Mouse Lactoferrin,LTF/LF ELISA kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Alternative Names

Ltf ELISA Kit; Lactotransferrin ELISA Kit; Lactoferrin ELISA Kit; EC 3.4.21.- ELISA Kit

Abbreviation

LTF

Uniprot No.

P08071

Species

Mus musculus (Mouse)

Sample Types

Detection Range

0.156 µg/ml-10 µg/ml

Sensitivity

0.156 µg/ml.

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Competitive

Precision

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µg/ml	OD1	OD2	Average
10	0.145	0.137	0.141
5	0.278	0.267	0.272
2.5	0.531	0.528	0.530
1.25	0.896	0.929	0.913
0.625	1.521	1.501	1.511
0.312	1.801	1.753	1.777
0.156	2.048	2.084	2.066
0	2.440	2.325	2.383

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse LTF ELISA Kit was designed for the quantitative measurement of Mouse LTF protein in serum, plasma, breast milk. It is a Competitive ELISA kit, its detection range is 3.12 μg/mL-200 μg/mL and the sensitivity is 3.12 μg/mL.

Target Background

Function
(From Uniprot)

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.; Lactotransferrin is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity. Antimicrobial properties may include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. May have anabolic, differentiating and anti-apoptotic effects on osteoblasts and may also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. May interfere with the lipopolysaccharide (LPS)-stimulated TLR4 signaling.; The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites.

Gene References into Functions

Hepatic Ltf prevents hepatic steatosis through inhibition of dietary lipid uptake in radiation-induced NAFLD mouse model. We also suggest coumestrol as a drug candidate for prevention of NAFLD. PMID: 29975946
Data indicate that lactoferrin uptake across duodenum by glyceraldehyde-3-phosphate dehydrogenase (GAPDH). PMID: 26672975
HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity. PMID: 26148296
Results show that LTF gene expression is downregulated in colorectal cancer tumors and its knock out in mouse model of colitis-associated colorectal dysplasia suggests its protective role in colorectal mucosal immunity and inflammation. PMID: 25057912
Data show that the expression of several genes encoding salivary antimicrobial proteins, such as secretory leukocyte peptidase inhibitor (SLPI), S100A8, and lactotransferrin, was reduced due to myeloid differentiation dactor 88 (MyD88) deficiency. PMID: 25415419
Lactoferrin dampens high-fructose corn syrup-induced hepatic manifestations of the metabolic syndrome in a murine model. PMID: 24816278
We conclude that lactotransferrin represents a late stage differentiation marker of neutrophils, macrophages and distinct subtypes of dendritic cells. PMID: 24561791
The variations in lactoferrin expression levels during developmental stages may be related to its regulatory role in mouse mammary gland immunity. PMID: 25062410
the present work indicated that LF stimulated MC3T3-E1 preosteoblast differentiation mainly through LRP1-independent PKA and p38 signaling pathways. PMID: 24877241
Studie indicate that in iCre knock-in line, the iCre is expressed from endogenous lactoferrin (Ltf) promoter. PMID: 24823394
the homologous LF elicits significant and transient myelopoiesis in experimental mice. PMID: 23879888
administration of lactoferrin led to autopotentiated increased expression of the LTF gene. The decreased lactoferrin mRNA levels in association with obesity and diabetes were replicated in mice adipose tissue PMID: 23333090
The up-regulation of lactorferrin in the brains of the transgenic mouse model of Alzheimer's disease provides evidence of an important role for lactoferrin in alzheimer disease-affected brain tissues PMID: 20599473
Methoxychlor stimulates the mouse lactoferrin gene promoter through a GC-rich element. PMID: 11908639
differential expression and estrogen responsiveness of the lactoferrin gene in different regions of the female reproductive tract PMID: 12390874
these results support the conclusion that lactoferrin does not play a major role in the regulation of iron homeostasis PMID: 12482971
the concentrations of ceruloplasmin and lactoferrin in milk were increased fivefold and more than 38-fold, respectively, within 48 h after weaning PMID: 16989572
These results indicate that although LF deficiency alone is not a primary cause of the defects associated with specific granule deficiency, this protein does play an immunomodulatory role in the oxidative burst response of neutrophils. PMID: 18321995
lactoferrin has multifunctional antiprion activities. PMID: 18717818
This study reports that a novel glycosylphosphatidylinositol (GPI)-anchored lactoferrin CSP82 on uninitiated mouse bone marrow cells may be involved in inducing pro-dendritic cells from common myeloid progenitor. PMID: 19524877

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Subcellular Location

Secreted. Cytoplasmic granule.

Protein Families

Transferrin family

Database Links

KEGG: mmu:17002

STRING: 10090.ENSMUSP00000035077

UniGene: Mm.282359

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Code	CSB-E08833m
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.