Mouse Interleukin 18,IL-18 ELISA Kit

Instructions
Code CSB-E04609m
Size 96T,5×96T,10×96T

Product Details

Target Name interleukin 18 (interferon-gamma-inducing factor)
Alternative Names IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma|interferon-gamma-inducing factor|interleukin 18|interleukin-1 gamma|interleukin-18
Abbreviation IL18
Uniprot No. P70380
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 82-94  
1:2 Average % 97  
Range % 91-103  
1:4 Average % 93  
Range % 88-99  
1:8 Average % 94  
Range % 90-100  
Recovery
The recovery of mouse IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-100  
EDTA plasma (n=4) 99 94-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.646 2.552 2.599 2.484  
50 2.218 2.186 2.202 2.087  
25 1.874 1.804 1.839 1.724  
12.5 1.332 1.329 1.331 1.216  
6.25 0.925 0.901 0.913 0.798  
3.12 0.527 0.499 0.513 0.398  
1.56 0.261 0.278 0.270 0.155  
0 0.119 0.111 0.115    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Q&A and Customer Reviews

 Customer Reviews
  • Sample type: Lung tissues
    Sample species: Mouse
    Review: IL-1β and IL-18 level in lung tissues measured by ELISA. Each bar represents mean ± SEM (n = 6). *p < 0.05, **p < 0.01 versus sham mice; #p < 0.05, ##p < 0.01 versus LPS-induced lung injury mice.
    PMID: 30126430
  • Sample type: Plasma
    Sample species: Mouse
    Review: Effect of GAL (4 mg/kg; i.p) and/or MLA (5.6 mg/kg; i.p) on plasma levels of [a] Cr, [b] Cys-C and [c] IL-18, as well as renal [d] NGAL content in ZYM-induced AKI. Data are expressed as mean of 6 mice ± SD. Statistical analysis was performed using one way analysis of variance (ANOVA) followed by Tukey’s Multiple Comparison test; as compared to CONT(*), ZYM (@), and ZYM + GAL (#) groups, P < 0.05. GAL was injected 1 and 6 h post ZYM challenge, while MLA was administered 15 min before GAL. AKI: acute kidney injury; CONT: control; Cr: creatinine; Cys-C: cystatin C; GAL: galantamine; MLA: methyllycaconitine, IL-18: interleukin-18; NGAL: neutrophil gelatinase-associated lipocalin; ZYM: zymosan.
    PMID: 30429582
  • Sample type: Cell culture supernatant
    Sample species: Mouse
    Review: ELISA was used to determine (A) the levels of IL-1β, (B) IL-18 and (C) TNFα in hippocampus. ELISA showed that the level of IL-1β, IL-18 and TNFα in hippocampus on 24h after LPS injection was significantly increased in the LPS group, but reversed in the LPS mice pretreated with Crocin, Data are presented as mean ± SEM, n = 10 mice/group, ##p < 0.01 versus the control group, *P<0.05 and **P<0.01 versus the LPS group.
    PMID: 30179677
  • Sample type: Serum or liver
    Sample species: Mouse
    Review: The levels of IL-18, iNOS, MCP-1, MIP-1β, and RANTES in serum or liver were detected by using mouse ELISA kits.
    PMID: 30032072
  • Sample type: Bronchoalveolar lavage fluid
    Sample species: Mouse
    Review: Productions of IL-6, IL-1 β, IL-18, and TNF- α in BALF. Data were presented as means ± SEM (n = 6 in each group). *P < 0.05 **P < 0.001. ***P < 0.001.
    PMID: 30373775

Target Data

Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Subcellular Location Secreted
Protein Families IL-1 family
Database Links

KEGG: mmu:16173

STRING: 10090.ENSMUSP00000137193

UniGene: Mm.1410

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.