effector T cells, respectively, and that CXCR3-CXCL9 axis-dependent migration of those T cells is crucial for fatal progression. PMID: 24700550
  65. tumor-derived IL-18 plays an important role in the phagocytosis of macrophages and IL-18-stimulated macrophages may damage tumor endothelial cells PMID: 24286318
  66. VEGF and IL-18 suppress each other's production and effects on the vasculature suggesting that IL-18 may provide benefit in multiple retinal/choroidal vascular diseases. PMID: 24515951
  67. Data indicate that both inflammasome-related and -unrelated pathways contributed to IL-18 dependence in vivo following ISCOMATRIX adjuvant administration. PMID: 24610009
  68. IL-1 induces a release of active IL-18 in that mediates LV systolic dysfunction but not the induction of IL-6. PMID: 24531812
  69. IL-18 and IL-13 play important roles in the pathogenesis of comorbidity in a chronic obstructive pulmonary disease mice model. PMID: 24565845
  70. The detrimental role of IL-1beta and IL-18 was confirmed in mice models of septic shock. Deficiency of both IL-1beta and IL-18 additively prevented sepsis-induced mortality. PMID: 24456467
  71. A stepwise cascade of hierarchized molecules heterogeneously made and used during melanoma response to IL-18, induced hydrogen peroxide (H2O2), in turn activating VLA-4 and melanoma cell adhesion to endothelium. PMID: 23938462
  72. Lines harboring cryopyrin-associated periodic syndromes (CAPS)-associated mutations in Nlrp3 have elevated levels of IL-1beta and IL-18 and closely mimic human disease. PMID: 24084736
  73. IL-18 induces profibrotic cellular changes and collagen production in renal tubular epithelial cells via STAT3 activation. PMID: 23904224
  74. Data suggest IL18 is important factor in the induction of insulin resistance in hyperthyroidism; in experimental hyperthyroid mice, serum IL18 levels are elevated and insulin resistance increases; insulin resistance is induced in IL18-injected mice. PMID: 23257837
  75. IL-18 production and activation during renal obstruction is dependent on intact TLR4 signaling. PMID: 23260234
  76. These results demonstrate that renal tubular epithelial cells are the primary source of IL-18 production during obstructive injury, and that tubular cell production of IL-18 occurs independent of macrophage infiltration. PMID: 23077611
  77. Absence of Krt1 caused a prenatal increase in interleukin-18 (IL-18) and the S100A8 and S100A9 proteins, accompanied by a barrier defect and perinatal lethality. PMID: 23132931
  78. The effect of L. lactis subsp. cremoris on interferon-gamma expression in NK and T cells via the IL-12 and IL-18 pathway is described. PMID: 23102661
  79. Mycobacterium tuberculosis infection of dendritic cells leads to partially caspase-1/11-independent IL-1beta and IL-18 secretion but not to pyroptosis. PMID: 22911706
  80. Fas (TNF receptor superfamily member CD95)-induced IL-1beta and IL-18 processing is caspase-8 dependent and RIP3 independent PMID: 23144495
  81. deletion of the viral IL-18 bp gene increases MVA vaccine efficacy PMID: 22384183
  82. These studies define the spectrum of inflammatory, parenchymal, airway, and vascular alterations that are induced by pulmonary IL-18; highlight the similarities between these responses and the lesions in COPD PMID: 22383501
  83. IL-18 produced by tumor cells elicits Kit(+)CD11b(-) natural killer cells endowed with B7-H1-dependent immunoablative functions in mice. PMID: 22427351
  84. Rapid and specific release of IL-18 from Alternaria-exposed damaged airway epithelial cells can directly initiate Th2 differentiation of naive CD4(+) T-cells via a unique NF-kappaB dependent pathway PMID: 22347372
  85. NLRP3 has a protective role in age-related macular degeneration through the induction of IL-18 by drusen components. PMID: 22484808
  86. NLRC4 regulates pyroptosis while NLRP3 regulates production of protective IL-18 and deleterious IL-1beta. PMID: 22241982
  87. tolerogenic reprogramming of DCs ensures the persistence of H. pylori and protects against allergic asthma in a process that requires IL-18 PMID: 22307326
  88. the inflammatory cytokine IL-18 induces self-reactive innate antibody responses regulated by natural killer T cells PMID: 22135456
  89. Antroquinonol may have therapeutic potential for the early treatment of ASLN via its differential regulation of T cell function and lowering of IL-18 production, but also via the promotion of Nrf2 activation. PMID: 21905011
  90. we examined the role of inflammasome-driven IL-18 activation in the development of Systemic lupus erythematosus endothelial progenitor cells and circulating angiogenic cells dysfunction PMID: 22058412
  91. These results reveal novel requirements for IL-18 in innate immune cell homeostasis and activation. PMID: 21263463
  92. NK1.1(+) gammadeltaT cells function as inflammatory mediators in the early phase of IL-18/IL-2-induced interstitial lung disease PMID: 21257923
  93. A critical role is established for IL-18/IL- 18 receptor alpha in the maturation of dendritic cells, cytokine production by natural killer (NK)T and NK cells, and induction of pathogenic tumor necrosis factor(TNF)-alpha-producing CD8+ T cells. PMID: 21715688
  94. caspase-1 activation of interleukin-1beta and IL-18, which is associated with the inflammasome pathway, does not contribute to P. berghei ANKA-induced immunopathology. PMID: 21708993
  95. IL-18-mediated, nitric oxide-induced apoptosis in TNF-alpha-mediated osteoclastogenesis of bone marrow cells. PMID: 21611811
  96. results define IL-18 as an immunosuppressive cytokine in cancer. Our findings suggest novel clinical implementations of anti-PD-1 antibodies in human malignancies that produce IL-18 PMID: 21724589
  97. MiR-146a mimics suppressed IL-18 expression (P<0.05), and miR-146a specific inhibitor increased IL-18 expression significantly PMID: 21557898
  98. IL-18 is required for self-reactive T cell expansion in NOD mice. PMID: 21414755
  99. The authors suggest that MyD88 functions to support T-cell-specific IL-18 receptor signaling, which in turn is essential for the formation of adaptive immunity to fowlpox virus -encoded chicken ovalbumin. PMID: 21248035
  100. Circulating IL-18 levels were elevated in Stat4-deficient compared to Stat4-intact mice, suggesting that this interleukin might contribute to the progression of lupus nephritis independent of Stat4. PMID: 20980973

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Subcellular Location Secreted
Protein Families IL-1 family
Database Links

KEGG: mmu:16173

STRING: 10090.ENSMUSP00000137193

UniGene: Mm.1410

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