Mouse Leptin,LEP ELISA kit

Instructions
Code CSB-E04650m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name leptin
Alternative Names Lep ELISA Kit; Ob ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
Abbreviation LEP
Uniprot No. P41160
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision   
Linearity    
Recovery   
Typical Data   
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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 Q&A
Q:

Please could you tell us if you have a Mouse Leptin ELISA Kit that can
be used on only 5 ul of serum?

A:
Thanks for your inquiry.
5ul sample is really too little and at least 1:20 dilution is needed if you dilute the sample.
However, for this kit CSB-E04650m, usually there is no need to dilute serum and plasma sample.
So we hope you could try to collect more samples and make the experiment according our manual.

Target Data

Function Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways
Gene References into Functions
  1. In C57bl/6 model, glycaemic index of maternal dietary carbohydrates differentially alters Fto and Lep expression in offspring. PMID: 30241328
  2. A potent, selective, and orally bioavailable inhibitor of the protein-tyrosine phosphatase PTP1B improves insulin and leptin signaling in animal models PMID: 29217773
  3. Catalase overexpression modulates metabolic parameters in a new 'stress-less' leptin-deficient mouse model. PMID: 28645653
  4. Since upregulation of ANGPTL6 is induced on metabolic stress, we investigated the hepatic expression of ANGPTL6 by leptin, a representative adipokine of obesity. Mice on a high-fat diet showed increased serum leptin levels and hepatic Angptl6 expression. PMID: 29852166
  5. This study shows that prolonged exposure to high-fat diets in adulthood is associated with a gradually increasing DNA methylation level at the Leptin and Pparg2 promoters in a depot-specific manner. PMID: 28256596
  6. the study reports a novel mechanistic pathway of leptin-mediated renal inflammation that is dependent on NOX-2-miR21 axis in ectopic manifestations underlying non-alcoholic fatty liver disease-induced co-morbidities PMID: 29660503
  7. These results demonstrated a novel molecular mechanism by which leptin sustained oligodendrocyte precursor cell proliferation and remyelination in a pathological central nervous system. PMID: 28091609
  8. These data demonstrate that FAAH activity is required for leptin's hypophagic effects PMID: 29967158
  9. These data uncouple the mechanisms conferring qualitative and quantitative expression of the leptin gene and further suggest that factor(s) that bind to LepRE1 quantitatively control leptin expression and might be components of a lipid-sensing system in adipocytes. PMID: 29891714
  10. These results indicate that leptin plays a role in nociception induced by acute inflammation PMID: 29204732
  11. results suggest that STAT transcriptional activity is downregulated by high levels of leptin, leading to reduced cAMP-dependent steroidogenic genes (Star and Cyp11a1) expressions in MA-10 Leydig cells. PMID: 28343310
  12. Data suggest that lnc-leptin is among the most prominent lncRNAs expressed in adipocytes; lnc-leptin expression from an enhancer region upstream of leptin is sensitive to insulin and correlates to leptin expression across diverse pathophysiological conditions; induction of lnc-leptin is essential for adipogenesis; its presence is required for maintenance of leptin expression in adipocytes. (lnc = long non-coding RNA) PMID: 29519872
  13. Data (including data from studies using mutant, transgenic, and knockout mice) suggest that gene targets of leptin/leptin-receptor (Lep/Lepr) signaling in hypothalamic neurons regulate energy metabolism; Lep/Lepr signaling appears to up-regulate expression of Atf3 (activating transcription factor-3) in hypothalamic neurons. PMID: 29535089
  14. Study shows that compared to the other mouse lines, db/db mice with dysfunctional leptin receptors had a significantly longer tail flick latency after saline and buprenorphine. The results provide novel support for the interpretation that acute thermal nociception is associated with altered leptin signaling. PMID: 28893589
  15. The studies established a potential link between leptin and adipocyte insulin responsiveness in an NOS2 dependent manner. PMID: 28739528
  16. leptin regulates the expression of osteocalcin in growth plate chondrocytes via the ERK1/2 signaling pathway, while there is no effect on the phosphorylation of either p38 or AKT PMID: 27564111
  17. Hyperleptinemia exacerbates high fat diet-mediated atrial fibrosis and atrial fibrillation. PMID: 28257569
  18. Taken together, these results suggest that leptin, in concert with other stimuli in the micro milieu, may contribute to the development of psoriasis. PMID: 27488462
  19. Thus, Mc4r in the PVH appears to be required for early-life programming of hypertension arising from either maternal obesity or neonatal hyperleptinemia. Early-life exposure of the PVH to maternal obesity through postnatal elevation of leptin may have long-term consequences for cardiovascular health. PMID: 27791019
  20. t the cellular level, leptin promoted effector T-cell responses and facilitated the presentation of self-antigens to T cells, whereas it inhibited the activity of regulatory CD4 T cells. The understanding of the role of leptin in modulating autoimmune responses in SLE can open possibilities of leptin-targeted therapeutic intervention in the disease. PMID: 27588900
  21. in congenitally leptin deficient animals, leptin is not required for compensatory reduction in energy expenditure accompanying weight loss, but suggest that the hyperphagia of the weight-reduced state is leptin-dependent. PMID: 29261744
  22. Our data provide evidence that the interplay of these two hormones could help improve the understanding of the pathogenesis of atherosclerosis and NAFLD. PMID: 29158088
  23. Data suggest that Htr2c and leptin in hypothalamic nuclei may be involved in the effects of corticosterone on food intake/appetite regulation. (Htr2c = 5-hydroxytryptamine type 2C receptor) PMID: 28186389
  24. Further experiments indicated that miR-122 inhibited leptin-induced liver fibrosis in leptin-deficient mouse model. PMID: 29054053
  25. Endoplasmic reticulum stress-induced CHOP activation in the brain is a mechanistic link in the palmitate-induced negative regulation of leptin and IGF1. PMID: 27555288
  26. neuronal Rap1 critically regulates leptin sensitivity PMID: 27626668
  27. Upregulation of leptin levels in both the vessel wall and the circulation after vessel injury promoted the migration of Sca-1(+) progenitor cells via leptin receptor-dependent signal transducer and activator of transcription 3- Rac1/Cdc42-ERK (extracellular signal-regulated kinase)-FAK pathways, which enhanced neointimal formation. PMID: 28935755
  28. Depletion of CLPABP disturbed the normal subcellular localization of HuR to stress granules, and overexpression of CLPABP induced instability of leptin mRNA by inhibiting HuR function. PMID: 27616329
  29. These results confirm and extend the previous evidence that LEP has a general and important role in the response of mammalian cells to irradiation. PMID: 28593811
  30. leptin is involved in the proliferation and activation of microglia, which in turn enhances the development of neuropathic pain after avulsion of the cervical roots. PMID: 28822786
  31. Suggest that intact endothelial leptin signaling limits neointima formation and that obesity represents a state of endothelial leptin resistance. PMID: 28705795
  32. these studies demonstrate marked differences in the acute insulin-independent effects by which leptin reverses fasting hyperglycemia and ketoacidosis in a rodent model of DKA versus the chronic pleotropic effects by which leptin reverses hyperglycemia in a non-DKA rodent model of T1D. PMID: 28112679
  33. High fat diet attenuates leptin signaling throughout the brain, but some brain regions maintain their ability to sense leptin. Weight loss restores leptin sensing to some degree in most (but not all) brain regions, while other brain regions display hypersensitivity to leptin following weight loss PMID: 28107353
  34. CNS-specific Tak1 deletion prevented ER-stress-induced hypothalamic leptin resistance and hyperphagic obesity under a high-fat diet (HFD). Thus, TAK1 is a crucial regulator of ER stress in vivo, which could be a target for alleviation of ER stress and its associated disease conditions. PMID: 26985063
  35. these data unmasked a role for mitochondrial fission in leptin sensitivity and glucose sensing of POMC neurons. PMID: 28190775
  36. Obesity and leptin deficiency substantially decreased morphine metabolism and clearance. PMID: 27782940
  37. These findings suggest that by impairing the testicular LEP-JAK-STAT pathway, early-stage obesity inhibits the biosynthesis of testosterone and sexual development and reduces male reproductive potential. Long-term moderate and high-volume exercise can effectively reduce body fat and improve obesity-induced abnormalities in testicular leptin signal transduction, whereas only moderate-volume exercise can reverse the negativ PMID: 28100475
  38. genetic and pharmacological ablation of adult NG2-glia (also known as oligodendrocyte precursors), but not microglia, leads to primary leptin resistance and obesity in mice. PMID: 27166944
  39. a novel mechanism of leptin-induced fatty acid oxidation in muscle tissue; namely, this process is dependent on the activation of AMPK to induce the translocation of FAT/CD36 to the plasma membrane, thereby stimulating fatty acid uptake. PMID: 27827305
  40. Results demonstrate that in white adipose tissue, leptin expression and secretion is promoted by Epac1, hence regulating energy balance and glucose homeostasis. PMID: 27381457
  41. may regulate growth hormone expression in somatotropes through the stimulation of POU1F1 PMID: 27571135
  42. This study found that the expression of leptin and its receptor OB-R in mouse models of Sjogren's syndrome are elevated both locally and systemically during Sjogren's syndrome progression. PMID: 27889607
  43. obesity-associated elevation of leptin contributes to the increased susceptibility of asthma via modulation of pro-allergic lymphocyte responses. PMID: 27566543
  44. Leptin stimulation led to TNFalpha production under both in vitro and in vivo conditions, and diurnal fluctuation of leptin concentrations in the cerebrospinal fluid predicted the circadian changes of TNFalpha gene expression in the hypothalamus. PMID: 27226618
  45. geniposide may regulate tau phosphorylation through leptin signaling PMID: 26496899
  46. Activated FXR inhibits leptin signaling and counteracts tumor-promoting activities of cancer-associated fibroblasts in breast malignancy. PMID: 26899873
  47. this study shows that knockdown of leptin in hepatoma cells caused higher Treg activity and lower CD8+ T-cell response in the mouse hepatocellular carcinoma allograft model. PMID: 26639061
  48. Hexim1 selectively modulates leptin-mediated signal transduction pathways in the hypothalamus. PMID: 26859361
  49. The weight-reducing hypophagic effects of leptin are not augmented through its thermogenic effect. PMID: 26876182
  50. ime-specific changes in promoter associated DNA methyltransferases may be associated with the regulation of leptin expression. PMID: 25433105

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Involvement in disease Defects in Lep are the cause of profound obesity and type II diabetes.
Subcellular Location Secreted
Protein Families Leptin family
Database Links

KEGG: mmu:16846

STRING: 10090.ENSMUSP00000067046

UniGene: Mm.277072

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