Mouse Matrix Gla Protein(MGP)ELISA Kit

Instructions
Code CSB-E16540m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Matrix Gla Protein(MGP)
Alternative Names Mgp ELISA Kit; Mglap ELISA Kit; Matrix Gla protein ELISA Kit; MGP ELISA Kit
Abbreviation MGP
Uniprot No. P19788
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %96
Range %93-99
1:200Average %87
Range %85-89
1:400Average %91
Range %85-97
1:800Average %104
Range %102-106
Recovery
The recovery of mouse MGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8683-89
EDTA plasma (n=4)8785-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.432 2.468 2.450 2.337
10002.169 2.150 2.160 2.047
5001.704 1.735 1.720 1.607
2501.115 1.136 1.126 1.013
1250.568 0.598 0.583 0.470
62.50.344 0.368 0.356 0.243
31.250.226 0.236 0.231 0.118
00.114 0.112 0.113
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
Gene References into Functions
  1. We propose MGP as a strong candidate for the regulation of stiffness in glaucoma. MGP further illustrates the presence of a common function affecting key glaucomatous parameters in the front and back of the eye, and thus offers the possibility for a sole therapeutic target for the disease. PMID: 27593913
  2. the nasal septum to be abnormally mineralized and shortened in Mgp(-/-) mice. Transgenic restoration of Mgp expression in chondrocytes fully corrected the craniofacial anomalies caused by MGP deficiency, suggesting a local role for MGP in the developing nasal septum. PMID: 28487368
  3. loss of MGP causes dysregulation of early endothelial differentiation. PMID: 26364300
  4. Matrix Gla protein limits pulmonary arteriovenous malformations in ALK1 deficiency. PMID: 25614167
  5. expression of MGP and OCN increased gradually in the murine developing tibial epiphysis, and the two mineral-associated proteins may occur at the same location during a particular period, but at different levels. PMID: 24956112
  6. Matrix Gla's restricted expression to glaucoma-associated tissues from anterior and posterior segments suggests its involvement in the development of the disease. PMID: 25711639
  7. Elastin haploinsufficiency impedes the progression of arterial calcification in MGP-deficient mice. PMID: 23857752
  8. The arterial calcification, not MGP deficiency itself, causes the low bone mass phenotype in Mgp-/- mice. PMID: 23994172
  9. Two sides of MGP null arterial disease: chondrogenic lesions dependent on transglutaminase 2 and elastin fragmentation associated with induction of adipsin. PMID: 24036114
  10. MGP is a multi-functional inhibitor of normal and abnormal angiogenesis that may function by coordinating with both Notch and BMP signaling pathways PMID: 23110920
  11. Mgp gene deletion may have a role in arteriovenous malformations PMID: 21765215
  12. role in vascular calcification in vivo PMID: 12391016
  13. MGP is a known inhibitor of mineralization, and mice deficient in Mgp show severe vascular calcification and premature bone mineralization. PMID: 16612082
  14. Inorganic phosphate(Pi) regulates MGP expression in growth plate chondrocytes, thereby suggesting a key role for Pi and ERK1/2 in the regulation of bone formation. PMID: 17068135
  15. PTH regulates MGP via both PKA- and ERK-dependent pathways. PMID: 17407158
  16. Matrix GLA protein is an inhibitory morphogen in pulmonary vascular development PMID: 17670744
  17. MGP in Abcc6-/- mice is largely in inactive form and is unable to prevent the unwanted mineralization of connective tissues in PXE. PMID: 17942075
  18. Extensive hypomineralization was observed in dentin and alveolar bone from transgenic mice expressing matrix Gla protein when compared with that in wild-type tissues. Mineralization was virtually absent in tooth root dentin and cellular cementum. PMID: 18719210
  19. Oral anticoagulant treatment decreased local expression of MGP. PMID: 19350115
  20. Inorganic phosphate regulates MGP expression in osteoblasts through the ERK1/2-Fra-1 pathway. PMID: 19419315
  21. Heat shock protein 70 enhances vascular bone morphogenetic protein-4 signaling by binding matrix Gla protein. PMID: 19661459

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Subcellular Location Secreted
Protein Families Osteocalcin/matrix Gla protein family
Database Links

KEGG: mmu:17313

STRING: 10090.ENSMUSP00000032342

UniGene: Mm.243085

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