Mouse Neuron-specific enolase,NSE ELISA Kit

Instructions
Code CSB-E07962m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name enolase 2 (gamma, neuronal)
Alternative Names Eno2 ELISA Kit; Eno-2Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit
Abbreviation ENO2
Uniprot No. P17183
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %89-102
1:2Average %91
Range %87-95
1:4Average %94
Range %84-97
1:8Average %91
Range %85-94
Recovery
The recovery of mouse NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10592-108
EDTA plasma (n=4)9184-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.622 2.542 2.582 2.475
502.209 2.167 2.188 2.081
251.463 1.487 1.475 1.368
12.50.762 0.734 0.748 0.641
6.250.358 0.333 0.346 0.239
3.120.252 0.245 0.249 0.142
1.560.180 0.168 0.174 0.067
00.106 0.108 0.107
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Gene References into Functions
  1. Study analyzed the regions that regulate the promoter strength and cellular specificity of the 1.8 kb rNSE promoter in the cerebellum in vivo. The rNSE promoter was divided into three regions (A, B, and C). Our results showed that region C was indispensable for the promoter activity in mouse cerebellum and served as an interneuron-predominant basal promoter. PMID: 28508170
  2. Plaque-associated microglial cells express gamma-enolase and cathepsin X. PMID: 23621429
  3. Treadmill exercise training decreased the expression of NSN in the striatum of chronic Parkinsonian mice, which can partially explain the beneficial neuroprotective role of exercise in patients with parkinson disease. PMID: 21725169
  4. we induced differentiation of mouse stromal cells to neuron-like cells and observed the activation of tissue-specific promoter of neuron-specific enolase in transgenic mice with the human bcl-2 gene expressed only in neurons under the NSE promoter PMID: 14593212
Subcellular Location Cytoplasm, Cell membrane
Protein Families Enolase family
Tissue Specificity Skeletal muscle (at protein level). The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodi
Database Links

KEGG: mmu:13807

STRING: 10090.ENSMUSP00000004378

UniGene: Mm.3913

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