Mouse Matrix Gla Protein(MGP)ELISA Kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

Matrix Gla Protein(MGP)

Alternative Names

Mgp ELISA Kit; Mglap ELISA Kit; Matrix Gla protein ELISA Kit; MGP ELISA Kit

Abbreviation

MGP

Uniprot No.

P19788

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	93-99
1:200	Average %	87
	Range %	85-89
1:400	Average %	91
	Range %	85-97
1:800	Average %	104
	Range %	102-106

Recovery

The recovery of mouse MGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	87	85-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.432	2.468	2.450	2.337
1000	2.169	2.150	2.160	2.047
500	1.704	1.735	1.720	1.607
250	1.115	1.136	1.126	1.013
125	0.568	0.598	0.583	0.470
62.5	0.344	0.368	0.356	0.243
31.25	0.226	0.236	0.231	0.118
0	0.114	0.112	0.113

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse MGP ELISA Kit was designed for the quantitative measurement of Mouse MGP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Background

Function
(From Uniprot)

Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.

Gene References into Functions

We propose MGP as a strong candidate for the regulation of stiffness in glaucoma. MGP further illustrates the presence of a common function affecting key glaucomatous parameters in the front and back of the eye, and thus offers the possibility for a sole therapeutic target for the disease. PMID: 27593913
the nasal septum to be abnormally mineralized and shortened in Mgp(-/-) mice. Transgenic restoration of Mgp expression in chondrocytes fully corrected the craniofacial anomalies caused by MGP deficiency, suggesting a local role for MGP in the developing nasal septum. PMID: 28487368
loss of MGP causes dysregulation of early endothelial differentiation. PMID: 26364300
Matrix Gla protein limits pulmonary arteriovenous malformations in ALK1 deficiency. PMID: 25614167
expression of MGP and OCN increased gradually in the murine developing tibial epiphysis, and the two mineral-associated proteins may occur at the same location during a particular period, but at different levels. PMID: 24956112
Matrix Gla's restricted expression to glaucoma-associated tissues from anterior and posterior segments suggests its involvement in the development of the disease. PMID: 25711639
Elastin haploinsufficiency impedes the progression of arterial calcification in MGP-deficient mice. PMID: 23857752
The arterial calcification, not MGP deficiency itself, causes the low bone mass phenotype in Mgp-/- mice. PMID: 23994172
Two sides of MGP null arterial disease: chondrogenic lesions dependent on transglutaminase 2 and elastin fragmentation associated with induction of adipsin. PMID: 24036114
MGP is a multi-functional inhibitor of normal and abnormal angiogenesis that may function by coordinating with both Notch and BMP signaling pathways PMID: 23110920
Mgp gene deletion may have a role in arteriovenous malformations PMID: 21765215
role in vascular calcification in vivo PMID: 12391016
MGP is a known inhibitor of mineralization, and mice deficient in Mgp show severe vascular calcification and premature bone mineralization. PMID: 16612082
Inorganic phosphate(Pi) regulates MGP expression in growth plate chondrocytes, thereby suggesting a key role for Pi and ERK1/2 in the regulation of bone formation. PMID: 17068135
PTH regulates MGP via both PKA- and ERK-dependent pathways. PMID: 17407158
Matrix GLA protein is an inhibitory morphogen in pulmonary vascular development PMID: 17670744
MGP in Abcc6-/- mice is largely in inactive form and is unable to prevent the unwanted mineralization of connective tissues in PXE. PMID: 17942075
Extensive hypomineralization was observed in dentin and alveolar bone from transgenic mice expressing matrix Gla protein when compared with that in wild-type tissues. Mineralization was virtually absent in tooth root dentin and cellular cementum. PMID: 18719210
Oral anticoagulant treatment decreased local expression of MGP. PMID: 19350115
Inorganic phosphate regulates MGP expression in osteoblasts through the ERK1/2-Fra-1 pathway. PMID: 19419315
Heat shock protein 70 enhances vascular bone morphogenetic protein-4 signaling by binding matrix Gla protein. PMID: 19661459

Hide All

Subcellular Location

Secreted.

Protein Families

Osteocalcin/matrix Gla protein family

Database Links

KEGG: mmu:17313

STRING: 10090.ENSMUSP00000032342

UniGene: Mm.243085

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-E16540m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.