Mouse Lactoferrin,LTF/LF ELISA kit

Code CSB-E08833m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
lactotransferrin
Alternative Names
Ltf ELISA Kit; Lactotransferrin ELISA Kit; Lactoferrin ELISA Kit; EC 3.4.21.- ELISA Kit
Abbreviation
LTF
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, breast milk
Detection Range
3.12 μg/mL-200 μg/mL
Sensitivity
3.12 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LTF/LF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %92-106
1:2Average %95
Range %91-99
1:4Average %102
Range %98-107
1:8Average %88
Range %82-93
Recovery
The recovery of mouse LTF/LF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9488-98
EDTA plasma (n=4)10398-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2Average
2000.137 0.138 0.138
1000.225 0.231 0.228
500.393 0.382 0.388
250.668 0.678 0.673
12.51.141 1.136 1.139
6.251.671 1.698 1.685
3.121.915 1.854 1.885
02.121 2.264 2.193
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse LTF ELISA Kit was designed for the quantitative measurement of Mouse LTF protein in serum, plasma, breast milk. It is a Competitive ELISA kit, its detection range is 3.12 μg/mL-200 μg/mL and the sensitivity is 3.12 μg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.; Lactotransferrin is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity. Antimicrobial properties may include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. May have anabolic, differentiating and anti-apoptotic effects on osteoblasts and may also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. May interfere with the lipopolysaccharide (LPS)-stimulated TLR4 signaling.; The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites.
Gene References into Functions
  1. Hepatic Ltf prevents hepatic steatosis through inhibition of dietary lipid uptake in radiation-induced NAFLD mouse model. We also suggest coumestrol as a drug candidate for prevention of NAFLD. PMID: 29975946
  2. Data indicate that lactoferrin uptake across duodenum by glyceraldehyde-3-phosphate dehydrogenase (GAPDH). PMID: 26672975
  3. HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity. PMID: 26148296
  4. Results show that LTF gene expression is downregulated in colorectal cancer tumors and its knock out in mouse model of colitis-associated colorectal dysplasia suggests its protective role in colorectal mucosal immunity and inflammation. PMID: 25057912
  5. Data show that the expression of several genes encoding salivary antimicrobial proteins, such as secretory leukocyte peptidase inhibitor (SLPI), S100A8, and lactotransferrin, was reduced due to myeloid differentiation dactor 88 (MyD88) deficiency. PMID: 25415419
  6. Lactoferrin dampens high-fructose corn syrup-induced hepatic manifestations of the metabolic syndrome in a murine model. PMID: 24816278
  7. We conclude that lactotransferrin represents a late stage differentiation marker of neutrophils, macrophages and distinct subtypes of dendritic cells. PMID: 24561791
  8. The variations in lactoferrin expression levels during developmental stages may be related to its regulatory role in mouse mammary gland immunity. PMID: 25062410
  9. the present work indicated that LF stimulated MC3T3-E1 preosteoblast differentiation mainly through LRP1-independent PKA and p38 signaling pathways. PMID: 24877241
  10. Studie indicate that in iCre knock-in line, the iCre is expressed from endogenous lactoferrin (Ltf) promoter. PMID: 24823394
  11. the homologous LF elicits significant and transient myelopoiesis in experimental mice. PMID: 23879888
  12. administration of lactoferrin led to autopotentiated increased expression of the LTF gene. The decreased lactoferrin mRNA levels in association with obesity and diabetes were replicated in mice adipose tissue PMID: 23333090
  13. The up-regulation of lactorferrin in the brains of the transgenic mouse model of Alzheimer's disease provides evidence of an important role for lactoferrin in alzheimer disease-affected brain tissues PMID: 20599473
  14. Methoxychlor stimulates the mouse lactoferrin gene promoter through a GC-rich element. PMID: 11908639
  15. differential expression and estrogen responsiveness of the lactoferrin gene in different regions of the female reproductive tract PMID: 12390874
  16. these results support the conclusion that lactoferrin does not play a major role in the regulation of iron homeostasis PMID: 12482971
  17. the concentrations of ceruloplasmin and lactoferrin in milk were increased fivefold and more than 38-fold, respectively, within 48 h after weaning PMID: 16989572
  18. These results indicate that although LF deficiency alone is not a primary cause of the defects associated with specific granule deficiency, this protein does play an immunomodulatory role in the oxidative burst response of neutrophils. PMID: 18321995
  19. lactoferrin has multifunctional antiprion activities. PMID: 18717818
  20. This study reports that a novel glycosylphosphatidylinositol (GPI)-anchored lactoferrin CSP82 on uninitiated mouse bone marrow cells may be involved in inducing pro-dendritic cells from common myeloid progenitor. PMID: 19524877

Show More

Hide All

Subcellular Location
Secreted. Cytoplasmic granule.
Protein Families
Transferrin family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1